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The map label for this gene is recG [H]

Identifier: 209549324

GI number: 209549324

Start: 1765743

End: 1767848

Strand: Reverse

Name: recG [H]

Synonym: Rleg2_1728

Alternate gene names: 209549324

Gene position: 1767848-1765743 (Counterclockwise)

Preceding gene: 209549328

Following gene: 209549309

Centisome position: 38.96

GC content: 65.24

Gene sequence:

>2106_bases
ATGCGCCCCGCCATTCTCGATCCTCTGTTTTCTCCCATTTCGGGCCTTCCGGGCGTTGGCCCGAAGATCGCCGAACTGCT
GGTCAAGCTGCTCGGCCGCGAGACGCCGGAGGATTGCCGGGTCATCGATCTCATCTTCCACGCGCCGTTTTCGCTGATCG
ACCGGCGCAACCAGCCGGGGATCGCCCGCGCGCCGCAGGGCGCGATCGTGACGATCACCGCCCGTGTCGACCGGCACCAG
GTTCCGCCGCGCGGCAAGAGCAATGTCCCCTACCGCGTCTTCCTGCATGACGAGACCGGCGAGCTGACGCTGGTCTTTTT
CCGCGGCCAGGCGGCTTGGCTGGAAAAACAGCTGCCTGTCGACGCTGAGGTGACGGTCAGCGGCAAGGTCGACTGGTTCA
ACGGCCGCGCCTCGATGGTGCATCCCGATTATATCGTCAGGGCTGATGAAGCGGAGAACCTGCCGCTCGTCGAGCCGATC
TATCCGCTGACGGCGGGGCTTTCGCCGAAGACATTGCGCAAGATCATCGACGCCGCCCTGCCGCGGTTTCCCGAGCTGCC
GGAATGGATCGACCAGGCGCTGGCGCAGAAGCAGGGACTACCAACAATCCGCGACAGCTTCCACATGTTGCACGAGCCGC
GCGACCCGGCCGATATCGACGCGCAGGCCCCTGCCCGGCGGCGGCTTGCCTATGACGAATTCCTTGCCGGTCAGCTGTCG
CTGAGCCTGGTGCGCCAGAGGCTGCGCAAGGTGGCGGGCCAGCCGGTGCAAGCGACGGGCGAAGTCAGCGCCAAAATCCT
GAAATCCCTGCCCTTCTCGCTCACATCGAGCCAGAACGAGGCGATCGCCGAGGTTTTGAAGGACATGGCCGGCACCGAGC
GCATGCTGCGCCTGCTGCAGGGCGATGTCGGTTCCGGCAAGACGCTGGTGGCGCTGATGGCGATGGCAGCGGTGATCGAA
AGCGGCGGCCAGGCCGTGCTGATGGCACCGACCGAAATCCTGGCGCGCCAGCACCATGCGACAATCTCGAAATTCGCCGC
CTCCGCCGGGCTTGGCATCGAGATCCTGACCGGCCGCACCAAGGGGCGCGAGCGGGAGGAGATCCTCGACCGCATCGCCT
CGGGCGCCGCGCAAATCATTATCGGCACGCATGCGCTGTTCCAGGACAGCGTCGCTTACGCCAAGCTGATGCTGGCCGTC
GTCGACGAGCAACACCGTTTCGGTGTGCATCAGCGCCTGAGATTGACTGCCAAGGGCATCTCGCCGCACATGCTGGTGAT
GACGGCGACGCCGATCCCGCGCACGCTGGTGCTTGCCGCCTTCGGCGACATGGACGTTTCCAAACTCACCGAGAAGCCGG
CCGGCCGTAAGCCGATCCAGACGATCACCGTGCCGATGGAGCGGACAGGCGAAATCGTCGGGCGGCTGCAAAGCGCGCTT
GCCGAGGGCAAGAAGGCCTACTGGATTTGCCCGCTGGTCGAAGAATCCGAAGAACTCGACCTGATGTCGGCCGAGGAGCG
GCACGCCACGCTCGTTGCCGCGCTCGGCCCCGATATCGGCCTCATTCACGGCCGCATGAGCGGGCCGGAGAAGGATGCGG
CGATGATGGCGTTCAAGAACGGCGAGACCCGCCTCCTCGTCGCCACCACCGTCGTCGAGGTCGGCGTCGACGTGCCGGAT
GCGACGATCATGGTGATCGAACATGCCGAACGCTTCGGCCTGGCACAATTGCACCAGCTGCGCGGCCGCGTCGGGCGCGG
CGACGAAGCCTCGACCTGCATCCTGCTCTACAAGGGGCCGCTCGGCGAAACCGGCCATGCCAGGCTTTCGATCATGCGCG
AGACCGAAGATGGCTTCCGCATCGCCGAGGAAGACTTGAAACTGCGCGGTGAAGGCGAGCTGCTCGGCACGCGCCAGTCC
GGTACGCCGGGCTTCCGCATCGCCAGCCTCGAGGCGCATGCCGACCTGCTTGAGATCGCCCGCAAGGATGCCGCTTATCT
CATCGAGCGCGATCCTGAACTGACGAGTGAGAGAGGAACGGCGATCCGCACCCTGCTCTATCTCTTCCGTCGCGACGAGG
CGATCCGGTTCTTAAGAGCTGGATAA

Upstream 100 bases:

>100_bases
CCTGTCATGGCATTACCTTCCTGTTGATGCGCCTCTATAGACCCTGGAAAGCGGCTTGTCAGCCTTGCCAAGGCCGCATT
GTTCATCGCATTTTGGCCGC

Downstream 100 bases:

>100_bases
AGCGCGCCGCGGCGCGCTTTGGGCGACATGCATCAATCTGCCTTGGAAACCGCCACCGGCTTCGGCCGCGGCAGATGCTT
CTGCTTCGGTTCGGGCGGCT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MRPAILDPLFSPISGLPGVGPKIAELLVKLLGRETPEDCRVIDLIFHAPFSLIDRRNQPGIARAPQGAIVTITARVDRHQ
VPPRGKSNVPYRVFLHDETGELTLVFFRGQAAWLEKQLPVDAEVTVSGKVDWFNGRASMVHPDYIVRADEAENLPLVEPI
YPLTAGLSPKTLRKIIDAALPRFPELPEWIDQALAQKQGLPTIRDSFHMLHEPRDPADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVAGQPVQATGEVSAKILKSLPFSLTSSQNEAIAEVLKDMAGTERMLRLLQGDVGSGKTLVALMAMAAVIE
SGGQAVLMAPTEILARQHHATISKFAASAGLGIEILTGRTKGREREEILDRIASGAAQIIIGTHALFQDSVAYAKLMLAV
VDEQHRFGVHQRLRLTAKGISPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTITVPMERTGEIVGRLQSAL
AEGKKAYWICPLVEESEELDLMSAEERHATLVAALGPDIGLIHGRMSGPEKDAAMMAFKNGETRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKGPLGETGHARLSIMRETEDGFRIAEEDLKLRGEGELLGTRQS
GTPGFRIASLEAHADLLEIARKDAAYLIERDPELTSERGTAIRTLLYLFRRDEAIRFLRAG

Sequences:

>Translated_701_residues
MRPAILDPLFSPISGLPGVGPKIAELLVKLLGRETPEDCRVIDLIFHAPFSLIDRRNQPGIARAPQGAIVTITARVDRHQ
VPPRGKSNVPYRVFLHDETGELTLVFFRGQAAWLEKQLPVDAEVTVSGKVDWFNGRASMVHPDYIVRADEAENLPLVEPI
YPLTAGLSPKTLRKIIDAALPRFPELPEWIDQALAQKQGLPTIRDSFHMLHEPRDPADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVAGQPVQATGEVSAKILKSLPFSLTSSQNEAIAEVLKDMAGTERMLRLLQGDVGSGKTLVALMAMAAVIE
SGGQAVLMAPTEILARQHHATISKFAASAGLGIEILTGRTKGREREEILDRIASGAAQIIIGTHALFQDSVAYAKLMLAV
VDEQHRFGVHQRLRLTAKGISPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTITVPMERTGEIVGRLQSAL
AEGKKAYWICPLVEESEELDLMSAEERHATLVAALGPDIGLIHGRMSGPEKDAAMMAFKNGETRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKGPLGETGHARLSIMRETEDGFRIAEEDLKLRGEGELLGTRQS
GTPGFRIASLEAHADLLEIARKDAAYLIERDPELTSERGTAIRTLLYLFRRDEAIRFLRAG
>Mature_701_residues
MRPAILDPLFSPISGLPGVGPKIAELLVKLLGRETPEDCRVIDLIFHAPFSLIDRRNQPGIARAPQGAIVTITARVDRHQ
VPPRGKSNVPYRVFLHDETGELTLVFFRGQAAWLEKQLPVDAEVTVSGKVDWFNGRASMVHPDYIVRADEAENLPLVEPI
YPLTAGLSPKTLRKIIDAALPRFPELPEWIDQALAQKQGLPTIRDSFHMLHEPRDPADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVAGQPVQATGEVSAKILKSLPFSLTSSQNEAIAEVLKDMAGTERMLRLLQGDVGSGKTLVALMAMAAVIE
SGGQAVLMAPTEILARQHHATISKFAASAGLGIEILTGRTKGREREEILDRIASGAAQIIIGTHALFQDSVAYAKLMLAV
VDEQHRFGVHQRLRLTAKGISPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTITVPMERTGEIVGRLQSAL
AEGKKAYWICPLVEESEELDLMSAEERHATLVAALGPDIGLIHGRMSGPEKDAAMMAFKNGETRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKGPLGETGHARLSIMRETEDGFRIAEEDLKLRGEGELLGTRQS
GTPGFRIASLEAHADLLEIARKDAAYLIERDPELTSERGTAIRTLLYLFRRDEAIRFLRAG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=687, Percent_Identity=41.9213973799127, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787357, Length=404, Percent_Identity=38.6138613861386, Blast_Score=233, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76726; Mature: 76726

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAILDPLFSPISGLPGVGPKIAELLVKLLGRETPEDCRVIDLIFHAPFSLIDRRNQPG
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHEEHHHHHCCHHHHHCCCCCC
IARAPQGAIVTITARVDRHQVPPRGKSNVPYRVFLHDETGELTLVFFRGQAAWLEKQLPV
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHCCCC
DAEVTVSGKVDWFNGRASMVHPDYIVRADEAENLPLVEPIYPLTAGLSPKTLRKIIDAAL
CCEEEEECEEEEECCCCCEECCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHC
PRFPELPEWIDQALAQKQGLPTIRDSFHMLHEPRDPADIDAQAPARRRLAYDEFLAGQLS
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLVRQRLRKVAGQPVQATGEVSAKILKSLPFSLTSSQNEAIAEVLKDMAGTERMLRLLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GDVGSGKTLVALMAMAAVIESGGQAVLMAPTEILARQHHATISKFAASAGLGIEILTGRT
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KGREREEILDRIASGAAQIIIGTHALFQDSVAYAKLMLAVVDEQHRFGVHQRLRLTAKGI
CCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCC
SPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTITVPMERTGEIVGRLQSAL
CCEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
AEGKKAYWICPLVEESEELDLMSAEERHATLVAALGPDIGLIHGRMSGPEKDAAMMAFKN
HCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCCCCCCCCCEEEEEEC
GETRLLVATTVVEVGVDVPDATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKGP
CCEEEEEEEEEHHHCCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEEECC
LGETGHARLSIMRETEDGFRIAEEDLKLRGEGELLGTRQSGTPGFRIASLEAHADLLEIA
CCCCCCCEEEEEEECCCCCEEEHHHHEECCCCCEEEECCCCCCCEEEEEEHHHHHHHHHH
RKDAAYLIERDPELTSERGTAIRTLLYLFRRDEAIRFLRAG
HHCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPAILDPLFSPISGLPGVGPKIAELLVKLLGRETPEDCRVIDLIFHAPFSLIDRRNQPG
CCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHEEHHHHHCCHHHHHCCCCCC
IARAPQGAIVTITARVDRHQVPPRGKSNVPYRVFLHDETGELTLVFFRGQAAWLEKQLPV
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHCCCC
DAEVTVSGKVDWFNGRASMVHPDYIVRADEAENLPLVEPIYPLTAGLSPKTLRKIIDAAL
CCEEEEECEEEEECCCCCEECCCEEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHC
PRFPELPEWIDQALAQKQGLPTIRDSFHMLHEPRDPADIDAQAPARRRLAYDEFLAGQLS
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLVRQRLRKVAGQPVQATGEVSAKILKSLPFSLTSSQNEAIAEVLKDMAGTERMLRLLQ
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GDVGSGKTLVALMAMAAVIESGGQAVLMAPTEILARQHHATISKFAASAGLGIEILTGRT
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KGREREEILDRIASGAAQIIIGTHALFQDSVAYAKLMLAVVDEQHRFGVHQRLRLTAKGI
CCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCC
SPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTITVPMERTGEIVGRLQSAL
CCEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
AEGKKAYWICPLVEESEELDLMSAEERHATLVAALGPDIGLIHGRMSGPEKDAAMMAFKN
HCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEECCCCEEEEECCCCCCCCCCEEEEEEC
GETRLLVATTVVEVGVDVPDATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKGP
CCEEEEEEEEEHHHCCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEEECC
LGETGHARLSIMRETEDGFRIAEEDLKLRGEGELLGTRQSGTPGFRIASLEAHADLLEIA
CCCCCCCEEEEEEECCCCCEEEHHHHEECCCCCEEEECCCCCCCEEEEEEHHHHHHHHHH
RKDAAYLIERDPELTSERGTAIRTLLYLFRRDEAIRFLRAG
HHCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]