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The map label for this gene is rluB [H]

Identifier: 209550726

GI number: 209550726

Start: 3229747

End: 3230553

Strand: Direct

Name: rluB [H]

Synonym: Rleg2_3150

Alternate gene names: 209550726

Gene position: 3229747-3230553 (Clockwise)

Preceding gene: 209550725

Following gene: 209550727

Centisome position: 71.17

GC content: 65.06

Gene sequence:

>807_bases
ATGAGGGAACGGCGCCCATCGCACAAGACCCGCGAGCGGACAAAGCCCGCCGCTGGCGGCGCAACCAAGCGGGTCACCCT
CCCCCGCGCCCTTTCCAAACTTGGTTATTGCTCCAGAACCCAGGCCGAACGGCTGATCGCCGAAAGTCGCGTTGCCGTCG
ACGGCCGCACCATCAGCGATATCTCCGCCTGGGTCGACCTCGCGACGGCCAGGATCAGCGTCGACGGCCTCGTCATTGCC
GCCGAAGCGAAAATCTATCTGATGCTCAACAAGCCCCGCGGGCTCGTCACCACCCGCGACGATCCCGAAGGCAGGCCGAC
GGTCTATGATTGCCTGAGGGAGTTCGACATCCCGCATCTCTCTCCGGTCGGCCGGCTCGACAAGGCGAGCGAAGGCCTGC
TGCTGTTTACCAATGACACGGAATTCGCCCAGATCCTGCTCGATCCGGTCACCCATGTGACGAAGACCTATCATGTCCAG
ATCGACCGGATCATGGACGATGAGGGCATTGCCGCCATGACATCGGGCATCCGCCACGACGGCGAACTGCTGACGGCAAC
CGCGGCGCGGCGCCTGCGCCAGGGCGACCGGAATTCATGGATCGAGGTCGAGCTCGACGAGGGCCGTAACCGCCAGATCC
GCCGCATGCTGGAGGCGCTCGGGACCGAATGCCTGCGCCTCGTGCGCGTTGCGATCGGCGGGCTCGAGCTCGGCGAACTG
CCGAAAGGCGCCGTGCGCGCGCTGACCGAAGCGGAATTGCAGTCGCTGCGGCGGAAAACCGGCATCGAGAAGGCGAGACG
CAATTGA

Upstream 100 bases:

>100_bases
GTTTCTAACGCGTCTCTTCTCCCCGCTTTTTCTTCGCCTTGCTGGCATCCTTGCTCCGTTCATGCCAAGAGAAGGCATCG
AGGCAGCATAAGGACCGCAG

Downstream 100 bases:

>100_bases
CTGATGAAAGGGATATGGCGCCGCACGACTTCTGGCAGGAGGTTCATTCGCCCGGCACCTTTGCCGTCGACGGCGAATTC
GCCTCCTTCTACGTCGCCGT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRERRPSHKTRERTKPAAGGATKRVTLPRALSKLGYCSRTQAERLIAESRVAVDGRTISDISAWVDLATARISVDGLVIA
AEAKIYLMLNKPRGLVTTRDDPEGRPTVYDCLREFDIPHLSPVGRLDKASEGLLLFTNDTEFAQILLDPVTHVTKTYHVQ
IDRIMDDEGIAAMTSGIRHDGELLTATAARRLRQGDRNSWIEVELDEGRNRQIRRMLEALGTECLRLVRVAIGGLELGEL
PKGAVRALTEAELQSLRRKTGIEKARRN

Sequences:

>Translated_268_residues
MRERRPSHKTRERTKPAAGGATKRVTLPRALSKLGYCSRTQAERLIAESRVAVDGRTISDISAWVDLATARISVDGLVIA
AEAKIYLMLNKPRGLVTTRDDPEGRPTVYDCLREFDIPHLSPVGRLDKASEGLLLFTNDTEFAQILLDPVTHVTKTYHVQ
IDRIMDDEGIAAMTSGIRHDGELLTATAARRLRQGDRNSWIEVELDEGRNRQIRRMLEALGTECLRLVRVAIGGLELGEL
PKGAVRALTEAELQSLRRKTGIEKARRN
>Mature_268_residues
MRERRPSHKTRERTKPAAGGATKRVTLPRALSKLGYCSRTQAERLIAESRVAVDGRTISDISAWVDLATARISVDGLVIA
AEAKIYLMLNKPRGLVTTRDDPEGRPTVYDCLREFDIPHLSPVGRLDKASEGLLLFTNDTEFAQILLDPVTHVTKTYHVQ
IDRIMDDEGIAAMTSGIRHDGELLTATAARRLRQGDRNSWIEVELDEGRNRQIRRMLEALGTECLRLVRVAIGGLELGEL
PKGAVRALTEAELQSLRRKTGIEKARRN

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=259, Percent_Identity=35.5212355212355, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=32.4894514767932, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788510, Length=223, Percent_Identity=33.6322869955157, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=33.8888888888889, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 29897; Mature: 29897

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERRPSHKTRERTKPAAGGATKRVTLPRALSKLGYCSRTQAERLIAESRVAVDGRTISD
CCCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCHHH
ISAWVDLATARISVDGLVIAAEAKIYLMLNKPRGLVTTRDDPEGRPTVYDCLREFDIPHL
HHHHHHHHHHEEEECCEEEEECCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHCCCCCC
SPVGRLDKASEGLLLFTNDTEFAQILLDPVTHVTKTYHVQIDRIMDDEGIAAMTSGIRHD
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEHCCCCCHHHHHHCCCCC
GELLTATAARRLRQGDRNSWIEVELDEGRNRQIRRMLEALGTECLRLVRVAIGGLELGEL
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PKGAVRALTEAELQSLRRKTGIEKARRN
CHHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MRERRPSHKTRERTKPAAGGATKRVTLPRALSKLGYCSRTQAERLIAESRVAVDGRTISD
CCCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHEECCCCHHH
ISAWVDLATARISVDGLVIAAEAKIYLMLNKPRGLVTTRDDPEGRPTVYDCLREFDIPHL
HHHHHHHHHHEEEECCEEEEECCEEEEEEECCCCEEECCCCCCCCCHHHHHHHHCCCCCC
SPVGRLDKASEGLLLFTNDTEFAQILLDPVTHVTKTYHVQIDRIMDDEGIAAMTSGIRHD
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHEEEEEEEEHCCCCCHHHHHHCCCCC
GELLTATAARRLRQGDRNSWIEVELDEGRNRQIRRMLEALGTECLRLVRVAIGGLELGEL
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PKGAVRALTEAELQSLRRKTGIEKARRN
CHHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]