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The map label for this gene is potC [H]

Identifier: 209552162

GI number: 209552162

Start: 269633

End: 270568

Strand: Reverse

Name: potC [H]

Synonym: Rleg2_6319

Alternate gene names: 209552162

Gene position: 270568-269633 (Counterclockwise)

Preceding gene: 209552163

Following gene: 209552161

Centisome position: 87.63

GC content: 56.3

Gene sequence:

>936_bases
ATGAAAACTTCGCAGCACGCCGACACATTAGAGAAGACATGGAGCTGGATCTATCAGGCGATCTGCATCGCTGTCTTCTT
CTTCCTTGTTGCCCCGGTATTGATCGTAATTCCGCTTAGCTTCAACGCAGAACCGTATTTTTCCTTCACCCAGAAAATGC
TCGCTCTCGACCCCAAGGGCTTCTCGCTTCGTTGGTACGAGCTTCTCGCTACATTTGGAATGGCAAACCCTGGAGCCCCA
TTCGGCGGGGCTTGGTTAAGCGATCTTTGGAATAACTCGACGTGGCTGAACGCCGCCAAGAACTCATTCTTCGTGGGGAT
ATGTGCAACGATCATGTCCACCGTTCTCGGTACCTTGGCCGCCCTCGGCATGACGCGACAGGAAATGCCTTTCAAGCGTA
CGATCACGGCCATCCTGCTCGCTCCGATGATCGTACCGGTCGTCATCGTCGCGACGGGGCTTTTTTTCTTCTACTCCAAT
CCGTGTGGAATCCTCGGCTTCGAGTGTGGACGACTGACATCAACGTATGCGGGACTCATACTGGCGCATACAGCGCTTGG
CGTTCCCTTCGTACTCATAACCGTCAAGGCGACACTGACGGGGTTTGACGACACTCTTGCCCGAGCGGCGGCGAGTCTGG
GAGCGTCGCCGTCTCGGACCTTCTTCAAGGTCGTCATGCCACTGATCCTACCTGGCGTCGTATCCGGGGCTCTGTTTGCG
TTCGCCACTTCATTTGACGAAGTCGTAACGGTGCTATTCGTCGGCGGCCCTCAGCAAGTTACCATTCCCCGGCAGATGTG
GGGAGGTCTCCGCGAACAGGTTAGCCCCGCTATTCTCGCCGCCGCCACCATTCTTGTACTGATATCGGTGGCGTTGCTCA
CGACTGTGGAGCTTCTGCGCAGGCGCTCAGAACGCCTCCGGGGAATTCGCGCTTAG

Upstream 100 bases:

>100_bases
GTCTTGATCCTATACTGGCTCTATGACCGCCTGGTTGGGATCGAGAAACTCAAGCTCGGCTGATCGCCTGCGGCCAAGAC
CGTCCTACCGGAAAACGAAC

Downstream 100 bases:

>100_bases
CCACTGGACGAGGCGACCGCCGGGGGGATTGCCTCTCGTTCCGTGAGCAATCTAGCTATACACGGCGATCGAGGGCACGA
GCCAGTGGACCACTAGACGA

Product: binding-protein-dependent transporters inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKTSQHADTLEKTWSWIYQAICIAVFFFLVAPVLIVIPLSFNAEPYFSFTQKMLALDPKGFSLRWYELLATFGMANPGAP
FGGAWLSDLWNNSTWLNAAKNSFFVGICATIMSTVLGTLAALGMTRQEMPFKRTITAILLAPMIVPVVIVATGLFFFYSN
PCGILGFECGRLTSTYAGLILAHTALGVPFVLITVKATLTGFDDTLARAAASLGASPSRTFFKVVMPLILPGVVSGALFA
FATSFDEVVTVLFVGGPQQVTIPRQMWGGLREQVSPAILAAATILVLISVALLTTVELLRRRSERLRGIRA

Sequences:

>Translated_311_residues
MKTSQHADTLEKTWSWIYQAICIAVFFFLVAPVLIVIPLSFNAEPYFSFTQKMLALDPKGFSLRWYELLATFGMANPGAP
FGGAWLSDLWNNSTWLNAAKNSFFVGICATIMSTVLGTLAALGMTRQEMPFKRTITAILLAPMIVPVVIVATGLFFFYSN
PCGILGFECGRLTSTYAGLILAHTALGVPFVLITVKATLTGFDDTLARAAASLGASPSRTFFKVVMPLILPGVVSGALFA
FATSFDEVVTVLFVGGPQQVTIPRQMWGGLREQVSPAILAAATILVLISVALLTTVELLRRRSERLRGIRA
>Mature_311_residues
MKTSQHADTLEKTWSWIYQAICIAVFFFLVAPVLIVIPLSFNAEPYFSFTQKMLALDPKGFSLRWYELLATFGMANPGAP
FGGAWLSDLWNNSTWLNAAKNSFFVGICATIMSTVLGTLAALGMTRQEMPFKRTITAILLAPMIVPVVIVATGLFFFYSN
PCGILGFECGRLTSTYAGLILAHTALGVPFVLITVKATLTGFDDTLARAAASLGASPSRTFFKVVMPLILPGVVSGALFA
FATSFDEVVTVLFVGGPQQVTIPRQMWGGLREQVSPAILAAATILVLISVALLTTVELLRRRSERLRGIRA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=258, Percent_Identity=27.906976744186, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787081, Length=293, Percent_Identity=28.3276450511945, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787080, Length=115, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787714, Length=273, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788764, Length=224, Percent_Identity=28.125, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33699; Mature: 33699

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSQHADTLEKTWSWIYQAICIAVFFFLVAPVLIVIPLSFNAEPYFSFTQKMLALDPKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
FSLRWYELLATFGMANPGAPFGGAWLSDLWNNSTWLNAAKNSFFVGICATIMSTVLGTLA
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
ALGMTRQEMPFKRTITAILLAPMIVPVVIVATGLFFFYSNPCGILGFECGRLTSTYAGLI
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
LAHTALGVPFVLITVKATLTGFDDTLARAAASLGASPSRTFFKVVMPLILPGVVSGALFA
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FATSFDEVVTVLFVGGPQQVTIPRQMWGGLREQVSPAILAAATILVLISVALLTTVELLR
HHHHHHHHHHHHEECCCCCEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRSERLRGIRA
HHHHHHCCCCC
>Mature Secondary Structure
MKTSQHADTLEKTWSWIYQAICIAVFFFLVAPVLIVIPLSFNAEPYFSFTQKMLALDPKG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
FSLRWYELLATFGMANPGAPFGGAWLSDLWNNSTWLNAAKNSFFVGICATIMSTVLGTLA
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
ALGMTRQEMPFKRTITAILLAPMIVPVVIVATGLFFFYSNPCGILGFECGRLTSTYAGLI
HHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHH
LAHTALGVPFVLITVKATLTGFDDTLARAAASLGASPSRTFFKVVMPLILPGVVSGALFA
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FATSFDEVVTVLFVGGPQQVTIPRQMWGGLREQVSPAILAAATILVLISVALLTTVELLR
HHHHHHHHHHHHEECCCCCEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRSERLRGIRA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]