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The map label for this gene is rlmN [H]

Identifier: 209400754

GI number: 209400754

Start: 3463387

End: 3464541

Strand: Reverse

Name: rlmN [H]

Synonym: ECH74115_3742

Alternate gene names: 209400754

Gene position: 3464541-3463387 (Counterclockwise)

Preceding gene: 209399802

Following gene: 209396298

Centisome position: 62.18

GC content: 52.64

Gene sequence:

>1155_bases
ATGTCTGAACAATTAGTCACACCTGAAAACGTCACCACCAAAGATGGAAAAATCAACCTGCTGGATCTCAATCGTCAGCA
GATGCGGGAGTTTTTTAAAGATTTAGGTGAAAAACCCTTCCGCGCCGATCAGGTGATGAAGTGGATGTATCACTATTGCT
GCGACAATTTTGATGAGATGACCGACATCAACAAAGTGTTGCGCGGCAAACTGAAAGAGGTGGCGGAGATCCGCGCACCG
GAAGTGGTTGAAGAACAGCGTTCATCTGACGGCACCATTAAATGGGCGATTGCCGTGGGTGACCAGCGTGTCGAAACGGT
GTACATCCCGGAAGACGACCGCGCTACGCTCTGCGTCTCTTCGCAGGTGGGGTGTGCGCTGGAGTGTAAATTCTGTTCCA
CCGCCCAGCAGGGCTTTAACCGCAACCTGCGGGTATCGGAAATTATTGGCCAGGTGTGGCGTGCGGCGAAAATCGTCGGC
GCAGCAAAAGTCACCGGTCAGCGCCCAATCACTAACGTAGTGATGATGGGCATGGGCGAGCCGCTGCTCAACCTGAACAA
CGTCGTTCCGGCGATGGAAATCATGCTTGATGATTTCGGTTTTGGTCTGTCTAAACGTCGCGTGACGCTTTCCACTTCCG
GTGTAGTTCCAGCGCTGGATAAACTGGGCGATATGATCGATGTTGCGCTGGCAATTTCCCTGCACGCGCCGAACGACGAA
ATTCGTGACGAAATTGTGCCGATCAACAAAAAGTACAATATCGAAACGTTCCTTGCTGCGGTGCGCCGTTATCTGGAGAA
ATCCAACGCCAATCAGGGCCGAGTCACTATTGAATACGTGATGCTTGACCACGTTAACGACGGCACTGAACACGCGCACC
AACTGGCGGAACTGCTGAAAGATACGCCGTGTAAGATCAACCTGATCCCGTGGAACCCGTTCCCGGCTGCGCCGTATGGA
CGCAGCTCGAACAGCCGTATCGACCGTTTCTCAAAAGTGCTGATGAGCTACGGTTTCACCACTATTGTGCGTAAAACGCG
TGGTGATGATATCGATGCTGCCTGTGGTCAGTTGGCGGGCGATGTTATCGACCGTACGAAACGTACCCTGCGTAAACGGA
TGCAGGGTGAAGCCATCGACATTAAAGCGGTCTGA

Upstream 100 bases:

>100_bases
GTACAATGCAGCGCCCCCGGACGAGCAGCCGCTCACCGGGGCGTTTCTTTTTTCAACCCTCCAGGGGCCATAACGTGTAA
TAACGAGGCCGGAATAGCAT

Downstream 100 bases:

>100_bases
TAGCCATTACGCCACGGTTACTTTATGTACCGTGGCGTAATGTTTTATGAATGAAGTATTAAGTTGCAATTGTCTGGTCA
ATATTAACGGTGCGTTTTTG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV

Sequences:

>Translated_384_residues
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAP
EVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYG
RSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV
>Mature_383_residues
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPE
VVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGA
AKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDEI
RDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPAAPYGR
SSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=384, Percent_Identity=99.7395833333333, Blast_Score=797, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 43100; Mature: 42969

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDIKAV
HHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQVMKWMYHYCCDNFDEM
CCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHH
TDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQRVETVYIPEDDRATLCVS
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEE
SQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVGAAKVTGQRPITNVVMMGMGE
CCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
PLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDMIDVALAISLHAPNDE
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHEEHHEEEEECCCHH
IRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVTIEYVMLDHVNDGTEHAHQLAELLK
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHC
DTPCKINLIPWNPFPAAPYGRSSNSRIDRFSKVLMSYGFTTIVRKTRGDDIDAACGQLAG
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHH
DVIDRTKRTLRKRMQGEAIDIKAV
HHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA