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The map label for this gene is ilvE [H]

Identifier: 209550070

GI number: 209550070

Start: 2521261

End: 2522361

Strand: Direct

Name: ilvE [H]

Synonym: Rleg2_2487

Alternate gene names: 209550070

Gene position: 2521261-2522361 (Clockwise)

Preceding gene: 209550068

Following gene: 209550071

Centisome position: 55.56

GC content: 62.49

Gene sequence:

>1101_bases
ATGACCGTGACGTCTGCCTCTCCTGAAATCAAAATCGAACTCCACAAGTCTCCCGTCCCGGACGCCGATCGCATTCAGGC
ACTCGAGACGCCCGGCTTCGGCAAGCTCTTCACGGATCATATGGTCCTGGCACGATGGACTGCCGACAAGGGCTGGCACG
ATGCAAAGGTTACGCCCAGGCGACCCCTGGAACTCGATCCTGCGAGCGCCGTGCTGCACTATGCTCAGGAAATCTTCGAG
GGCATGAAAGCCTACAAGGCGGACGATGGCCGGATTCTGCTCTTTCGGCCTGAAGAGAACGCACGCCGCTTCGCGCAGTC
GGCGACCCGCATGGCGATGCCTCCCGTGCCCGAAGACCTCTTCCTGAAGGCGGTCGAAGAACTGGTCCGTGTCGACAAGG
CCTGGATTCCCACAGGCGACGCCAGCCTGTACCTTCGCCCCTTCATGTTCGCCAACGAGGCGTTTCTCGGCGTTCGTCCG
GCTCAGGAATATGTCTTCTGCATCATCGCCTCTCCGGTCGGCGCCTACTTCAAGGGCGGCGCAAAGGCGGTTTCCCTTTG
GGTCGAGACCGAATACACCCGTGCAGCCGCCGGCGGCACGGGTGCAGCAAAATGCGGCGGAAACTACGCAGCCAGCCTCG
TGGCGCAAGCCGAGGCGACCAAAAAGGGTTGCGATCAGGTGGTCTTCCTGGACGCCGCAGAGCATCGTTGGGTCGAAGAA
CTCGGCGGCATGAACGTCTTCTTCGTGATGAACGACGGATCAGTGGTGACCCCGCCCCTTGGCGGCACGATCCTGCCGGG
CATCACCCGGGCTTCCGTGATCGTGCTCACCGAAGAAAGGGGCTTACGCGTCGAGGAGCGCCCCTATTCGTTCGCGGAAT
GGCAAGACGACGCTGCCAGTGGCAGGCTCGTCGAAGCATTCGCTTGCGGCACTGCCGCAGTTCTGGCGGGCATCGGCCTG
GTTCGACATGCCGGCGGCGAGTTTCTGGTCGGAGACGGACAGACCGGCAAGTTGACCAGTGAACTGCGCCAGCAGCTCGT
CAGCCTGCAGAAGGGCGTAACGAACGATGAGCACGGCTGGACGCGCCTCATCCCGGCCTGA

Upstream 100 bases:

>100_bases
TCTGATCAAAGTCGAAACCGGCTGATTCAGACCCTTCCAAGACCAGCTCGGCGGCGGTAGTGTCGCCGCCACTATTCAAT
TTACTATCAAGGTGCCAGAA

Downstream 100 bases:

>100_bases
ATTCGGACGTTCCACGCGACTGGCGGCGTCAACACCGACAGGGGGGCGCCGCATGTGGCCCCCTCGGCACAGCGCTTGCC
CACCGTGCAGCAGAGATATC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTVTSASPEIKIELHKSPVPDADRIQALETPGFGKLFTDHMVLARWTADKGWHDAKVTPRRPLELDPASAVLHYAQEIFE
GMKAYKADDGRILLFRPEENARRFAQSATRMAMPPVPEDLFLKAVEELVRVDKAWIPTGDASLYLRPFMFANEAFLGVRP
AQEYVFCIIASPVGAYFKGGAKAVSLWVETEYTRAAAGGTGAAKCGGNYAASLVAQAEATKKGCDQVVFLDAAEHRWVEE
LGGMNVFFVMNDGSVVTPPLGGTILPGITRASVIVLTEERGLRVEERPYSFAEWQDDAASGRLVEAFACGTAAVLAGIGL
VRHAGGEFLVGDGQTGKLTSELRQQLVSLQKGVTNDEHGWTRLIPA

Sequences:

>Translated_366_residues
MTVTSASPEIKIELHKSPVPDADRIQALETPGFGKLFTDHMVLARWTADKGWHDAKVTPRRPLELDPASAVLHYAQEIFE
GMKAYKADDGRILLFRPEENARRFAQSATRMAMPPVPEDLFLKAVEELVRVDKAWIPTGDASLYLRPFMFANEAFLGVRP
AQEYVFCIIASPVGAYFKGGAKAVSLWVETEYTRAAAGGTGAAKCGGNYAASLVAQAEATKKGCDQVVFLDAAEHRWVEE
LGGMNVFFVMNDGSVVTPPLGGTILPGITRASVIVLTEERGLRVEERPYSFAEWQDDAASGRLVEAFACGTAAVLAGIGL
VRHAGGEFLVGDGQTGKLTSELRQQLVSLQKGVTNDEHGWTRLIPA
>Mature_365_residues
TVTSASPEIKIELHKSPVPDADRIQALETPGFGKLFTDHMVLARWTADKGWHDAKVTPRRPLELDPASAVLHYAQEIFEG
MKAYKADDGRILLFRPEENARRFAQSATRMAMPPVPEDLFLKAVEELVRVDKAWIPTGDASLYLRPFMFANEAFLGVRPA
QEYVFCIIASPVGAYFKGGAKAVSLWVETEYTRAAAGGTGAAKCGGNYAASLVAQAEATKKGCDQVVFLDAAEHRWVEEL
GGMNVFFVMNDGSVVTPPLGGTILPGITRASVIVLTEERGLRVEERPYSFAEWQDDAASGRLVEAFACGTAAVLAGIGLV
RHAGGEFLVGDGQTGKLTSELRQQLVSLQKGVTNDEHGWTRLIPA

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=340, Percent_Identity=37.6470588235294, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI296010904, Length=340, Percent_Identity=37.6470588235294, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI296010906, Length=340, Percent_Identity=37.6470588235294, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI50658084, Length=357, Percent_Identity=36.6946778711485, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI296010902, Length=340, Percent_Identity=33.2352941176471, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI296010900, Length=340, Percent_Identity=33.2352941176471, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI258614015, Length=289, Percent_Identity=35.9861591695502, Blast_Score=179, Evalue=3e-45,
Organism=Escherichia coli, GI48994963, Length=322, Percent_Identity=32.2981366459627, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=296, Percent_Identity=40.2027027027027, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17565728, Length=336, Percent_Identity=36.6071428571429, Blast_Score=198, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322608, Length=371, Percent_Identity=38.0053908355795, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322002, Length=367, Percent_Identity=37.0572207084469, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24641779, Length=373, Percent_Identity=38.3378016085791, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39708; Mature: 39576

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTSASPEIKIELHKSPVPDADRIQALETPGFGKLFTDHMVLARWTADKGWHDAKVTPR
CCCCCCCCEEEEEEECCCCCCHHHHEEECCCCCCHHHHHCEEEEEECCCCCCCCCCCCCC
RPLELDPASAVLHYAQEIFEGMKAYKADDGRILLFRPEENARRFAQSATRMAMPPVPEDL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHH
FLKAVEELVRVDKAWIPTGDASLYLRPFMFANEAFLGVRPAQEYVFCIIASPVGAYFKGG
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEECCCCCCEEEEEEECCHHHHHCCC
AKAVSLWVETEYTRAAAGGTGAAKCGGNYAASLVAQAEATKKGCDQVVFLDAAEHRWVEE
CEEEEEEEECCHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
LGGMNVFFVMNDGSVVTPPLGGTILPGITRASVIVLTEERGLRVEERPYSFAEWQDDAAS
HCCCEEEEEECCCCEECCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHCCCCCCC
GRLVEAFACGTAAVLAGIGLVRHAGGEFLVGDGQTGKLTSELRQQLVSLQKGVTNDEHGW
CCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCE
TRLIPA
EEECCC
>Mature Secondary Structure 
TVTSASPEIKIELHKSPVPDADRIQALETPGFGKLFTDHMVLARWTADKGWHDAKVTPR
CCCCCCCEEEEEEECCCCCCHHHHEEECCCCCCHHHHHCEEEEEECCCCCCCCCCCCCC
RPLELDPASAVLHYAQEIFEGMKAYKADDGRILLFRPEENARRFAQSATRMAMPPVPEDL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHH
FLKAVEELVRVDKAWIPTGDASLYLRPFMFANEAFLGVRPAQEYVFCIIASPVGAYFKGG
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEECCCCCCEEEEEEECCHHHHHCCC
AKAVSLWVETEYTRAAAGGTGAAKCGGNYAASLVAQAEATKKGCDQVVFLDAAEHRWVEE
CEEEEEEEECCHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHH
LGGMNVFFVMNDGSVVTPPLGGTILPGITRASVIVLTEERGLRVEERPYSFAEWQDDAAS
HCCCEEEEEECCCCEECCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCHHHCCCCCCC
GRLVEAFACGTAAVLAGIGLVRHAGGEFLVGDGQTGKLTSELRQQLVSLQKGVTNDEHGW
CCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCE
TRLIPA
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]