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The map label for this gene is gatB [H]

Identifier: 209549127

GI number: 209549127

Start: 1553731

End: 1555233

Strand: Direct

Name: gatB [H]

Synonym: Rleg2_1528

Alternate gene names: 209549127

Gene position: 1553731-1555233 (Clockwise)

Preceding gene: 209549124

Following gene: 209549128

Centisome position: 34.24

GC content: 62.08

Gene sequence:

>1503_bases
ATGACCCTTGTCGACGTCCGCACGCCTGATCCGAAACGCTTCATCCCCGGCGCCACCGGCGACTGGGAAGTCATCGTCGG
CATGGAAGTCCATGCCCAGGTGCTGTCCAATTCGAAGCTCTTCTCCGGCGCCTCGACGGAATTCGGCAAGCCACAGAATT
CCAACGTCTCGCTGGTCGATGCCGCCATGCCCGGCATGCTGCCTGTTATCAACGAGGAATGCGTCAGGCAGGCCGTGCGC
ACCGGTCTCGGCCTCAAGGCCCAGATCAACAAGCGCTCGCTCTTCGACCGCAAGAACTATTTCTATCCCGACCTGCCGCA
GGGCTATCAGATCTCGCAGTTCAAGGATCCGATCGTCGGCGAGGGCAAGATCGTCATTTCGCTCGGGCCGGACCGCCAAG
GCCAGTTCGAGGATATCGAGATCGGCATCGAGCGCCTGCACCTGGAGCAGGATGCCGGCAAGTCGCTGCATGACCAGCAT
GCGACCATGTCCTATGTCGATCTGAACCGCTCCGGCGTCGCGCTGATGGAGATCGTCTCCAAGCCCGACATGCGCTCGTC
CGACGAAGCCAAGGCCTATATGACCAAGCTGCGTTCGATCGTGCGTTATCTCGGCACCTGCGACGGCAACATGGACGAAG
GTTCGATGCGCGCCGACGTCAACGTCTCCGTCCGCCGCCCGGGCGAAGGTTTCGGCACGCGCTGCGAGATCAAGAACGTC
AACTCCATCCGCTTCATCGGCCAGGCGATCGAATATGAAGCCCGCCGCCAGATCGGCATTCTGGAAGATGGCGGCACGAT
CGAGCAGGAGACGCGGTTGTTCGACCCGAACAAGGGCGAGACACGTTCGATGCGCTCCAAGGAGGATGCGCACGACTATC
GTTATTTCCCCGATCCGGACCTGCTGCCGCTCGAGTTCGACGATGCCTTCGTCTCAGCACTCGCAGTCGATCTGCCGGAA
TTGCCTGACGACAAGAAGGAGCGCTTCGTGCGCGAGCTCGGCCTGTCGATCTACGACGCGTCGGTGCTCGTTTCCGAAAA
GGCGATCGCCGATTATTTCGAAGCCGTGGCCGCGGGCCGTGACGGCAAGACGGCGGCAAACTGGGTGATCAACGATCTGC
TCGGCGCGCTGAACCGCACCGGCAAGGATATCGAGCAGACGCCGGTTTCGCCGGCCCAGCTCGGCGCCATCATCGATCTG
ATCAAGGCTGGCACCATCTCCGGCAAGATCGCCAAGGATCTCTTCGAGATCGTGCTGACCGAGGGCGGTGATCCCGCCGA
GATCGTCGACGCGCGCGGCATGAAGCAGGTGACCGATACCGGCGCCATCGAGAAGGCCGTGGACGAGATCATCGCCGCCA
ATCCCGATCAGGTGGAAAAGGTCAAGGCGAAGCCGACCCTGGCCGCATGGTTCGTCGGCCAGGTGATGAAGGCGACCGGC
GGCAAGGCCAATCCGCAGGCCGTCCAGGCGCTCGTCAAGGCAAAGCTCGGCATCGAGGAATAA

Upstream 100 bases:

>100_bases
GGGCAACGGCCACTGCTACACAATTCCGCGAAAGCCTTGCACCGACATCCCATTTCCTTTACCTCACCAATGGAAAAGAA
CAGCCTGCAAAGAGTATCAG

Downstream 100 bases:

>100_bases
GCGGTGTATTTCGTCCGCACCGCCGGCGAGCGCGACCTGGAGAAGGTCCGCGCCCTGCTGGTGGAAAGTTTTCACGCGAC
CTATGACGGCCTCCATGGCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTLVDVRTPDPKRFIPGATGDWEVIVGMEVHAQVLSNSKLFSGASTEFGKPQNSNVSLVDAAMPGMLPVINEECVRQAVR
TGLGLKAQINKRSLFDRKNYFYPDLPQGYQISQFKDPIVGEGKIVISLGPDRQGQFEDIEIGIERLHLEQDAGKSLHDQH
ATMSYVDLNRSGVALMEIVSKPDMRSSDEAKAYMTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
NSIRFIGQAIEYEARRQIGILEDGGTIEQETRLFDPNKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVSALAVDLPE
LPDDKKERFVRELGLSIYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNRTGKDIEQTPVSPAQLGAIIDL
IKAGTISGKIAKDLFEIVLTEGGDPAEIVDARGMKQVTDTGAIEKAVDEIIAANPDQVEKVKAKPTLAAWFVGQVMKATG
GKANPQAVQALVKAKLGIEE

Sequences:

>Translated_500_residues
MTLVDVRTPDPKRFIPGATGDWEVIVGMEVHAQVLSNSKLFSGASTEFGKPQNSNVSLVDAAMPGMLPVINEECVRQAVR
TGLGLKAQINKRSLFDRKNYFYPDLPQGYQISQFKDPIVGEGKIVISLGPDRQGQFEDIEIGIERLHLEQDAGKSLHDQH
ATMSYVDLNRSGVALMEIVSKPDMRSSDEAKAYMTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
NSIRFIGQAIEYEARRQIGILEDGGTIEQETRLFDPNKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVSALAVDLPE
LPDDKKERFVRELGLSIYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNRTGKDIEQTPVSPAQLGAIIDL
IKAGTISGKIAKDLFEIVLTEGGDPAEIVDARGMKQVTDTGAIEKAVDEIIAANPDQVEKVKAKPTLAAWFVGQVMKATG
GKANPQAVQALVKAKLGIEE
>Mature_499_residues
TLVDVRTPDPKRFIPGATGDWEVIVGMEVHAQVLSNSKLFSGASTEFGKPQNSNVSLVDAAMPGMLPVINEECVRQAVRT
GLGLKAQINKRSLFDRKNYFYPDLPQGYQISQFKDPIVGEGKIVISLGPDRQGQFEDIEIGIERLHLEQDAGKSLHDQHA
TMSYVDLNRSGVALMEIVSKPDMRSSDEAKAYMTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNVN
SIRFIGQAIEYEARRQIGILEDGGTIEQETRLFDPNKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVSALAVDLPEL
PDDKKERFVRELGLSIYDASVLVSEKAIADYFEAVAAGRDGKTAANWVINDLLGALNRTGKDIEQTPVSPAQLGAIIDLI
KAGTISGKIAKDLFEIVLTEGGDPAEIVDARGMKQVTDTGAIEKAVDEIIAANPDQVEKVKAKPTLAAWFVGQVMKATGG
KANPQAVQALVKAKLGIEE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=41.2121212121212, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=40.6040268456376, Blast_Score=212, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=533, Percent_Identity=31.1444652908068, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=37.7319587628866, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54716; Mature: 54585

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVDVRTPDPKRFIPGATGDWEVIVGMEVHAQVLSNSKLFSGASTEFGKPQNSNVSLVD
CEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVRQAVRTGLGLKAQINKRSLFDRKNYFYPDLPQGYQISQFKDPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCCCCCCCCHHCCCCCCC
EGKIVISLGPDRQGQFEDIEIGIERLHLEQDAGKSLHDQHATMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCHHHHHHHC
KPDMRSSDEAKAYMTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFIGQAIEYEARRQIGILEDGGTIEQETRLFDPNKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHHCEEEEECCCCCCHHHCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVSALAVDLPELPDDKKERFVRELGLSIYDASVLVSEKAIADYFEAVAAGR
CEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCC
DGKTAANWVINDLLGALNRTGKDIEQTPVSPAQLGAIIDLIKAGTISGKIAKDLFEIVLT
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EGGDPAEIVDARGMKQVTDTGAIEKAVDEIIAANPDQVEKVKAKPTLAAWFVGQVMKATG
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCC
GKANPQAVQALVKAKLGIEE
CCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLVDVRTPDPKRFIPGATGDWEVIVGMEVHAQVLSNSKLFSGASTEFGKPQNSNVSLVD
EEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
AAMPGMLPVINEECVRQAVRTGLGLKAQINKRSLFDRKNYFYPDLPQGYQISQFKDPIVG
CCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCCCCCCCCCCCHHCCCCCCC
EGKIVISLGPDRQGQFEDIEIGIERLHLEQDAGKSLHDQHATMSYVDLNRSGVALMEIVS
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCHHHHHHHC
KPDMRSSDEAKAYMTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFIGQAIEYEARRQIGILEDGGTIEQETRLFDPNKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHHCEEEEECCCCCCHHHCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVSALAVDLPELPDDKKERFVRELGLSIYDASVLVSEKAIADYFEAVAAGR
CEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCC
DGKTAANWVINDLLGALNRTGKDIEQTPVSPAQLGAIIDLIKAGTISGKIAKDLFEIVLT
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EGGDPAEIVDARGMKQVTDTGAIEKAVDEIIAANPDQVEKVKAKPTLAAWFVGQVMKATG
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCC
GKANPQAVQALVKAKLGIEE
CCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA