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The map label for this gene is carA [H]

Identifier: 209550286

GI number: 209550286

Start: 2747030

End: 2748241

Strand: Direct

Name: carA [H]

Synonym: Rleg2_2706

Alternate gene names: 209550286

Gene position: 2747030-2748241 (Clockwise)

Preceding gene: 209550284

Following gene: 209550287

Centisome position: 60.53

GC content: 63.04

Gene sequence:

>1212_bases
ATGAAGATGACCGCGACAGCACCCTGGACAATCGAAAAGCCGACCGCCCTCCTCGTTCTTGCCGACGGCACGGTCATCGA
AGGCAAGGGCATCGGCGCCACCGGCAAGGTCCAGGCCGAAGTGGTGTTCAACACGGCGCTGACCGGCTACGAGGAGATCC
TGACGGACCCCTCCTATCTCGGCCAGATCGTCACCTTCACCTTTCCGCATATCGGCAATATCGGCACCAATGATGAAGAC
ATCGAGGATCTGACGCCTGCTGCCCGCCACGGCGCCGTCGGCGTCATCTTCAAGGCCGACATCACCGACCCTTCGAACTA
TCGCGCCGCCAAGCATCTCGACCAATGGCTGAAGGCGCGCGGCGTCATCGGTCTCTGCGGCATCGACACGCGCGCGCTGA
CCGCCTGGATCCGCGAGAACGGCGCCCCGAATGCGGTCATCGCCCACGATCCGAACGGCGTCTTCGATATCGAGGCGCTG
AAGACCGAAGCCAAGGCCTGGAGCGGCCTCGAAGGTCTCGATCTCGCCAAGATCGCTTCATCGGGCCAGTCCTCGCAATG
GACCGAGACGCCGTGGGTCTGGAATGAAGGGTATGGCGAACTCGCCGCAGCGGATGCGAAATACCACGTCGTCTGCCTCG
ATTACGGCGTTAAGCGCAACATCCTGCGCCTGTTTGCCGGCCTCGACTGCAAGGTGACCGTCGTGCCGGCGGCGACCAGT
GCCGAAGACGTGCTCGCGATGCAGCCGGACGGCATCTTTCTGTCGAACGGCCCGGGCGACCCGGCGGCGACCGGCGAATA
TGCCGTGCCCGTAATCAAAACCCTTGTTAAGACCGACATCCCGGTCTTCGGCATCTGCCTCGGCCACCAGATGCTCGGCC
TTGCCCTCGGCGCCAAGACCGAGAAGATGCACCAGGGCCATCACGGCGCCAACCATCCCGTCAAGGACCATACGACCGGC
AAGGTCGAGATCGTCTCGATGAACCATGGCTTCGCGGTCGACTCGAAGTCGCTGCCCGATGGCGTTGAAGAGACTCATAT
TTCGCTTTTCGACGGCACCAATTGCGGCCTGCGCGTGCTCGGCAAGCAGGTCTTCTCCGTCCAGCACCATCCGGAAGCCT
CTCCCGGCCCGCAGGACAGCCACTATCTCTTCCGCCGCTTCATCAACATGGTGCGCGAGAAGAAGGGCGAACCGGCGCTC
GCCGAACGCTGA

Upstream 100 bases:

>100_bases
TTCGTGATCAGGCCTGAAGCGCGCGGCATTGTCGTGTCCACACGCCTGCGAGATCAAATGGCGACGAGCGCGGCAACGCG
CGCCGGCCGCCGGAAACGGG

Downstream 100 bases:

>100_bases
TCTTAGCCAACATCGATTTCAGGAAAGGCCCGTCGACCGGGCCTTTTTCTTTGCTCCGCATATTGACCTCAATTTAAGTT
GAGGAATTACAGAATTGCCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKMTATAPWTIEKPTALLVLADGTVIEGKGIGATGKVQAEVVFNTALTGYEEILTDPSYLGQIVTFTFPHIGNIGTNDED
IEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDQWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEAL
KTEAKAWSGLEGLDLAKIASSGQSSQWTETPWVWNEGYGELAAADAKYHVVCLDYGVKRNILRLFAGLDCKVTVVPAATS
AEDVLAMQPDGIFLSNGPGDPAATGEYAVPVIKTLVKTDIPVFGICLGHQMLGLALGAKTEKMHQGHHGANHPVKDHTTG
KVEIVSMNHGFAVDSKSLPDGVEETHISLFDGTNCGLRVLGKQVFSVQHHPEASPGPQDSHYLFRRFINMVREKKGEPAL
AER

Sequences:

>Translated_403_residues
MKMTATAPWTIEKPTALLVLADGTVIEGKGIGATGKVQAEVVFNTALTGYEEILTDPSYLGQIVTFTFPHIGNIGTNDED
IEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDQWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEAL
KTEAKAWSGLEGLDLAKIASSGQSSQWTETPWVWNEGYGELAAADAKYHVVCLDYGVKRNILRLFAGLDCKVTVVPAATS
AEDVLAMQPDGIFLSNGPGDPAATGEYAVPVIKTLVKTDIPVFGICLGHQMLGLALGAKTEKMHQGHHGANHPVKDHTTG
KVEIVSMNHGFAVDSKSLPDGVEETHISLFDGTNCGLRVLGKQVFSVQHHPEASPGPQDSHYLFRRFINMVREKKGEPAL
AER
>Mature_403_residues
MKMTATAPWTIEKPTALLVLADGTVIEGKGIGATGKVQAEVVFNTALTGYEEILTDPSYLGQIVTFTFPHIGNIGTNDED
IEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDQWLKARGVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEAL
KTEAKAWSGLEGLDLAKIASSGQSSQWTETPWVWNEGYGELAAADAKYHVVCLDYGVKRNILRLFAGLDCKVTVVPAATS
AEDVLAMQPDGIFLSNGPGDPAATGEYAVPVIKTLVKTDIPVFGICLGHQMLGLALGAKTEKMHQGHHGANHPVKDHTTG
KVEIVSMNHGFAVDSKSLPDGVEETHISLFDGTNCGLRVLGKQVFSVQHHPEASPGPQDSHYLFRRFINMVREKKGEPAL
AER

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=36.9791666666667, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI21361331, Length=419, Percent_Identity=32.9355608591885, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI169790915, Length=419, Percent_Identity=32.9355608591885, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1786215, Length=384, Percent_Identity=50.5208333333333, Blast_Score=373, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI193204318, Length=391, Percent_Identity=36.8286445012788, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6324878, Length=393, Percent_Identity=36.3867684478371, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=33.1658291457286, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24642586, Length=392, Percent_Identity=36.734693877551, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI45555749, Length=392, Percent_Identity=36.734693877551, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43242; Mature: 43242

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTATAPWTIEKPTALLVLADGTVIEGKGIGATGKVQAEVVFNTALTGYEEILTDPSYL
CCEECCCCEEECCCEEEEEEECCEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHCCHHHH
GQIVTFTFPHIGNIGTNDEDIEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDQWLKAR
CEEEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
GVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKTEAKAWSGLEGLDLAKIAS
CCEEEECCCHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCHHHHHC
SGQSSQWTETPWVWNEGYGELAAADAKYHVVCLDYGVKRNILRLFAGLDCKVTVVPAATS
CCCCCCCCCCCCEEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCC
AEDVLAMQPDGIFLSNGPGDPAATGEYAVPVIKTLVKTDIPVFGICLGHQMLGLALGAKT
CCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCH
EKMHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDSKSLPDGVEETHISLFDGTNCGLRVL
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCEEEEEECCCCCHHHH
GKQVFSVQHHPEASPGPQDSHYLFRRFINMVREKKGEPALAER
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKMTATAPWTIEKPTALLVLADGTVIEGKGIGATGKVQAEVVFNTALTGYEEILTDPSYL
CCEECCCCEEECCCEEEEEEECCEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHCCHHHH
GQIVTFTFPHIGNIGTNDEDIEDLTPAARHGAVGVIFKADITDPSNYRAAKHLDQWLKAR
CEEEEEECCCCCCCCCCCCCHHHCCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHC
GVIGLCGIDTRALTAWIRENGAPNAVIAHDPNGVFDIEALKTEAKAWSGLEGLDLAKIAS
CCEEEECCCHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCCHHHHHC
SGQSSQWTETPWVWNEGYGELAAADAKYHVVCLDYGVKRNILRLFAGLDCKVTVVPAATS
CCCCCCCCCCCCEEECCCCCEEECCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEECCCC
AEDVLAMQPDGIFLSNGPGDPAATGEYAVPVIKTLVKTDIPVFGICLGHQMLGLALGAKT
CCCEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCH
EKMHQGHHGANHPVKDHTTGKVEIVSMNHGFAVDSKSLPDGVEETHISLFDGTNCGLRVL
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCEEEEEECCCCCHHHH
GKQVFSVQHHPEASPGPQDSHYLFRRFINMVREKKGEPALAER
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]