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The map label for this gene is noeJ [H]

Identifier: 209550570

GI number: 209550570

Start: 3058498

End: 3059925

Strand: Direct

Name: noeJ [H]

Synonym: Rleg2_2994

Alternate gene names: 209550570

Gene position: 3058498-3059925 (Clockwise)

Preceding gene: 209550569

Following gene: 209550571

Centisome position: 67.4

GC content: 60.36

Gene sequence:

>1428_bases
ATGACGCAGAAAATCGTTCCCGTGATCATGGCCGGCGGCAAAGGCACGCGGCTCTGGCCGCTTTCCCGTGCCACCGCACC
GAAGCAGTTCATCCAGTTCGTCGGCAACAAGACGCTGTTTCAGGAGACGCTCGAACGCGTTTCCAACCCGGAGCTCTATG
AAGCCCCGATCATCGTCACCAATGAGGAATTTCGCTTCCTGGTCGCCGAGCAGGCCCGCACTCTGGGCGTCCCGCTCGCC
GCGGTGCTGCTTGAGCCGGTCGCCCGCAACACCGCCGCAGCGGTGGCCGCCGCCGCTACCCTTGCCGCCGAGCTTTTCGG
CAAGAACACCATCATCCAGATGCTCGCCTCGGATCACGAGATCCTCGCCGACAAGAGCTATTTCGATTGCATCCGCATCG
CCCGGAATGCTGCAGCCGACGGCAAGCTCGTTACTTTCGGCATCAGCCCCACCGAGCCGGCAACCGGCTACGGCTATATC
GAAATCGGCGATGCGCTTGAAAACGGCGCCCACAAGGTCAGCTGTTTCGTGGAAAAACCGGCATTGGAGAAGGCCGAGCA
GATGCTTGCCGACGGCGGCTTCTATTGGAACTCCGGCATCTTCATGTTCCCGGTGGAGGAACTCATTGCCGAGCTGCAGG
AATATGCACCCGACGTGCTGAAGGCGGCGAGCAAAGCCGTTTCCAAGGCAAGCCGCGACCTCGACTTTACCCGCCTCGAC
GCCGACCATTTCGCCAAGAGCCCCGATATCTCGATCGACTATGCGATCATGGAAAAGACCTCCAAGGCCGCTGTCGTCCC
CTCGCCGTTCAAATGGTCCGACATGGGAAGCTGGGATGCCGTTTGGAAATCAGGCGTGCGTGACGACAACGGCAATGTCG
CCGCCGCAAACACCACCGTCGTCAACACACGCAATTCGCTGGTCATGACCCACGGCGTGCATCTTGCCGTCCAAGGCATG
GAGGATGTGGCCGTCATCGCCAGCGAGGACGCCGTCTATGTCGGACCGCTCAAAGACAGCCAGAATGTCGGGCAATTGGT
CAAGATGCTGGCCTCCTCTTCCGCCACGGCGAAATTCACCGAAACCCATCCGACATCCTATCGTCCCTGGGGCGGCTATA
CCTCGATCTTCAACGGCGATCGCTTCCAGGTGAAGCGTATCTTCGTGACGCCGGGCAAGAAGCTCTCGCTGCAGAAACAC
CACCATCGCTCCGAACACTGGGTCGTCGTCAAGGGAACGGCCGAGGTGACGGTCGGCGAGACCGTGCGGATGCTGCGCGA
AAACGAGAGCGTCTATATTCCGCTCGGCGAAGTCCATCGTCTCGCCAACCCTGGCAAGATCCTCCTCGAACTGATCGAGG
TGCAGACGGGCTCCTATCTCGGCGAGGACGACATCATCCGTATCGTCGATGAGTTCGGAAGAACGTGA

Upstream 100 bases:

>100_bases
GCCGCCCTGGATCTGCTGAAGACAGGGCTCACCCGAATAACGGACTGGGCAAACGCCCGCTAAACATGCCACGAACAAGC
TTTTTTCAAGGAACCCGCCT

Downstream 100 bases:

>100_bases
CGTCGCAGCCGAACCGCCTGGCCAGGCCCACACAATGCTTTTCCGGCGCGACCCGCCATCGCTGTTGAATTCGCTATCGG
ACTTCTCGTAGAATGGACGC

Product: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTQKIVPVIMAGGKGTRLWPLSRATAPKQFIQFVGNKTLFQETLERVSNPELYEAPIIVTNEEFRFLVAEQARTLGVPLA
AVLLEPVARNTAAAVAAAATLAAELFGKNTIIQMLASDHEILADKSYFDCIRIARNAAADGKLVTFGISPTEPATGYGYI
EIGDALENGAHKVSCFVEKPALEKAEQMLADGGFYWNSGIFMFPVEELIAELQEYAPDVLKAASKAVSKASRDLDFTRLD
ADHFAKSPDISIDYAIMEKTSKAAVVPSPFKWSDMGSWDAVWKSGVRDDNGNVAAANTTVVNTRNSLVMTHGVHLAVQGM
EDVAVIASEDAVYVGPLKDSQNVGQLVKMLASSSATAKFTETHPTSYRPWGGYTSIFNGDRFQVKRIFVTPGKKLSLQKH
HHRSEHWVVVKGTAEVTVGETVRMLRENESVYIPLGEVHRLANPGKILLELIEVQTGSYLGEDDIIRIVDEFGRT

Sequences:

>Translated_475_residues
MTQKIVPVIMAGGKGTRLWPLSRATAPKQFIQFVGNKTLFQETLERVSNPELYEAPIIVTNEEFRFLVAEQARTLGVPLA
AVLLEPVARNTAAAVAAAATLAAELFGKNTIIQMLASDHEILADKSYFDCIRIARNAAADGKLVTFGISPTEPATGYGYI
EIGDALENGAHKVSCFVEKPALEKAEQMLADGGFYWNSGIFMFPVEELIAELQEYAPDVLKAASKAVSKASRDLDFTRLD
ADHFAKSPDISIDYAIMEKTSKAAVVPSPFKWSDMGSWDAVWKSGVRDDNGNVAAANTTVVNTRNSLVMTHGVHLAVQGM
EDVAVIASEDAVYVGPLKDSQNVGQLVKMLASSSATAKFTETHPTSYRPWGGYTSIFNGDRFQVKRIFVTPGKKLSLQKH
HHRSEHWVVVKGTAEVTVGETVRMLRENESVYIPLGEVHRLANPGKILLELIEVQTGSYLGEDDIIRIVDEFGRT
>Mature_474_residues
TQKIVPVIMAGGKGTRLWPLSRATAPKQFIQFVGNKTLFQETLERVSNPELYEAPIIVTNEEFRFLVAEQARTLGVPLAA
VLLEPVARNTAAAVAAAATLAAELFGKNTIIQMLASDHEILADKSYFDCIRIARNAAADGKLVTFGISPTEPATGYGYIE
IGDALENGAHKVSCFVEKPALEKAEQMLADGGFYWNSGIFMFPVEELIAELQEYAPDVLKAASKAVSKASRDLDFTRLDA
DHFAKSPDISIDYAIMEKTSKAAVVPSPFKWSDMGSWDAVWKSGVRDDNGNVAAANTTVVNTRNSLVMTHGVHLAVQGME
DVAVIASEDAVYVGPLKDSQNVGQLVKMLASSSATAKFTETHPTSYRPWGGYTSIFNGDRFQVKRIFVTPGKKLSLQKHH
HRSEHWVVVKGTAEVTVGETVRMLRENESVYIPLGEVHRLANPGKILLELIEVQTGSYLGEDDIIRIVDEFGRT

Specific function: Involved In The Biosynthesis Of The Capsular Polysaccharide Colanic Acid. [C]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=476, Percent_Identity=45.1680672268908, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 51931; Mature: 51800

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKIVPVIMAGGKGTRLWPLSRATAPKQFIQFVGNKTLFQETLERVSNPELYEAPIIVT
CCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCEEEE
NEEFRFLVAEQARTLGVPLAAVLLEPVARNTAAAVAAAATLAAELFGKNTIIQMLASDHE
CCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCH
ILADKSYFDCIRIARNAAADGKLVTFGISPTEPATGYGYIEIGDALENGAHKVSCFVEKP
HHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECHHHCCCCEEEEEEEECH
ALEKAEQMLADGGFYWNSGIFMFPVEELIAELQEYAPDVLKAASKAVSKASRDLDFTRLD
HHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEC
ADHFAKSPDISIDYAIMEKTSKAAVVPSPFKWSDMGSWDAVWKSGVRDDNGNVAAANTTV
HHHHCCCCCCEEEEHEECCCCCCEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECEEE
VNTRNSLVMTHGVHLAVQGMEDVAVIASEDAVYVGPLKDSQNVGQLVKMLASSSATAKFT
EECCCCEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
ETHPTSYRPWGGYTSIFNGDRFQVKRIFVTPGKKLSLQKHHHRSEHWVVVKGTAEVTVGE
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCCCCEEEEECCCEEEHHH
TVRMLRENESVYIPLGEVHRLANPGKILLELIEVQTGSYLGEDDIIRIVDEFGRT
HHHHHHCCCCEEEEHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TQKIVPVIMAGGKGTRLWPLSRATAPKQFIQFVGNKTLFQETLERVSNPELYEAPIIVT
CCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCEEEE
NEEFRFLVAEQARTLGVPLAAVLLEPVARNTAAAVAAAATLAAELFGKNTIIQMLASDHE
CCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCH
ILADKSYFDCIRIARNAAADGKLVTFGISPTEPATGYGYIEIGDALENGAHKVSCFVEKP
HHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEECHHHCCCCEEEEEEEECH
ALEKAEQMLADGGFYWNSGIFMFPVEELIAELQEYAPDVLKAASKAVSKASRDLDFTRLD
HHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEC
ADHFAKSPDISIDYAIMEKTSKAAVVPSPFKWSDMGSWDAVWKSGVRDDNGNVAAANTTV
HHHHCCCCCCEEEEHEECCCCCCEECCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECEEE
VNTRNSLVMTHGVHLAVQGMEDVAVIASEDAVYVGPLKDSQNVGQLVKMLASSSATAKFT
EECCCCEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEE
ETHPTSYRPWGGYTSIFNGDRFQVKRIFVTPGKKLSLQKHHHRSEHWVVVKGTAEVTVGE
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCCCCEEEEECCCEEEHHH
TVRMLRENESVYIPLGEVHRLANPGKILLELIEVQTGSYLGEDDIIRIVDEFGRT
HHHHHHCCCCEEEEHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]