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The map label for this gene is nepI [H]

Identifier: 209550475

GI number: 209550475

Start: 2950360

End: 2951568

Strand: Direct

Name: nepI [H]

Synonym: Rleg2_2898

Alternate gene names: 209550475

Gene position: 2950360-2951568 (Clockwise)

Preceding gene: 209550474

Following gene: 209550478

Centisome position: 65.02

GC content: 61.62

Gene sequence:

>1209_bases
ATGACGGACTCAACATCACAGTTCGACGGTGTCGCAATTGGCCTGGAGACGGATGAACCATCCGCGTGGAACACCGCGAC
CTGGTTTGCCGTTGTTTCGATGGCAGCCACCAGCTTTGCGCTGGTGTCCGCTGAGTTCCTGCCGGCGGGCCTGTTGACGC
CGATGGCGCGCGATCTCGGCATTTCCGAGGGAACGGCCGGACAAGTCGTCACCGCCACCGCTTCTGTCGGCGCCGTGGCG
GCCTTGTTGAGCAACGTCCTCATCGGCCGACTGAACCGCAAAACCGTGCTGGTCAGCCTCAGTGCACTGGCGATCGGCTC
CAATATTCTGGCATCAGTGGCGACTGATTTTTGGCTGTTGCTGTTGGGTCGGGCCGGGTTGGGCATCGCGCTTAGTGGCT
TTTGGGCGCTTTCAGTAGCCGTCGTGGCGAGGCTGGTCGGCGCCAATGCGACAGGTCGTGGCATGGCTATCGTCACGCTC
GGCGTTTCGCTCGCCACGATAGCTGCACCATCAATGGGTGCTTTGATCAGCGATTGGCTGGGATGGCGCAGCGCCATGGC
GATGACAGCCGGGCTTGCCGCGCTTGCCATGCTGCTGCAACTGCTCAGCTTGCCGACGCTCCCTGCAAGCACAAGCAACA
GTATCGCTGATGTTTTCCGGCTGACACGACGGGGCGGTATTCAACTGGGAATGCTTGCCATCCTTTTGTTGATGACCGGG
CATTTTGCCGGGTCGGTTTATGTGCGTCCTTTCCTCGAACAGGTAACACTGCTTGATACCCGATCAATCGCCCTGGCACT
GCTCGGATTTGGCATCGCCTCGGTGATCGGCAATGTTGCCGGTGGCCGAATGGCCGATGCCAGTATCCGGCTAGCACTCG
CTGTGACCGCCGCGCTGATGACGGTTGCAGCACTCGCTCTGGTGCTTTGGGGCGCCCATACCGGCATTGCGTTCGCCCTC
GTCGCACTTTGGGGCTTTGCCTTCGGAATGGCGCCGGTCGTGCTGCCGACCAATCTTTCCCGCGCAGCCCCCGACGCTCT
GGAGGCGGCGGGTAGCCTGATGGTCGTCTCTTTTCAGGTGGCCATCAGTATCGGCGCGGTTTTTGGCGGCTATATCGTCG
ACCACTACGGCGCCGTGGGACCATTGACTGTGACGGCGCTCCTGGCTGCGTCGACGATTGCCTTGGCGTTGCTGCAGCCC
CGCAGCTGA

Upstream 100 bases:

>100_bases
CGCACAAGTTCCGGACGAATGGATATAGATTTCGGATTTTAAGACACATATCATCCTGAAAACTTCGTCTATCAAATCTC
CGAACATAGGAGATATTTCA

Downstream 100 bases:

>100_bases
TCGTTGCTTTCTGCCTGCGGATCACGCACAGTCGAACTGAAGATGCTCTGGATAACAGGTCACCGGCAATCGCCGGCGAC
CTCCAATGCGTTCCGTCCGG

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDSTSQFDGVAIGLETDEPSAWNTATWFAVVSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATASVGAVA
ALLSNVLIGRLNRKTVLVSLSALAIGSNILASVATDFWLLLLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSMGALISDWLGWRSAMAMTAGLAALAMLLQLLSLPTLPASTSNSIADVFRLTRRGGIQLGMLAILLLMTG
HFAGSVYVRPFLEQVTLLDTRSIALALLGFGIASVIGNVAGGRMADASIRLALAVTAALMTVAALALVLWGAHTGIAFAL
VALWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVVSFQVAISIGAVFGGYIVDHYGAVGPLTVTALLAASTIALALLQP
RS

Sequences:

>Translated_402_residues
MTDSTSQFDGVAIGLETDEPSAWNTATWFAVVSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATASVGAVA
ALLSNVLIGRLNRKTVLVSLSALAIGSNILASVATDFWLLLLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSMGALISDWLGWRSAMAMTAGLAALAMLLQLLSLPTLPASTSNSIADVFRLTRRGGIQLGMLAILLLMTG
HFAGSVYVRPFLEQVTLLDTRSIALALLGFGIASVIGNVAGGRMADASIRLALAVTAALMTVAALALVLWGAHTGIAFAL
VALWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVVSFQVAISIGAVFGGYIVDHYGAVGPLTVTALLAASTIALALLQP
RS
>Mature_401_residues
TDSTSQFDGVAIGLETDEPSAWNTATWFAVVSMAATSFALVSAEFLPAGLLTPMARDLGISEGTAGQVVTATASVGAVAA
LLSNVLIGRLNRKTVLVSLSALAIGSNILASVATDFWLLLLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLG
VSLATIAAPSMGALISDWLGWRSAMAMTAGLAALAMLLQLLSLPTLPASTSNSIADVFRLTRRGGIQLGMLAILLLMTGH
FAGSVYVRPFLEQVTLLDTRSIALALLGFGIASVIGNVAGGRMADASIRLALAVTAALMTVAALALVLWGAHTGIAFALV
ALWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVVSFQVAISIGAVFGGYIVDHYGAVGPLTVTALLAASTIALALLQPR
S

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=373, Percent_Identity=37.8016085790885, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1787808, Length=350, Percent_Identity=25.7142857142857, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787947, Length=307, Percent_Identity=27.3615635179153, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786595, Length=344, Percent_Identity=23.546511627907, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40730; Mature: 40599

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSTSQFDGVAIGLETDEPSAWNTATWFAVVSMAATSFALVSAEFLPAGLLTPMARDLG
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATASVGAVAALLSNVLIGRLNRKTVLVSLSALAIGSNILASVATDFWLL
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSMGALISDWL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHH
GWRSAMAMTAGLAALAMLLQLLSLPTLPASTSNSIADVFRLTRRGGIQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEQVTLLDTRSIALALLGFGIASVIGNVAGGRMADASIRLALAVTAALM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TVAALALVLWGAHTGIAFALVALWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVVSFQV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCEEEEHHHH
AISIGAVFGGYIVDHYGAVGPLTVTALLAASTIALALLQPRS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDSTSQFDGVAIGLETDEPSAWNTATWFAVVSMAATSFALVSAEFLPAGLLTPMARDLG
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATASVGAVAALLSNVLIGRLNRKTVLVSLSALAIGSNILASVATDFWLL
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LLGRAGLGIALSGFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSMGALISDWL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHH
GWRSAMAMTAGLAALAMLLQLLSLPTLPASTSNSIADVFRLTRRGGIQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEQVTLLDTRSIALALLGFGIASVIGNVAGGRMADASIRLALAVTAALM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TVAALALVLWGAHTGIAFALVALWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVVSFQV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCEEEEHHHH
AISIGAVFGGYIVDHYGAVGPLTVTALLAASTIALALLQPRS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA