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The map label for this gene is mamA

Identifier: 209400500

GI number: 209400500

Start: 855984

End: 856433

Strand: Reverse

Name: mamA

Synonym: ECH74115_0832

Alternate gene names: 209400500

Gene position: 856433-855984 (Counterclockwise)

Preceding gene: 209398431

Following gene: 209399739

Centisome position: 15.37

GC content: 49.78

Gene sequence:

>450_bases
ATGAAGAAAGCAACACTTGTGATTGGCGTTATTGGCGCGGACTGCCATGCAGTAGGCAATAAAGTTCTGGACCGCGTTTT
TAGCAACCACGACTTCAGAGTCATTAATCTGGGCGTAATGGTCAGCCAGGACGAATATATTGATGCTGCCATTGAAACTG
GCGCTGACGCGATTGTCGTCTCCTCTATCTATGGGCATGGCGATATCGACTGTCTGGGCATGCGCGAACGCTGCATCGAG
CGTGGACTGGGGGACATCCTGCTCTATGTGGGCGGTAACCTGGTGGTCGGAAAACATGACTTCGCTGACGTGGAAACCAA
ATTCAAAGAGATGGGATTTGATCGCGTCTTTGCCCCAAGTCATGACTTAGAAGATGTTTGCCAGCTGATGGCCCACGATA
TCAACCAACGTCATGACGTTGATACTCGTATTCTTGAAGAGGCTATCTGA

Upstream 100 bases:

>100_bases
GATAAAGACACTCCTGTGATATTAAACCTGTGCCGTTATGAACGGCCATAAATACGACGTGTGTAACTTATTTTACTCTC
TGTCTTACAAAGGATTACCC

Downstream 100 bases:

>100_bases
TGCAAATCGTCTCTGTCGATATTGGATCGACGTGGACCAAAGCAGCCCTCTTTACCCGGGAGGGGGACGCGTTAACGTTG
GTTAACCACGTACTGACCCC

Product: methylaspartate mutase subunit S

Products: NA

Alternate protein names: Glutamate mutase subunit sigma

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQDEYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIE
RGLGDILLYVGGNLVVGKHDFADVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI

Sequences:

>Translated_149_residues
MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQDEYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIE
RGLGDILLYVGGNLVVGKHDFADVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI
>Mature_149_residues
MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQDEYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIE
RGLGDILLYVGGNLVVGKHDFADVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI

Specific function: Unknown

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAMA_ECO57 (P58620)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85575
- PIR:   D90724
- RefSeq:   NP_286456.1
- RefSeq:   NP_308791.1
- ProteinModelPortal:   P58620
- SMR:   P58620
- EnsemblBacteria:   EBESCT00000024294
- EnsemblBacteria:   EBESCT00000058419
- GeneID:   917503
- GeneID:   957856
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0895
- KEGG:   ecs:ECs0764
- GeneTree:   EBGT00050000012934
- HOGENOM:   HBG672318
- OMA:   VIGSDCH
- ProtClustDB:   PRK02261
- BioCyc:   ECOL83334:ECS0764-MONOMER
- HAMAP:   MF_00526
- InterPro:   IPR006158
- InterPro:   IPR006394
- Gene3D:   G3DSA:3.40.50.280
- TIGRFAMs:   TIGR01501

Pfam domain/function: PF02310 B12-binding; SSF52242 Cbl-bd

EC number: =5.4.99.1

Molecular weight: Translated: 16430; Mature: 16430

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS51332 B12_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQDEYIDAAIETGADAIVV
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHCCCCEEEE
SSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFADVETKFKEMGFDRVFAPS
EECCCCCCEEEECHHHHHHHCCHHHEEEEECCEEEECCCCHHHHHHHHHHCCCCEEECCC
HDLEDVCQLMAHDINQRHDVDTRILEEAI
CCHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQDEYIDAAIETGADAIVV
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHCCCCEEEE
SSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFADVETKFKEMGFDRVFAPS
EECCCCCCEEEECHHHHHHHCCHHHEEEEECCEEEECCCCHHHHHHHHHHCCCCEEECCC
HDLEDVCQLMAHDINQRHDVDTRILEEAI
CCHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796