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The map label for this gene is intS [H]

Identifier: 209400784

GI number: 209400784

Start: 4684091

End: 4685344

Strand: Direct

Name: intS [H]

Synonym: ECH74115_5032

Alternate gene names: 209400784

Gene position: 4684091-4685344 (Clockwise)

Preceding gene: 209396213

Following gene: 209397454

Centisome position: 84.06

GC content: 47.85

Gene sequence:

>1254_bases
TTGGGGGTATCAGGTTGGTTACTGGCGGAAATTCCCCCAGGAGGATGGATTCATCAAAAGCTGGAGGATGTCATGGCGCT
AACCGATGTTAAAGTGAAAACCGCGAAGCCAAAAGAAAGGCCCTATAAGCTGGCTGATGGCGGAGGTATGTATCTGCTGA
TTAACGCTAATGGTTCCAAATACTGGCGTATGAAGTACCGCTTCGCCGGTAAAGAAAAGATGCTGTCTATTGGCGTATAC
CCCGATGTAACGCTTGCCGACGCACGTGAGAAACGCAGTGAAGCCAGGAAAATACTTGCGGCTGGTGGCGATCCGGGTGA
GGCAAAAAAAGAAGAAAAAATAGCGCTGCAAATGAGTTTGAAAAACACTTTCGAGGCCGTTGCTCGCGAGTGGCATCAGA
CGAAAGCCGATCGCTGGTCACTGCGCTACCGCGATGAAATAATCGACACCTTTGAGAAAGATATCTTCCCCTACATCGGC
AAGCGACCTATTGCCGAAATCAAGCCGATGGAGCTATTGGAAGCACTACGTAAGATGGAGAAACGTGGAGCACTGGAGAA
AATGCGCAAGGTCCGCCAACGTTGTGGTGAGGTGTTTCGCTATGCAATTGTTACTGGTCGAGCCGACTATAACCCTGCTC
CCGATCTTGCCAGTGCTTTAGCTACACCGAAAAAAGTACATTTTCCCTTTCTTACTGCCAATGAACTTCCCCACTTCCTT
ACTGATCTGGCGGGTTATACCGGAAGTATCATCACTAAGACAGCTACTCAGATCATTATGTTAACCGGTGTACGAACGCA
AGAGTTGCGTTTCGCGCATTGGGAGGATATTGATTTTGAGGCAAAATTATGGGAGATCCCGGCAGAAGTAATGAAGATGA
AACGGCCTCATATTGTGCCGCCCTCTGAGCAGGTTATTGCGCTATTTAAGCAACTTGAACCAATCTCAAAACATCATCCT
CTGGTCTTTATCGGTAGGAACGATCCTCGCAAGCCAATTAGTAAGGAGAGCATTAACCAGGTCATTGAATTGCTGGGGTA
TAAGGGAAGACTCACAGGGCACGGTTTCAGGCATACCATGAGCACAATTCTGCACGAACAAGGCTTTAATTCTGCCTGGA
TTGAAATGCAGTTGGCTCATGTGGATAAAAACTCCATCAGGGGTACCTATAACCACGCCCAGTATCTCGATGGTCGCCGT
GAAATGATGCAATGGTACGCTGACTACATTGATTCACTTTCTGAGCTGGCCTGA

Upstream 100 bases:

>100_bases
GACATCAACAAAGCCCCTTATAATCCCTTATATATCAGGTTTTCTTTGTATCCTGAGGTCAAACGACTTTAACCCATATC
AAGTTGTTTGTGGGGGTGCA

Downstream 100 bases:

>100_bases
TGAATGAAAGTTCGGTGACACAAAAATAATGAATAACCATCCAGCCCCAACAGGCGGATGGTTGTAGTGGCACAGTAAAT
TTGGCCACCTGATTAAAGGT

Product: integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGVSGWLLAEIPPGGWIHQKLEDVMALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRFAGKEKMLSIGVY
PDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEAVAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIG
KRPIAEIKPMELLEALRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFL
TDLAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVPPSEQVIALFKQLEPISKHHP
LVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRR
EMMQWYADYIDSLSELA

Sequences:

>Translated_417_residues
MGVSGWLLAEIPPGGWIHQKLEDVMALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRFAGKEKMLSIGVY
PDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEAVAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIG
KRPIAEIKPMELLEALRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFL
TDLAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVPPSEQVIALFKQLEPISKHHP
LVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRR
EMMQWYADYIDSLSELA
>Mature_416_residues
GVSGWLLAEIPPGGWIHQKLEDVMALTDVKVKTAKPKERPYKLADGGGMYLLINANGSKYWRMKYRFAGKEKMLSIGVYP
DVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSLKNTFEAVAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGK
RPIAEIKPMELLEALRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLT
DLAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVPPSEQVIALFKQLEPISKHHPL
VFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRRE
MMQWYADYIDSLSELA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=390, Percent_Identity=48.4615384615385, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=41.7948717948718, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1788974, Length=386, Percent_Identity=39.6373056994819, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47777; Mature: 47646

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVSGWLLAEIPPGGWIHQKLEDVMALTDVKVKTAKPKERPYKLADGGGMYLLINANGSK
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCEEEEEECCCCE
YWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSL
EEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KNTFEAVAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFL
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHH
TDLAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
HHHHCCCCHHHHHHHHHEEEEECCCHHHHCEECCCCCCCCHHHHHCCHHHHHCCCCCCCC
PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTM
CHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
STILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSELA
HHHHHHCCCCCHHEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GVSGWLLAEIPPGGWIHQKLEDVMALTDVKVKTAKPKERPYKLADGGGMYLLINANGSK
CCCCCEEEECCCCCHHHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCEEEEEECCCCE
YWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGEAKKEEKIALQMSL
EEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
KNTFEAVAREWHQTKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFL
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCEEEHHHHHHHH
TDLAGYTGSIITKTATQIIMLTGVRTQELRFAHWEDIDFEAKLWEIPAEVMKMKRPHIVP
HHHHCCCCHHHHHHHHHEEEEECCCHHHHCEECCCCCCCCHHHHHCCHHHHHCCCCCCCC
PSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTM
CHHHHHHHHHHHCCHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
STILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSELA
HHHHHHCCCCCHHEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]