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The map label for this gene is wcaF [H]

Identifier: 209400766

GI number: 209400766

Start: 2768550

End: 2769098

Strand: Reverse

Name: wcaF [H]

Synonym: ECH74115_2989

Alternate gene names: 209400766

Gene position: 2769098-2768550 (Counterclockwise)

Preceding gene: 209396416

Following gene: 209400542

Centisome position: 49.7

GC content: 48.27

Gene sequence:

>549_bases
ATGCAGGATTTAAGCGGTTTCTCGGTGCCGAAAGGGTTCCGGGGCGGCAACGCTATTAAAGTGCAATTGTGGTGGGCAGT
ACAGGCAACAATATTTGCCTGGTCGCCACAAGTATTGTATCGCTGGCGGGCATTTTTATTACGTTTATTCGGCGCAAAAA
TAGGAAAAAACGTAGTTATTCGTCCGTCAGTAAAAATTACCTATCCGTGGAAATTAACCTTAGGTGATTACGTGTGGGTC
GGCGATGACGTCAATTTATATACCCTCGGTGAAATAACCATTGGCGCACATTCGGTGATATCGCAAAAAAGTTATTTATG
CACCGGGAGCCACGACCATGCAAGTCAACATTTCACCATTAACGCCACGCCTATTGTGATTGGCGAGAAATGCTGGCTGG
CAACCGATGTTTTTGTTGCCCCAGGCGTCACGATTGGCGACGGCACCGTCGTGGGTGCGCGAAGCAGTGTTTTTAAATCG
CTTCCGGCAAATGTGGTTTGCCGGGGGAATCCCGCAGTGGTGATACGCAAACGCGTTGAAACTGAATAA

Upstream 100 bases:

>100_bases
TGTGCCTGGCTTTTGGGTTGAATTATCCTGGCATTTACGCCAGCGTACTACCTCAAAGACGAAAGCCTTATATAACAAAA
GCTGAATATAAGGGAAAACG

Downstream 100 bases:

>100_bases
ATTCAAAAAAAATACAGAGGAATAAGACATGTCAAAAGTCGCTCTCATCACCGGTGTAACCGGACAAGACGGTTCTTACC
TGGCAGAGTTTCTGCTGGAA

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRKRVETE

Sequences:

>Translated_182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRKRVETE
>Mature_182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYVWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRKRVETE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=182, Percent_Identity=98.9010989010989, Blast_Score=369, Evalue=1e-104,
Organism=Escherichia coli, GI1786537, Length=138, Percent_Identity=32.6086956521739, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788345, Length=118, Percent_Identity=35.5932203389831, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786664, Length=121, Percent_Identity=30.5785123966942, Blast_Score=64, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=153, Percent_Identity=28.1045751633987, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 19989; Mature: 19989

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00095 C5_MTASE_2 ; PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTI
CCCEEEEEEEEEEECCEEEECCCCCEEEEEEEEECCHHHHCCCCEEEECCCCCCCCEEEE
NATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRKRVE
ECCEEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCEEEEEEECC
TE
CC
>Mature Secondary Structure
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTLGDYVWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTI
CCCEEEEEEEEEEECCEEEECCCCCEEEEEEEEECCHHHHCCCCEEEECCCCCCCCEEEE
NATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRKRVE
ECCEEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCEEEEEEECC
TE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]