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The map label for this gene is rocR1 [H]

Identifier: 218232595

GI number: 218232595

Start: 479615

End: 481018

Strand: Reverse

Name: rocR1 [H]

Synonym: BCB4264_A0494

Alternate gene names: 218232595

Gene position: 481018-479615 (Counterclockwise)

Preceding gene: 218232325

Following gene: 218233338

Centisome position: 8.88

GC content: 33.33

Gene sequence:

>1404_bases
ATGCGTACAGAAGAAATTAATTTAAAACAGATCATTGAAATGAATATGCTATATGAAACTTTACTCAATGAACTCGACAT
CGGAATTCATATTATTAATGAGGAAAGTAAGACGATTATCTATAACCGCAAAATGATGGAAATTGAATCAATGGAGCGCT
CAGATGTGCTATATAAAAGTCCTTTAGAAGTATTCGCATTTGAAGAAAATAAAAATAGTACTCTTATAGAGGCATTAAAA
TTTGGAAAAACAAAGAAAAATATAAAACAAACGTATTTTAACAATAAAGGACAAGAAATTACAACGATTAACGATACTTT
CCCTATAATAGAAAATGGAAAAATTAAAGGCGCTATTGAAATATCAAAAGAAATTAGTAACTTAAAGCAAACGATAAAAA
TAGGCCCTTCTCGAAAACAAAGCACTAAATTTACCTTTGATCACATAATTGGTGATTCTGAAGCCATTCAATCAATCATT
ACAGAAGGAAAAAGAGTAATTCGTACATCTTCCTCCATACTTCTAGTAGGAGAAACAGGCACTGGTAAAGAACTATTTGC
ACAAAGCATCCATAATGAAAGTCAGCGTTCAACAAAACCCTTTATTTCACAAAACTGTGCCGCTATACCTGATACCTTAA
TGGAAAGCCTACTATTTGGCACAAATCGAGGTGCCTTTACAGGAGCAATAGATAAATCAGGCTTATTCGAAGAAGCAAAC
GGAGGAACTTTGTTATTAGATGAAATCAATTCATTAAGCCCAATACTTCAAGCTAAGTTGCTGCGGGCTATACAAGAAAA
AACAATACGAAGAATTGGAGGTACACACGAAAAAGAAATTGATGTTCGCATTATAGCAACTATCAATGAAGATCCCTTTG
AAGCCATCGCACATAATCGATTAAGAGAAGATTTATATTACCGATTAAGCGTCGTTACTTTATGTCTTCCGCCTTTACGT
GAAAGAAAAGAAGATATTCCAGCTCTTGTTCAGCACTTTATTGAAAAATATAATACTCAATTCGGGCTCAACGTAACCGA
TGTAGATGTAAATGTAAGAGAATTTTTCTATGCATACGATTGGCCTGGAAACGTACGGGAGTTGGAACATATAATTGAAG
GTTCCATGAACTTGATAGAAGATGAAACCATCATTACAGCGTTTCATCTTCCTACTCGCTTTCGCGAACGATTAAAAAAA
GAATTCAATATGCAACCTCTCCTAATTAATAACGATACTGATGCGCCAAAAACGTTAAAGCACAAAATAGCAGAAATGGA
AAAGAACTACATCAATCAAATTTTGAAAGAGTATCACGGTAATATCTCACAAGCTGCAAAATTTTTAGGATTAAGTAGGC
AAAACTTACAATATCGAATTAAAAAACTGCATTTACACATATGA

Upstream 100 bases:

>100_bases
TTAAAAGGAAAATAGCAAAAAATTTTTGCGATTTTATAAGAATTTTAATAAAATTATAAAAAGTCAGTCTTCATAGGGTG
GAATGAGGAGAAGATAATCT

Downstream 100 bases:

>100_bases
AATAAACATGTCTCCAAGTGCAAAAACAACTTGGTAAAATATGTTATAATAAACATATAACATGCTAGAAAGGGGCTATA
TATATGAGTAATGATAAAAA

Product: arginine utilization regulatory protein RocR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
FGKTKKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEISNLKQTIKIGPSRKQSTKFTFDHIIGDSEAIQSII
TEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKSGLFEEAN
GGTLLLDEINSLSPILQAKLLRAIQEKTIRRIGGTHEKEIDVRIIATINEDPFEAIAHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNTQFGLNVTDVDVNVREFFYAYDWPGNVRELEHIIEGSMNLIEDETIITAFHLPTRFRERLKK
EFNMQPLLINNDTDAPKTLKHKIAEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Sequences:

>Translated_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
FGKTKKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEISNLKQTIKIGPSRKQSTKFTFDHIIGDSEAIQSII
TEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKSGLFEEAN
GGTLLLDEINSLSPILQAKLLRAIQEKTIRRIGGTHEKEIDVRIIATINEDPFEAIAHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNTQFGLNVTDVDVNVREFFYAYDWPGNVRELEHIIEGSMNLIEDETIITAFHLPTRFRERLKK
EFNMQPLLINNDTDAPKTLKHKIAEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
>Mature_467_residues
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKSPLEVFAFEENKNSTLIEALK
FGKTKKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIEISKEISNLKQTIKIGPSRKQSTKFTFDHIIGDSEAIQSII
TEGKRVIRTSSSILLVGETGTGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKSGLFEEAN
GGTLLLDEINSLSPILQAKLLRAIQEKTIRRIGGTHEKEIDVRIIATINEDPFEAIAHNRLREDLYYRLSVVTLCLPPLR
ERKEDIPALVQHFIEKYNTQFGLNVTDVDVNVREFFYAYDWPGNVRELEHIIEGSMNLIEDETIITAFHLPTRFRERLKK
EFNMQPLLINNDTDAPKTLKHKIAEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=345, Percent_Identity=40, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1790437, Length=319, Percent_Identity=39.1849529780564, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=39.938080495356, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1789233, Length=338, Percent_Identity=38.4615384615385, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1788905, Length=317, Percent_Identity=37.5394321766561, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=39.7515527950311, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=38.6706948640483, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1790299, Length=337, Percent_Identity=36.7952522255193, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1787583, Length=348, Percent_Identity=32.183908045977, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=34.4311377245509, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=32.1100917431193, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081858, Length=445, Percent_Identity=27.8651685393258, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789828, Length=215, Percent_Identity=37.2093023255814, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53629; Mature: 53629

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKFGKTKKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEECCCEEEHHH
ISKEISNLKQTIKIGPSRKQSTKFTFDHIIGDSEAIQSIITEGKRVIRTSSSILLVGETG
HHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHCCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKSGLFEEAN
CCHHHHHHHHHCHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPILQAKLLRAIQEKTIRRIGGTHEKEIDVRIIATINEDPFEAIAHNR
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNTQFGLNVTDVDVNVREFFYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHEEEEEC
WPGNVRELEHIIEGSMNLIEDETIITAFHLPTRFRERLKKEFNMQPLLINNDTDAPKTLK
CCCCHHHHHHHHHCCHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEECCCCCHHHHH
HKIAEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTEEINLKQIIEMNMLYETLLNELDIGIHIINEESKTIIYNRKMMEIESMERSDVLYKS
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCCCHHCC
PLEVFAFEENKNSTLIEALKFGKTKKNIKQTYFNNKGQEITTINDTFPIIENGKIKGAIE
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCEECCCEEEHHH
ISKEISNLKQTIKIGPSRKQSTKFTFDHIIGDSEAIQSIITEGKRVIRTSSSILLVGETG
HHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHCCHHEEEECCCEEEEECCC
TGKELFAQSIHNESQRSTKPFISQNCAAIPDTLMESLLFGTNRGAFTGAIDKSGLFEEAN
CCHHHHHHHHHCHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
GGTLLLDEINSLSPILQAKLLRAIQEKTIRRIGGTHEKEIDVRIIATINEDPFEAIAHNR
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHH
LREDLYYRLSVVTLCLPPLRERKEDIPALVQHFIEKYNTQFGLNVTDVDVNVREFFYAYD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHEEEEEC
WPGNVRELEHIIEGSMNLIEDETIITAFHLPTRFRERLKKEFNMQPLLINNDTDAPKTLK
CCCCHHHHHHHHHCCHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCEEEECCCCCHHHHH
HKIAEMEKNYINQILKEYHGNISQAAKFLGLSRQNLQYRIKKLHLHI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]