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The map label for this gene is proP [H]

Identifier: 218232145

GI number: 218232145

Start: 2890212

End: 2891669

Strand: Reverse

Name: proP [H]

Synonym: BCB4264_A3018

Alternate gene names: 218232145

Gene position: 2891669-2890212 (Counterclockwise)

Preceding gene: 218233821

Following gene: 218235188

Centisome position: 53.36

GC content: 36.42

Gene sequence:

>1458_bases
ATGCAACAAAATAATGATTTAAATTTTGAACCTCAAGACGTTAATATTGTTAATCCTAAACAAGCCAGGAAAGCAGTAAT
TGCTACTGGTATAGGGAACGCAATGGAGTGGTTTGACTTCGGATTATACGCGTATTTAGCGGTAATTTTAAGTCAATTAT
TCTTCTCTGGTGTTGATAATAGTGGATTGCAACTTGTACTTACATTCGGTACATTTGCAGCAGCCTTTCTCGTTCGACCA
ATCGGAGGTGTATTCTTTGGTAGAATAGGAGATAAGTACGGCCGAAAAATCGTGTTAAGTACTACTATTATTTTAATGGC
ACTTTCTACATTATTCATCGCATTACTACCAACCTATGAACAAATTGGTGTATGGGCACCAATACTACTTTTAGTTGCCC
GAATGATTCAAGGCTTCTCTACAGGCGGCGAATATTCAGGAGCAATGGTTTATATCGCAGAATCTTCTCCAGATAAAAAG
CGTGGTATACTCGGTAGTGGTCTTGAAATTGGAACACTCTCAGGTTACATTGCTGCATCGGTAATTGTTACCATTTTGAC
GTTATTGTTAACAGATGAGCAAATGCTCAGCTGGGGATGGCGTATTCCGTTCTTAATAGCTGCACCAATTGGTTTGGTCG
GTTTATACTTACGCCGTCATCTAGATGAATCTCCAATCTTTGAAGAGATGGAAAAAGCACAAGAGGAATCTGAAGACAAT
GAACAATTTTCATTCATGGATATTATTAAATATCACAAAAAAGACTTCTTATTAAGCACAGTAATTGTCGCCTTCTTTAA
TATTACAAATTATATGATTCTTTCGTATATTCCTTCTTATCTAACTCAAGTACTTAAAGTCGAAGAAACAACAGGCTTAT
TAATTATTTCTATTACGATGGCACTTATGATTCCACTAGCACTATATTTCGGTAAATTAAGTGATAAAATTGGTAATAAA
CGCGTTGTGCAAATTGGTTTACTTGGTTTAACTGTATTTGCAATTCCAGCATTTTTACTAATAGGTAACGGACATATTGC
AGCTATATTTGCAGGTATTTTCGTACTAGGTTTCTTCTTAAGTGTATATGAAGGAACATTACCTTCATTATTACCATCAC
TCTTTTTCACTGATGTACGTTATCGAGCACTTTCGATCTCATTTAATATTTCTGTATCGATATTCGGTGGAACAACACCG
CTCGTATGTTCATACTTAGTTCATGCAACTGGTAACCCGCTCGCACCGGCATTTTATTTAGCAGGTGTAAGTATTATTGG
ATTAGTTGTATTTAGTGTACTATTCGTTACAACTTCAGGGCGTGCGCTAAAAGGTTCATATCCTACAGTAGAAACGAAAA
AAGAAGCACACCAAATCGCTAAAGAAGACCCTGAAGAAACACTTTGGTGGCATGAAGAGTCATTAGAAATTGAAGCAGGG
AAGAATGCTTCCATTTAA

Upstream 100 bases:

>100_bases
AAGGAGACGTTGCTTTTCCTTAAGTGCTTCGAATTAATTTTATTCAATAAGAGAAGGTGTGCTTAAAGAAGTTAAATTAA
ATAAGGAGCTGTTTTATTAT

Downstream 100 bases:

>100_bases
AAAGCAACGTAGAAGTACAAAAAGACTCCTATGAATATAGGAGTCTTTTTGTTACGTTATTTCTTTCGTTTCTTCAAACT
TTTTTGATATGCATAAAGCT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQQNNDLNFEPQDVNIVNPKQARKAVIATGIGNAMEWFDFGLYAYLAVILSQLFFSGVDNSGLQLVLTFGTFAAAFLVRP
IGGVFFGRIGDKYGRKIVLSTTIILMALSTLFIALLPTYEQIGVWAPILLLVARMIQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASVIVTILTLLLTDEQMLSWGWRIPFLIAAPIGLVGLYLRRHLDESPIFEEMEKAQEESEDN
EQFSFMDIIKYHKKDFLLSTVIVAFFNITNYMILSYIPSYLTQVLKVEETTGLLIISITMALMIPLALYFGKLSDKIGNK
RVVQIGLLGLTVFAIPAFLLIGNGHIAAIFAGIFVLGFFLSVYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGTTP
LVCSYLVHATGNPLAPAFYLAGVSIIGLVVFSVLFVTTSGRALKGSYPTVETKKEAHQIAKEDPEETLWWHEESLEIEAG
KNASI

Sequences:

>Translated_485_residues
MQQNNDLNFEPQDVNIVNPKQARKAVIATGIGNAMEWFDFGLYAYLAVILSQLFFSGVDNSGLQLVLTFGTFAAAFLVRP
IGGVFFGRIGDKYGRKIVLSTTIILMALSTLFIALLPTYEQIGVWAPILLLVARMIQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASVIVTILTLLLTDEQMLSWGWRIPFLIAAPIGLVGLYLRRHLDESPIFEEMEKAQEESEDN
EQFSFMDIIKYHKKDFLLSTVIVAFFNITNYMILSYIPSYLTQVLKVEETTGLLIISITMALMIPLALYFGKLSDKIGNK
RVVQIGLLGLTVFAIPAFLLIGNGHIAAIFAGIFVLGFFLSVYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGTTP
LVCSYLVHATGNPLAPAFYLAGVSIIGLVVFSVLFVTTSGRALKGSYPTVETKKEAHQIAKEDPEETLWWHEESLEIEAG
KNASI
>Mature_485_residues
MQQNNDLNFEPQDVNIVNPKQARKAVIATGIGNAMEWFDFGLYAYLAVILSQLFFSGVDNSGLQLVLTFGTFAAAFLVRP
IGGVFFGRIGDKYGRKIVLSTTIILMALSTLFIALLPTYEQIGVWAPILLLVARMIQGFSTGGEYSGAMVYIAESSPDKK
RGILGSGLEIGTLSGYIAASVIVTILTLLLTDEQMLSWGWRIPFLIAAPIGLVGLYLRRHLDESPIFEEMEKAQEESEDN
EQFSFMDIIKYHKKDFLLSTVIVAFFNITNYMILSYIPSYLTQVLKVEETTGLLIISITMALMIPLALYFGKLSDKIGNK
RVVQIGLLGLTVFAIPAFLLIGNGHIAAIFAGIFVLGFFLSVYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGTTP
LVCSYLVHATGNPLAPAFYLAGVSIIGLVVFSVLFVTTSGRALKGSYPTVETKKEAHQIAKEDPEETLWWHEESLEIEAG
KNASI

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=456, Percent_Identity=43.2017543859649, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=30.8924485125858, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789941, Length=415, Percent_Identity=33.9759036144578, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788942, Length=398, Percent_Identity=30.4020100502513, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87082404, Length=453, Percent_Identity=26.9315673289183, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082231, Length=445, Percent_Identity=23.3707865168539, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53221; Mature: 53221

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNNDLNFEPQDVNIVNPKQARKAVIATGIGNAMEWFDFGLYAYLAVILSQLFFSGVDN
CCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGLQLVLTFGTFAAAFLVRPIGGVFFGRIGDKYGRKIVLSTTIILMALSTLFIALLPTYE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCHH
QIGVWAPILLLVARMIQGFSTGGEYSGAMVYIAESSPDKKRGILGSGLEIGTLSGYIAAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHH
VIVTILTLLLTDEQMLSWGWRIPFLIAAPIGLVGLYLRRHLDESPIFEEMEKAQEESEDN
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
EQFSFMDIIKYHKKDFLLSTVIVAFFNITNYMILSYIPSYLTQVLKVEETTGLLIISITM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALMIPLALYFGKLSDKIGNKRVVQIGLLGLTVFAIPAFLLIGNGHIAAIFAGIFVLGFFL
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SVYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGTTPLVCSYLVHATGNPLAPAFYL
HHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHH
AGVSIIGLVVFSVLFVTTSGRALKGSYPTVETKKEAHQIAKEDPEETLWWHEESLEIEAG
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECC
KNASI
CCCCC
>Mature Secondary Structure
MQQNNDLNFEPQDVNIVNPKQARKAVIATGIGNAMEWFDFGLYAYLAVILSQLFFSGVDN
CCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SGLQLVLTFGTFAAAFLVRPIGGVFFGRIGDKYGRKIVLSTTIILMALSTLFIALLPTYE
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHCCHH
QIGVWAPILLLVARMIQGFSTGGEYSGAMVYIAESSPDKKRGILGSGLEIGTLSGYIAAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCCHHHHHHHHH
VIVTILTLLLTDEQMLSWGWRIPFLIAAPIGLVGLYLRRHLDESPIFEEMEKAQEESEDN
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
EQFSFMDIIKYHKKDFLLSTVIVAFFNITNYMILSYIPSYLTQVLKVEETTGLLIISITM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ALMIPLALYFGKLSDKIGNKRVVQIGLLGLTVFAIPAFLLIGNGHIAAIFAGIFVLGFFL
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SVYEGTLPSLLPSLFFTDVRYRALSISFNISVSIFGGTTPLVCSYLVHATGNPLAPAFYL
HHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCHHHHH
AGVSIIGLVVFSVLFVTTSGRALKGSYPTVETKKEAHQIAKEDPEETLWWHEESLEIEAG
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEEECC
KNASI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]