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The map label for this gene is intB [H]

Identifier: 209400524

GI number: 209400524

Start: 5431168

End: 5431995

Strand: Direct

Name: intB [H]

Synonym: ECH74115_5797

Alternate gene names: 209400524

Gene position: 5431168-5431995 (Clockwise)

Preceding gene: 209399960

Following gene: 209397351

Centisome position: 97.47

GC content: 49.15

Gene sequence:

>828_bases
ATGGCCCTAACGGATACTAAAGTTCGCTCTGCGAAACCTGAGGAGAAAGAGTATTCACTTGTTGACGGCGACGGCATGTC
CTTACTTGTAAAACCTGGCGGTTCAAAGTATTGGCGATTTCGTTTCCGTTTTGGCGGTAAACAACATTTGATGGCGTTCG
GCGTTTACCCGGATGTTTCACTGGCGGATGCGCGGAAGAAAAGGGAAGAGGCCAGAAAGCTGGTAGCTGCTGGTATCGAT
CCTCGTGAGCATAAGCGTGCTGTGAAAGAAGAGCAGGCGAAAGAGATTATTACTTTCGAGAAGGTTGCCAGAGAGTGGCT
CGTAACCAACCAAAAATGGTCGGAAGATCATGCTAATCGTGTAAAAAAGAGCCTGGAGGACAATATCTTCCCGACAATTG
GTACTCGCAACATTGCTGAACTAGGCACCCGTGATCTGTTAATTCCTATTAAAGCCGTGGAGAAATCCGGGCGTCTTGAA
GTGGCTTCCCGCCTTCAACAGCGCACCACGGCCATCATGCGTTATGCAGTGCAAAGCGGGTTAATTGATTACGATCCGGC
GCAAGAGATGTCGGGGGCGGTTGCTTCCAGTAATCGACAGCATCGTCCGGCGCTGGAGTTAAAGCGTATCCCTGAGTTGC
TTGACAAAATAGACAGCTACACCGGCAGACCGCTAACCCACTGTACGACAGAACTCACTCTGCTTATCTTTATTCGGTCC
AGTAAGCTGCATTTTGCTCGCTGGTCAGAAATCGATTTTGAAACGTCAATGTGGACCATCCCTCTTGATTGGTATTGCAG
TAAGCAGAGGGTTGGACTGGTACAGTAA

Upstream 100 bases:

>100_bases
ACCAACCCACATCAACAGGCTTGTGAGTATACTAATGAGTATATTTGCCGATTCGATATTGCTTGTATACTCACGAGATA
CCAATGGAAGGAAGACCATT

Downstream 100 bases:

>100_bases
ATATCCCGCATAATCGTGCCATTCACATTTAGAGATCCTCCGGCATAATCAATCTGCCAATGAAGGAGATCGCTATTCGT
AAAGCCCCTTTTACTGAGCA

Product: prophage P4 integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGID
PREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEDHANRVKKSLEDNIFPTIGTRNIAELGTRDLLIPIKAVEKSGRLE
VASRLQQRTTAIMRYAVQSGLIDYDPAQEMSGAVASSNRQHRPALELKRIPELLDKIDSYTGRPLTHCTTELTLLIFIRS
SKLHFARWSEIDFETSMWTIPLDWYCSKQRVGLVQ

Sequences:

>Translated_275_residues
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGID
PREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEDHANRVKKSLEDNIFPTIGTRNIAELGTRDLLIPIKAVEKSGRLE
VASRLQQRTTAIMRYAVQSGLIDYDPAQEMSGAVASSNRQHRPALELKRIPELLDKIDSYTGRPLTHCTTELTLLIFIRS
SKLHFARWSEIDFETSMWTIPLDWYCSKQRVGLVQ
>Mature_274_residues
ALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDP
REHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEDHANRVKKSLEDNIFPTIGTRNIAELGTRDLLIPIKAVEKSGRLEV
ASRLQQRTTAIMRYAVQSGLIDYDPAQEMSGAVASSNRQHRPALELKRIPELLDKIDSYTGRPLTHCTTELTLLIFIRSS
KLHFARWSEIDFETSMWTIPLDWYCSKQRVGLVQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=263, Percent_Identity=39.9239543726236, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI145693166, Length=263, Percent_Identity=32.6996197718631, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1788690, Length=259, Percent_Identity=30.8880308880309, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 31505; Mature: 31373

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVS
CCCCCCCHHCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCH
LADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEDHANR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VKKSLEDNIFPTIGTRNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSG
HHHHHHCCCCCCCCCCHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYDPAQEMSGAVASSNRQHRPALELKRIPELLDKIDSYTGRPLTHCTTELTLLIFIRS
CCCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
SKLHFARWSEIDFETSMWTIPLDWYCSKQRVGLVQ
CCEEEEECCCCCCCCCEEEEEHHHEECCCCCCCCC
>Mature Secondary Structure 
ALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVS
CCCCCCHHCCCCCCCCEEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCH
LADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEDHANR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VKKSLEDNIFPTIGTRNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSG
HHHHHHCCCCCCCCCCHHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYDPAQEMSGAVASSNRQHRPALELKRIPELLDKIDSYTGRPLTHCTTELTLLIFIRS
CCCCCCHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
SKLHFARWSEIDFETSMWTIPLDWYCSKQRVGLVQ
CCEEEEECCCCCCCCCEEEEEHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]