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The map label for this gene is xylF [H]

Identifier: 209550502

GI number: 209550502

Start: 2982894

End: 2983934

Strand: Direct

Name: xylF [H]

Synonym: Rleg2_2925

Alternate gene names: 209550502

Gene position: 2982894-2983934 (Clockwise)

Preceding gene: 209550500

Following gene: 209550503

Centisome position: 65.73

GC content: 63.11

Gene sequence:

>1041_bases
ATGAAGTCTATTTTTAAATTGATGGCAGGCGCGGCCATTCTCGTTTCCGTGCATACCGCGGCCATGGCTGCCGATCTCGT
CGTCGGCGTTTCCTGGTCGAATTTCCAGGAAGAGCGCTGGAAGACCGATGAAGCCGCCATCAAGAAGGCTCTCGAAGCCA
AGGGCGCCAAGTATATTTCCGCCGACGCGCAGTCCTCAGCCGCAAAGCAGCTGACCGACGTCGAATCGCTGATCTCGCAG
GGCGCCAACGCGCTGATCATTCTCGCCCAGGATTCCGACGCCATCGGCCCGGCGATCGAAAAGGCCGCTGCCGAAGGCAT
CCCGGTCGTCGGCTACGACCGCCTGATCGAAAACCCGGCTGCCTTCTACATCACCTTCGACAACAAGGAAGTCGGCCGCC
TGCAGGCCGAAGGCGTCTTCAAGGCCAAGCCGGAAGGCAACTACGTCTTCATCAAGGGCTCATCCTCAGACCCGAACGCC
GACTTCCTGTTCTCCGGCCAGCAGGAAGTGCTGAAGGCTGCGATCGACGCCGGCAAGATCAAGAATGTCGGCGAGGCCTA
TACCGACGGCTGGCTGCCGGAGAACGCCCAGCGCAACATGGAGCAGTTCCTGACCGCCAACAACAACAAGGTTGACGCCG
TCGTCGCCTCCAATGACGGCACGGCCGGCGGCGCGATCGCAGCGCTCGACGCCCAGGGCCTCGCCGGCTCCGTTCCGGTC
TCCGGCCAGGACGGCGACAAGGCGGCGCTGAACCGCATCGCTCTCGGCACGCAGACGGTTTCCGTCTGGAAGGACAGCCG
CGAACTCGGCAAGCGCGCAGCCGAAATCGCTCTCGACCTCGCCGGCGGCAAGGACATGAGCAAGGTCGACGGCGCCCAGG
CTTTCAAGGGCGGCCCGAAGGGCGTGGAAATGCAGTCGGTTTTCCTGAAGCCGCTGGCGATCTCCAAGGACAATCTGAAC
GTCGTCATCGACGCCGGCTGGATCTCCAAGGCTGAAGCCTGCCAGGGCGTCAAGGCCGGCACCGTCGCCGCCTGCAAGTA
A

Upstream 100 bases:

>100_bases
TCGACTGTCGAGAAAATAATCCGAATTTCCCTCGACAGCTTTTCGCGGTGGCCGGAGGAAGCATGGGCTGGGCCGGTCGC
AACTCACTCGGGAGGACAGT

Downstream 100 bases:

>100_bases
GCAAGCTTCGAACGAAATTGCCGGCGCCGCAGCGGAAAGACCGTTGCGGCGCCGGATGCGGATGAGAACTCCGACCACAG
AGCTTTCTCCGCCTCGCCGC

Product: D-xylose ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKSIFKLMAGAAILVSVHTAAMAADLVVGVSWSNFQEERWKTDEAAIKKALEAKGAKYISADAQSSAAKQLTDVESLISQ
GANALIILAQDSDAIGPAIEKAAAEGIPVVGYDRLIENPAAFYITFDNKEVGRLQAEGVFKAKPEGNYVFIKGSSSDPNA
DFLFSGQQEVLKAAIDAGKIKNVGEAYTDGWLPENAQRNMEQFLTANNNKVDAVVASNDGTAGGAIAALDAQGLAGSVPV
SGQDGDKAALNRIALGTQTVSVWKDSRELGKRAAEIALDLAGGKDMSKVDGAQAFKGGPKGVEMQSVFLKPLAISKDNLN
VVIDAGWISKAEACQGVKAGTVAACK

Sequences:

>Translated_346_residues
MKSIFKLMAGAAILVSVHTAAMAADLVVGVSWSNFQEERWKTDEAAIKKALEAKGAKYISADAQSSAAKQLTDVESLISQ
GANALIILAQDSDAIGPAIEKAAAEGIPVVGYDRLIENPAAFYITFDNKEVGRLQAEGVFKAKPEGNYVFIKGSSSDPNA
DFLFSGQQEVLKAAIDAGKIKNVGEAYTDGWLPENAQRNMEQFLTANNNKVDAVVASNDGTAGGAIAALDAQGLAGSVPV
SGQDGDKAALNRIALGTQTVSVWKDSRELGKRAAEIALDLAGGKDMSKVDGAQAFKGGPKGVEMQSVFLKPLAISKDNLN
VVIDAGWISKAEACQGVKAGTVAACK
>Mature_346_residues
MKSIFKLMAGAAILVSVHTAAMAADLVVGVSWSNFQEERWKTDEAAIKKALEAKGAKYISADAQSSAAKQLTDVESLISQ
GANALIILAQDSDAIGPAIEKAAAEGIPVVGYDRLIENPAAFYITFDNKEVGRLQAEGVFKAKPEGNYVFIKGSSSDPNA
DFLFSGQQEVLKAAIDAGKIKNVGEAYTDGWLPENAQRNMEQFLTANNNKVDAVVASNDGTAGGAIAALDAQGLAGSVPV
SGQDGDKAALNRIALGTQTVSVWKDSRELGKRAAEIALDLAGGKDMSKVDGAQAFKGGPKGVEMQSVFLKPLAISKDNLN
VVIDAGWISKAEACQGVKAGTVAACK

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=317, Percent_Identity=43.217665615142, Blast_Score=244, Evalue=5e-66,
Organism=Escherichia coli, GI1788473, Length=335, Percent_Identity=28.0597014925373, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36034; Mature: 36034

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIFKLMAGAAILVSVHTAAMAADLVVGVSWSNFQEERWKTDEAAIKKALEAKGAKYIS
CHHHHHHHHCHHHEEHHHHHHHHHHHEEECCCCCCHHHHCCCHHHHHHHHHHHCCCEEEE
ADAQSSAAKQLTDVESLISQGANALIILAQDSDAIGPAIEKAAAEGIPVVGYDRLIENPA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCCE
AFYITFDNKEVGRLQAEGVFKAKPEGNYVFIKGSSSDPNADFLFSGQQEVLKAAIDAGKI
EEEEEECCCCCCCEEECCEEEECCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCC
KNVGEAYTDGWLPENAQRNMEQFLTANNNKVDAVVASNDGTAGGAIAALDAQGLAGSVPV
CCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
SGQDGDKAALNRIALGTQTVSVWKDSRELGKRAAEIALDLAGGKDMSKVDGAQAFKGGPK
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCHHHHCCCCC
GVEMQSVFLKPLAISKDNLNVVIDAGWISKAEACQGVKAGTVAACK
CCEEHHHHHCCEEECCCCCEEEEECCCCCHHHHHCCCCCCCEEECC
>Mature Secondary Structure
MKSIFKLMAGAAILVSVHTAAMAADLVVGVSWSNFQEERWKTDEAAIKKALEAKGAKYIS
CHHHHHHHHCHHHEEHHHHHHHHHHHEEECCCCCCHHHHCCCHHHHHHHHHHHCCCEEEE
ADAQSSAAKQLTDVESLISQGANALIILAQDSDAIGPAIEKAAAEGIPVVGYDRLIENPA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHCCCE
AFYITFDNKEVGRLQAEGVFKAKPEGNYVFIKGSSSDPNADFLFSGQQEVLKAAIDAGKI
EEEEEECCCCCCCEEECCEEEECCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHCCC
KNVGEAYTDGWLPENAQRNMEQFLTANNNKVDAVVASNDGTAGGAIAALDAQGLAGSVPV
CCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCC
SGQDGDKAALNRIALGTQTVSVWKDSRELGKRAAEIALDLAGGKDMSKVDGAQAFKGGPK
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCHHHHCCCCC
GVEMQSVFLKPLAISKDNLNVVIDAGWISKAEACQGVKAGTVAACK
CCEEHHHHHCCEEECCCCCEEEEECCCCCHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]