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The map label for this gene is potH [H]

Identifier: 209552163

GI number: 209552163

Start: 270606

End: 271850

Strand: Reverse

Name: potH [H]

Synonym: Rleg2_6320

Alternate gene names: 209552163

Gene position: 271850-270606 (Counterclockwise)

Preceding gene: 209552164

Following gene: 209552162

Centisome position: 88.05

GC content: 58.31

Gene sequence:

>1245_bases
ATGGTCGCCATCACGGAACTTACAACGGCAGATGGCCGCCCGCTTCGGACTGCTTTGGCAGACGCTCGAATCAGGGCGAA
GCGAAGAGCGCTGTTACTCGTCTTGCCGCTTTTACTGTTCATCGTAATCACATTCGTCGTGCCGATCGGGCAGGTCCTGT
ACAGATCGTTTTACAATGACGAATTTGCCGCGAGCGCCCCGCAGCTAAAGGCATGGTTCGAGGCAACAGCGGAAGGCACA
GCCCCAGACGAGGCTGCTTACGGCGCACTCGCCGCGGATCTTGACCGGATGCGACGCGAGCGGACCGCTGGTGCCGCAGG
CACGAGAATAAACGATGTCCTTCCCGGCTCGAGATCGCTGTTCACTGCGGCTGCCAGAGGCGCTGTCGAGTTGAAGCCAC
CATATCGAGAAGCGCTCGTTGAAAAAGACGCTCGCTGGGAAGATCCGCGTCTCTGGAACGCCATGCGCGCAGCTGTAAAT
CCGAAAACGGCGGACTACTTCATCATCGCCCTGGATCGACAGCGGAGCGTTGACGGCGGCTGGGAGCGGGCTCCGGAGGA
GCGCCGGCTGTATGTATCTCTGTTCATTAAGACCCTCTGGCAGTCCGGCCTGGTTACTTTACTCTGCCTAGTCCTGGCAT
TCCCGGTCGCTCACCTCATCGCGAACCTGCGCCCGTCGATCGCGAACCTGCTTCTTATGCTCGTGTTGCTACCGTTCTGG
ACCTCGTTGCTCGTCCGAACATCAAGCTGGATCGTACTGCTTCAGGGGCAGGGTGTCGTGAACAACATCCTCGTCGCTAT
CGACCTGGTCAAAACTGATCGAAGGCTCGAGTTGATCTACAACAACGTCGGAACCATCATCGTCATGGTGCACGTGTCGC
TTCCCTTGATGATTCTTCCCCTGTATTCGGTGATGCGGATGATAAATCCTGCCTATGTGCGGGCGGCCCGCAGCCTCGGA
GCCACGAGCTGGACGGCTTTCAGGCGCGTCTACCTGCCTTTGACGATGCCGGGTGTCGCCGCTGGGTGTCTGCTCGTCTA
CATTCTCGCAGTCGGTTATTACATCACACCAGCGCTCGTCGGCGGAGCGGACGGGCAGTTCATTTCGAACATGATCCAAT
ACCATCTTGGGGCATCGAACTGGTCTCTTGCCGCCGCCTTGGCGCTTATCCTCCTCGTCGGTGTCTTGATCCTATACTGG
CTCTATGACCGCCTGGTTGGGATCGAGAAACTCAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
GAATTGACGGAGCGCTTCAACGCGTGGCTCGCCACGAATTGATGTATCGCCCGGCGGACCGCGCTCGGTCCGCCGGATCA
CATTCAAGACTGGAAGACTG

Downstream 100 bases:

>100_bases
TCGCCTGCGGCCAAGACCGTCCTACCGGAAAACGAACATGAAAACTTCGCAGCACGCCGACACATTAGAGAAGACATGGA
GCTGGATCTATCAGGCGATC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVAITELTTADGRPLRTALADARIRAKRRALLLVLPLLLFIVITFVVPIGQVLYRSFYNDEFAASAPQLKAWFEATAEGT
APDEAAYGALAADLDRMRRERTAGAAGTRINDVLPGSRSLFTAAARGAVELKPPYREALVEKDARWEDPRLWNAMRAAVN
PKTADYFIIALDRQRSVDGGWERAPEERRLYVSLFIKTLWQSGLVTLLCLVLAFPVAHLIANLRPSIANLLLMLVLLPFW
TSLLVRTSSWIVLLQGQGVVNNILVAIDLVKTDRRLELIYNNVGTIIVMVHVSLPLMILPLYSVMRMINPAYVRAARSLG
ATSWTAFRRVYLPLTMPGVAAGCLLVYILAVGYYITPALVGGADGQFISNMIQYHLGASNWSLAAALALILLVGVLILYW
LYDRLVGIEKLKLG

Sequences:

>Translated_414_residues
MVAITELTTADGRPLRTALADARIRAKRRALLLVLPLLLFIVITFVVPIGQVLYRSFYNDEFAASAPQLKAWFEATAEGT
APDEAAYGALAADLDRMRRERTAGAAGTRINDVLPGSRSLFTAAARGAVELKPPYREALVEKDARWEDPRLWNAMRAAVN
PKTADYFIIALDRQRSVDGGWERAPEERRLYVSLFIKTLWQSGLVTLLCLVLAFPVAHLIANLRPSIANLLLMLVLLPFW
TSLLVRTSSWIVLLQGQGVVNNILVAIDLVKTDRRLELIYNNVGTIIVMVHVSLPLMILPLYSVMRMINPAYVRAARSLG
ATSWTAFRRVYLPLTMPGVAAGCLLVYILAVGYYITPALVGGADGQFISNMIQYHLGASNWSLAAALALILLVGVLILYW
LYDRLVGIEKLKLG
>Mature_414_residues
MVAITELTTADGRPLRTALADARIRAKRRALLLVLPLLLFIVITFVVPIGQVLYRSFYNDEFAASAPQLKAWFEATAEGT
APDEAAYGALAADLDRMRRERTAGAAGTRINDVLPGSRSLFTAAARGAVELKPPYREALVEKDARWEDPRLWNAMRAAVN
PKTADYFIIALDRQRSVDGGWERAPEERRLYVSLFIKTLWQSGLVTLLCLVLAFPVAHLIANLRPSIANLLLMLVLLPFW
TSLLVRTSSWIVLLQGQGVVNNILVAIDLVKTDRRLELIYNNVGTIIVMVHVSLPLMILPLYSVMRMINPAYVRAARSLG
ATSWTAFRRVYLPLTMPGVAAGCLLVYILAVGYYITPALVGGADGQFISNMIQYHLGASNWSLAAALALILLVGVLILYW
LYDRLVGIEKLKLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=34.0909090909091, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787369, Length=222, Percent_Identity=35.5855855855856, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787713, Length=209, Percent_Identity=32.0574162679426, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45772; Mature: 45772

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAITELTTADGRPLRTALADARIRAKRRALLLVLPLLLFIVITFVVPIGQVLYRSFYND
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EFAASAPQLKAWFEATAEGTAPDEAAYGALAADLDRMRRERTAGAAGTRINDVLPGSRSL
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
FTAAARGAVELKPPYREALVEKDARWEDPRLWNAMRAAVNPKTADYFIIALDRQRSVDGG
HHHHHCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
WERAPEERRLYVSLFIKTLWQSGLVTLLCLVLAFPVAHLIANLRPSIANLLLMLVLLPFW
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TSLLVRTSSWIVLLQGQGVVNNILVAIDLVKTDRRLELIYNNVGTIIVMVHVSLPLMILP
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHH
LYSVMRMINPAYVRAARSLGATSWTAFRRVYLPLTMPGVAAGCLLVYILAVGYYITPALV
HHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGADGQFISNMIQYHLGASNWSLAAALALILLVGVLILYWLYDRLVGIEKLKLG
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MVAITELTTADGRPLRTALADARIRAKRRALLLVLPLLLFIVITFVVPIGQVLYRSFYND
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EFAASAPQLKAWFEATAEGTAPDEAAYGALAADLDRMRRERTAGAAGTRINDVLPGSRSL
HHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHH
FTAAARGAVELKPPYREALVEKDARWEDPRLWNAMRAAVNPKTADYFIIALDRQRSVDGG
HHHHHCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
WERAPEERRLYVSLFIKTLWQSGLVTLLCLVLAFPVAHLIANLRPSIANLLLMLVLLPFW
HHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
TSLLVRTSSWIVLLQGQGVVNNILVAIDLVKTDRRLELIYNNVGTIIVMVHVSLPLMILP
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHH
LYSVMRMINPAYVRAARSLGATSWTAFRRVYLPLTMPGVAAGCLLVYILAVGYYITPALV
HHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GGADGQFISNMIQYHLGASNWSLAAALALILLVGVLILYWLYDRLVGIEKLKLG
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]