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The map label for this gene is rep [C]

Identifier: 209400456

GI number: 209400456

Start: 5461562

End: 5463676

Strand: Direct

Name: rep [C]

Synonym: ECH74115_5811

Alternate gene names: 209400456

Gene position: 5461562-5463676 (Clockwise)

Preceding gene: 209399692

Following gene: 209399448

Centisome position: 98.02

GC content: 50.02

Gene sequence:

>2115_bases
ATGACGGTGCAAACGCCCAAGGTGGCACTTTCTGATGGTTTTCTGGGCGCATTCGCGCGGATCCCAAAGGCACAGCAAAA
AAAGGTGCAGGAATTCATCTCCAAATTCCGTCAGGACCCGACCAGTAATGGACTTAACTATGAAAAGATCCACGATGCGC
GCAGTAAAAATGTCCATTCGGTGCGAATCGATCAAACTTATCGCGGCATTGTTCTGAAACCTGAACAGGGTGCCCTGTAT
ATGCTGATGTGGGTGGATAAACACGACGAAGCATATGACTGGGCGCGGCGGCATGATTGTTCAATTCACCCGGTTACCGG
CGCGATTCAGGTCATTGATATTAGCTATATCAAACCGGCAGCGGAAACAGTGGTTGATAAACCGAAACTCTTCGCTGCTT
ACAGCGCGGAACAGATTCTGGCTCTGGGTGTGCCGCCTGTCTTTATTGATCAGGTGATGGCGCTTACCGATGAAGCCGGG
CTTAATCAACTTGAAAGCATAATGCCAGCAGAAGCCTGGGAACCTCTGCACTGGCTGGCAGAAGGGCTGGATTACCAGGA
AGTGCTGGAAGAGTTTAACGATCATCGTGATGAGCCTGTTGATACCAACGACTTTATGGAGGCGATTGAACGAAGTAAAC
GTCGCTTCCATGTCGTTGAAAATGAACAGGAGCTGTTGCAGATGCTTAATGCGCCTTTAGAAAAATGGCGTGTATTTCTG
CATCCGAGCCAGCGAAAATTGGTGGAGTCGCCCGCTAATGGTCCGGTACGAGTACTGGGCGGTGCAGGTACAGGGAAAAC
GGTTGTTGCGATGCACCGTGCGCGGTGGTTATCTCAGCGTCTGGCAGATAAACCCGGTAAGAAAGTACTTTTCACGACCT
TTACGCGTAACCTGGCGGCAGATATTCGCGCTAATCTCCAGCGGTTGTGTACGCGAGAAGAGATGGCGCGTATTGACGTC
GTTAACATTGATGCCTGGATGAGCGACCAACTAAAACGCCACGGTTATGATTTCCGCGTGGTATACGACAGCGATGAAGG
GCGACGCAAATGCTGGAACTATGCCTTGCAGCAGGCACCTGCCGAACTGGGGCTACCTGATAATTTCTATGCTGAAGAGT
GGCAACGCGTTGTCCAGCCAAATGCGGTTTACACCCGGGAGGAATACCTCAAAGTCAGCCGGGTTGGACGTGGTACAGCG
TTATCCCGTATTCAACGGGCGAAAATCTGGCCAGTCTTTGAAGAATTCAGAGCACAAATGGCGCGTGCCAAACTGCGCGA
AATGGGCGATGCCATGCATGAGGCTATTGTTCTGTTTAAAGAAAAGCAGGTCCAACTACCTTATAGCAGTATTGTGGTCG
ATGAAGCTCAAGATATCGGTGCCCCGGCATTTACTCTTATTCGCAGCCTGGTTCCCGAGTCGCCCAACGATCTGTTTATT
GTCGGGGATGGGCATCAGCGTATTTATCGCAATAAGGTCGTTCTTGGTCAGTGTGGTATTAACGTCCGTGGTCGTCGCAG
TAAAAAATTGAAGATTAACTATCGGACCACAGAAGAGACGCGTCAATTTGCTGTTGGGCTGTTAACTGGTGTGAAGGTTG
ATAACCTCGATGGCGAAGCGGATACCAGTAATGATTATTTGTCATTACTGCACGGGGAAAAGCCCATGATTACGCATGCG
GCAGATTTCAAAGAAGAGGCTGCAACGCTCGTCAAGCAAATTCAGGCATTGCTGGCGAATCAGGTTCGTTCTCAGGATAT
CTGCATTACTGCGCGTACTAAACATGCTTGTGACCGCTACGCATCAGCACTGAATGACGCCGGAATTGAGACATTTAATT
TAGGCAATGATAGCGGTGACAGCGATGCACGCCCTGGCGTGCGGGTTGCAACGATGCACCGTATTAAAGGGCTTGAGTTC
CAGTATGTGTTCCTGGTGGGGATTAACGAAGGCGTTGTGCCGGAAATAAAAGCATTAGCATCCGATGATCCCGTTGAACA
GCGTGATGCATTATTTAATGAGCGCGCGTTACTGCATGTGGCTGCAACCCGTGCCGTAAAAGGGCTGTTTGTTTCATCAT
CTGGTGTCCCTAGTCCGCTATTAGTGGCTGACTGA

Upstream 100 bases:

>100_bases
ATAATCAAGAATTTATTCCATTATTCACAACCAGGGGTTGGCACGTTGCATTCGGGCCAATTGACGAAAATACATTGCAA
CATCTGTCCGGAGGTGACAA

Downstream 100 bases:

>100_bases
TAATATGTCCAAAACAGGCCAGGAGCCGAAACGCTCCTGGTCTATATAACCGTTCTGGCAACTGGCGATATTTATATTGC
TTAGCTCCCAACAGCCAGAC

Product: ATP-dependent DNA helicase, UvrD/REP family

Products: NA

Alternate protein names: DNA Helicase; LexA Repressor; UvrD/Rep Helicase; DNA Helicase II; NERD Domain-Containing Protein; Superfamily I DNA/RNA Helicase; Helicase; Plasmid Stabilization System; Superfamily I DNA/RNA Helicase-Like Protein; UvrD/REP Helicase Domain Protein; NERD Domain Protein; Superfamily Protein I DNA/RNA Helicase; Nerd Domain Protein; Peptidase; Superfamily I DNA And RNA Helicase-Like Protein; UvrD/REP Type DNA Helicase; ATP-Dependent DNA Helicase UvrD/REP Family; UvrD/REP Family ATP-Dependent DNA Helicase; Uvrd/Rep Helicase; NA Helicase.; DNA Helicase II-Like Protein; DNA/RNA Helicase Superfamily I

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNGLNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALY
MLMWVDKHDEAYDWARRHDCSIHPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHVVENEQELLQMLNAPLEKWRVFL
HPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARWLSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDV
VNIDAWMSDQLKRHGYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKVSRVGRGTA
LSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSSIVVDEAQDIGAPAFTLIRSLVPESPNDLFI
VGDGHQRIYRNKVVLGQCGINVRGRRSKKLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHA
ADFKEEAATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDARPGVRVATMHRIKGLEF
QYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAATRAVKGLFVSSSGVPSPLLVAD

Sequences:

>Translated_704_residues
MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNGLNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALY
MLMWVDKHDEAYDWARRHDCSIHPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHVVENEQELLQMLNAPLEKWRVFL
HPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARWLSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDV
VNIDAWMSDQLKRHGYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKVSRVGRGTA
LSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSSIVVDEAQDIGAPAFTLIRSLVPESPNDLFI
VGDGHQRIYRNKVVLGQCGINVRGRRSKKLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHA
ADFKEEAATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDARPGVRVATMHRIKGLEF
QYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAATRAVKGLFVSSSGVPSPLLVAD
>Mature_703_residues
TVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNGLNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYM
LMWVDKHDEAYDWARRHDCSIHPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAGL
NQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHVVENEQELLQMLNAPLEKWRVFLH
PSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARWLSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVV
NIDAWMSDQLKRHGYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKVSRVGRGTAL
SRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSSIVVDEAQDIGAPAFTLIRSLVPESPNDLFIV
GDGHQRIYRNKVVLGQCGINVRGRRSKKLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAA
DFKEEAATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDARPGVRVATMHRIKGLEFQ
YVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAATRAVKGLFVSSSGVPSPLLVAD

Specific function: Rep Helicase Is A Single-Stranded DNA-Dependent ATPase Involved In DNA Replication; It Can Initiate Unwinding At A Nick In The DNA. It Binds To The Single-Stranded DNA And Acts In A Progressive Fashion Along The DNA In The 3' To 5' Direction. [C]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.6.1.- [C]

Molecular weight: Translated: 79380; Mature: 79249

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNGLNYEKIHDARSKNVHS
CCCCCCCEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
VRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSIHPVTGAIQVIDISYIKPA
EEECCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCEEECCCEEEEEEEHHCCHH
AETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAGLNQLESIMPAEAWEPLHWLA
HHHHHCCCHHEECCCHHHHEEECCCHHHHHHHHHHHHHCCHHHHHHHCCHHHCCHHHHHH
EGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHVVENEQELLQMLNAPLEKWRVFL
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCHHHCEEEE
HPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARWLSQRLADKPGKKVLFTTFTRNLAA
CCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
DIRANLQRLCTREEMARIDVVNIDAWMSDQLKRHGYDFRVVYDSDEGRRKCWNYALQQAP
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCC
AELGLPDNFYAEEWQRVVQPNAVYTREEYLKVSRVGRGTALSRIQRAKIWPVFEEFRAQM
HHCCCCCCHHHHHHHHHHCCCCEECHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHH
ARAKLREMGDAMHEAIVLFKEKQVQLPYSSIVVDEAQDIGAPAFTLIRSLVPESPNDLFI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECHHHCCCHHHHHHHHHCCCCCCCEEE
VGDGHQRIYRNKVVLGQCGINVRGRRSKKLKINYRTTEETRQFAVGLLTGVKVDNLDGEA
EECCHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCC
DTSNDYLSLLHGEKPMITHAADFKEEAATLVKQIQALLANQVRSQDICITARTKHACDRY
CCCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHH
ASALNDAGIETFNLGNDSGDSDARPGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALA
HHHHHHCCCEEEECCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCHHHHHHC
SDDPVEQRDALFNERALLHVAATRAVKGLFVSSSGVPSPLLVAD
CCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCEECC
>Mature Secondary Structure 
TVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNGLNYEKIHDARSKNVHS
CCCCCCEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEE
VRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSIHPVTGAIQVIDISYIKPA
EEECCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCEEECCCEEEEEEEHHCCHH
AETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAGLNQLESIMPAEAWEPLHWLA
HHHHHCCCHHEECCCHHHHEEECCCHHHHHHHHHHHHHCCHHHHHHHCCHHHCCHHHHHH
EGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHVVENEQELLQMLNAPLEKWRVFL
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCHHHCEEEE
HPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARWLSQRLADKPGKKVLFTTFTRNLAA
CCCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHH
DIRANLQRLCTREEMARIDVVNIDAWMSDQLKRHGYDFRVVYDSDEGRRKCWNYALQQAP
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHCC
AELGLPDNFYAEEWQRVVQPNAVYTREEYLKVSRVGRGTALSRIQRAKIWPVFEEFRAQM
HHCCCCCCHHHHHHHHHHCCCCEECHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHH
ARAKLREMGDAMHEAIVLFKEKQVQLPYSSIVVDEAQDIGAPAFTLIRSLVPESPNDLFI
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECHHHCCCHHHHHHHHHCCCCCCCEEE
VGDGHQRIYRNKVVLGQCGINVRGRRSKKLKINYRTTEETRQFAVGLLTGVKVDNLDGEA
EECCHHHHHHCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCCC
DTSNDYLSLLHGEKPMITHAADFKEEAATLVKQIQALLANQVRSQDICITARTKHACDRY
CCCCHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHH
ASALNDAGIETFNLGNDSGDSDARPGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALA
HHHHHHCCCEEEECCCCCCCCCCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCHHHHHHC
SDDPVEQRDALFNERALLHVAATRAVKGLFVSSSGVPSPLLVAD
CCCCHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA