Click here to switch to the map view.

The map label for this gene is dusC [H]

Identifier: 209400918

GI number: 209400918

Start: 2999730

End: 3000680

Strand: Reverse

Name: dusC [H]

Synonym: ECH74115_3266

Alternate gene names: 209400918

Gene position: 3000680-2999730 (Counterclockwise)

Preceding gene: 209397299

Following gene: 209398358

Centisome position: 53.85

GC content: 53.94

Gene sequence:

>951_bases
ATGCGTGTGTTACTGGCTCCGATGGAGGGAGTGCTTGACTCTCTGGTGCGTGAATTGCTGACCGAAGTTAACGACTACGA
TCTGTGCATCACCGAGTTCGTCCGCGTGGTGGATCAACTGCTGCCGGTGAAAGTCTTTCATCGCATTTGCCCTGAGCTAC
AAAACGCCAGCCGGACACCATCCGGTACGCTGGTGCGCGTGCAGTTGTTGGGTCAGTTCCCACAATGGCTGGCAGAGAAC
GCCGCCCGTGCGGTGGAATTAGGTTCCTGGGGCGTGGATCTCAATTGCGGCTGCCCGTCGAAAACGGTTAACGGTAGCGG
TGGTGGGGCGACGTTACTCAAAGATCCTGAACTCATCTACCAGGGTGCAAAAGCGATGCGTGAAGCTGTACCGGCGCATT
TGCCCGTCAGCGTGAAAGTGCGTCTGGGCTGGGACAGCGGTGAGAAGAAATTTGAAATCGCCGATGCGGTTCAACAGGCT
GGTGCTACGGAGCTGGTGGTGCATGGACGGACAAAAGAGCAGGGTTACCGCGCGGAGCATATTGACTGGCAGGCGATTGG
CGAGATTCGCCAGCGGCTGAATATTCCGGTGATTGCCAACGGCGAAATCTGGGACTGGCAGAGCGCGCAAGAATGCATGG
CGATCAGCGGCTGCGACTCAGTGATGATTGGTCGCGGGGCGCTCAATATTCCCAACCTGAGCCGGGTAGTAAAATATAAC
GAACCGCGAATGCCGTGGCCGGAGGTGGTTGCTTTGCTGCAAAAATATACCCGTCTGGAAAAGCAGGGCGATACCGGGTT
ATATCACGTAGCGCGGATTAAACAGTGGTTGAGTTATTTGCGTAAAGAATACGATGAAGCAACAGAATTATTTCAGCATG
TTCGTGTGTTGAATAATTCCCCTGATATTGCAAGGGCTATTCAGGCAATTGATATCGGTAAGCTGCGATAA

Upstream 100 bases:

>100_bases
AGGGTGAGGGTAAAAATCTTGCCCGAATTAATCAGTAAGTCAGTTTGTCTGCAAACACAGGAGGCGCTAACATAGCGCCT
CATTTTTTGCGGGTGATGAT

Downstream 100 bases:

>100_bases
TAGTGGAATCGCAGTTTATCAGAAACAATGAATCGCTTATTCAGCTTCATCATCTTGTTGCGCTCCTTTCATGAGCTAAG
CCACATATTGCCACTGGCGC

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGCDSVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR

Sequences:

>Translated_316_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGCDSVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR
>Mature_316_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGCDSVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIGKLR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=31.1688311688312, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=28.996282527881, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI239788483, Length=225, Percent_Identity=32, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI239788462, Length=225, Percent_Identity=32, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=98.7301587301587, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=28.3950617283951, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=25.8964143426295, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=39.2857142857143, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=34.4155844155844, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=27.0642201834862, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=30.8457711442786, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585320, Length=217, Percent_Identity=31.7972350230415, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGCDSVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIGKLR
CHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQECMAISGCDSVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIGKLR
CHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]