Click here to switch to the map view.

The map label for this gene is mdtE [H]

Identifier: 209550731

GI number: 209550731

Start: 3234991

End: 3236202

Strand: Direct

Name: mdtE [H]

Synonym: Rleg2_3155

Alternate gene names: 209550731

Gene position: 3234991-3236202 (Clockwise)

Preceding gene: 209550730

Following gene: 209550732

Centisome position: 71.29

GC content: 63.28

Gene sequence:

>1212_bases
ATGATACGGCGTGCCCTTCTCATCTCCTCGGCGCTGGCGTTCGGTGCACTGCTCGCAGCCTGCAGCGACAACGCCAGCAA
GCCCGCCGGCAACGCAGGCGCTGCCGCGGCCCAGCAGGCCTCGCCGGTTGGCGTGATCACGCTGAATAAGGGTAGTTTTC
CGGTCACCACGATCCTGCCGGGCCGCGCCGAGACCTTCCAGACGGCCGATATCCGGCCGCAGGTGAGCGGCCTGATCCGC
GAGATCGCCTTCAAGGAAGGCGGCGAGATCAAGAAGGGCGACCTTCTCTACCAGATCGAGGATGCGCCCTATATCGCCGC
CGTCGAGCAGGCCAAAGCGGCAATCTCCAAGGCCGAGGCGAGTGTGCCGAGCGCTGAAAGCAATCTCGACCGCTACCAGC
GCTTGGTCGGCAGCGGCGCCACCCAGATCGAATTCGAAACCGCCAAGACGACGCTGCTCCAGGCCAAGGCCGAAGTCGAG
TCCGCAAAGGCCGCGCTGTCCGCTGCGCAGATCGACCTCGACCACACCAGGATCATCGCCCCCTTCGACGGCATCATCGA
CCAGACCGCCTATAATGTCGGCAACGTCGTCTCCGCCAATCAGACGACGGCGCTGACGACGATCCGCCAGCTTGATCCGA
TCTACATTTCGCTGACGGAATCGAGCACCAATCTCTTGAAGCTGCGCGATGCGATGGCCGCCGGCGACATCAAGGGTGCA
GCCAATGTCGTCTTCCACCTGATCCTCGAAGACGGCAAGGAATACAACCAGCAGGGCAAGCTCGACATGTCGAAGCAGGT
GGTCAGTGAGACCACGGGCACCTTCATCATCCGCGTGCTCTTTCCCAATCCCGACCGGGTCGTCCTGCCCGGCATGTATG
TCCGGGCGACTGTCGAACTCGGCGCCGAGAACGGTTATGCGCTGCCGCAGCTGGCGACGAGCCGTGACGCCAACGGCCGG
CTGACGGCGCAATTCGTTTCGGCCGAAGGCAAGGTCGAAACCCGTGCTTTCGAAAACAGCTCGCCCTCCAACAATTCCTG
GCTCGTGACCGAAGGCATCAAGGACGGCGATCAGCTGATCGTCAGCGGCCTGCAATCGATCACATCAGGCATGCCGGTGA
AGCCGGTCCCGATGAAGATCACCGACAACGGCGTGGTCGTGGCTGCCGAGCAGCCCGCGGCCGGTGACGCACAGAAGCCC
GCAGCGAAGTAA

Upstream 100 bases:

>100_bases
CATTTGCAGCCAGATCAATCCTGACGCTTCTATAAGCCTTCGCTCGGCTGGGCCGAGCCCGTGGATCGAACGGCCATTGC
CACCGCACAGGGAATTCAGA

Downstream 100 bases:

>100_bases
TCGCTTCGAGCCCCGACGCCACCGTCTCAGCCGCACAGGAACAACCGATGGCCAAGTTTTTCATCCGACGTCCGATTTTC
GCCTGGGTCATTGCGATCAC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MIRRALLISSALAFGALLAACSDNASKPAGNAGAAAAQQASPVGVITLNKGSFPVTTILPGRAETFQTADIRPQVSGLIR
EIAFKEGGEIKKGDLLYQIEDAPYIAAVEQAKAAISKAEASVPSAESNLDRYQRLVGSGATQIEFETAKTTLLQAKAEVE
SAKAALSAAQIDLDHTRIIAPFDGIIDQTAYNVGNVVSANQTTALTTIRQLDPIYISLTESSTNLLKLRDAMAAGDIKGA
ANVVFHLILEDGKEYNQQGKLDMSKQVVSETTGTFIIRVLFPNPDRVVLPGMYVRATVELGAENGYALPQLATSRDANGR
LTAQFVSAEGKVETRAFENSSPSNNSWLVTEGIKDGDQLIVSGLQSITSGMPVKPVPMKITDNGVVVAAEQPAAGDAQKP
AAK

Sequences:

>Translated_403_residues
MIRRALLISSALAFGALLAACSDNASKPAGNAGAAAAQQASPVGVITLNKGSFPVTTILPGRAETFQTADIRPQVSGLIR
EIAFKEGGEIKKGDLLYQIEDAPYIAAVEQAKAAISKAEASVPSAESNLDRYQRLVGSGATQIEFETAKTTLLQAKAEVE
SAKAALSAAQIDLDHTRIIAPFDGIIDQTAYNVGNVVSANQTTALTTIRQLDPIYISLTESSTNLLKLRDAMAAGDIKGA
ANVVFHLILEDGKEYNQQGKLDMSKQVVSETTGTFIIRVLFPNPDRVVLPGMYVRATVELGAENGYALPQLATSRDANGR
LTAQFVSAEGKVETRAFENSSPSNNSWLVTEGIKDGDQLIVSGLQSITSGMPVKPVPMKITDNGVVVAAEQPAAGDAQKP
AAK
>Mature_403_residues
MIRRALLISSALAFGALLAACSDNASKPAGNAGAAAAQQASPVGVITLNKGSFPVTTILPGRAETFQTADIRPQVSGLIR
EIAFKEGGEIKKGDLLYQIEDAPYIAAVEQAKAAISKAEASVPSAESNLDRYQRLVGSGATQIEFETAKTTLLQAKAEVE
SAKAALSAAQIDLDHTRIIAPFDGIIDQTAYNVGNVVSANQTTALTTIRQLDPIYISLTESSTNLLKLRDAMAAGDIKGA
ANVVFHLILEDGKEYNQQGKLDMSKQVVSETTGTFIIRVLFPNPDRVVLPGMYVRATVELGAENGYALPQLATSRDANGR
LTAQFVSAEGKVETRAFENSSPSNNSWLVTEGIKDGDQLIVSGLQSITSGMPVKPVPMKITDNGVVVAAEQPAAGDAQKP
AAK

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=379, Percent_Identity=38.5224274406332, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786668, Length=392, Percent_Identity=39.030612244898, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=37.2340425531915, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082036, Length=311, Percent_Identity=26.3665594855305, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081951, Length=125, Percent_Identity=32, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42411; Mature: 42411

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRALLISSALAFGALLAACSDNASKPAGNAGAAAAQQASPVGVITLNKGSFPVTTILP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEC
GRAETFQTADIRPQVSGLIREIAFKEGGEIKKGDLLYQIEDAPYIAAVEQAKAAISKAEA
CCCCCEECCCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHC
SVPSAESNLDRYQRLVGSGATQIEFETAKTTLLQAKAEVESAKAALSAAQIDLDHTRIIA
CCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
PFDGIIDQTAYNVGNVVSANQTTALTTIRQLDPIYISLTESSTNLLKLRDAMAAGDIKGA
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCHH
ANVVFHLILEDGKEYNQQGKLDMSKQVVSETTGTFIIRVLFPNPDRVVLPGMYVRATVEL
HHHHHHHHHHCCHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEECCEEEEEEEEE
GAENGYALPQLATSRDANGRLTAQFVSAEGKVETRAFENSSPSNNSWLVTEGIKDGDQLI
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHH
VSGLQSITSGMPVKPVPMKITDNGVVVAAEQPAAGDAQKPAAK
HHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIRRALLISSALAFGALLAACSDNASKPAGNAGAAAAQQASPVGVITLNKGSFPVTTILP
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCEEEEEC
GRAETFQTADIRPQVSGLIREIAFKEGGEIKKGDLLYQIEDAPYIAAVEQAKAAISKAEA
CCCCCEECCCCCHHHHHHHHHHHHHCCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHC
SVPSAESNLDRYQRLVGSGATQIEFETAKTTLLQAKAEVESAKAALSAAQIDLDHTRIIA
CCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
PFDGIIDQTAYNVGNVVSANQTTALTTIRQLDPIYISLTESSTNLLKLRDAMAAGDIKGA
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCHH
ANVVFHLILEDGKEYNQQGKLDMSKQVVSETTGTFIIRVLFPNPDRVVLPGMYVRATVEL
HHHHHHHHHHCCHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCEEEECCEEEEEEEEE
GAENGYALPQLATSRDANGRLTAQFVSAEGKVETRAFENSSPSNNSWLVTEGIKDGDQLI
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHH
VSGLQSITSGMPVKPVPMKITDNGVVVAAEQPAAGDAQKPAAK
HHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]