Click here to switch to the map view.

The map label for this gene is rbsR

Identifier: 209400180

GI number: 209400180

Start: 4834766

End: 4835758

Strand: Direct

Name: rbsR

Synonym: ECH74115_5190

Alternate gene names: 209400180

Gene position: 4834766-4835758 (Clockwise)

Preceding gene: 209398689

Following gene: 209397878

Centisome position: 86.77

GC content: 50.45

Gene sequence:

>993_bases
TTGGCTACAATGAAAGATGTTGCCCGCCTGGCGGGCGTTTCTACCTCAACAGTTTCTCACGTTATCAATAAAGATCGCTT
CGTCAGTGAAGCGATTACCGCCAAAGTTGAAGCGGCGATTAAAGAACTCAATTACGCGCCATCAGCTCTGGCGCGTAGCC
TCAAACTCAATCAAACACATACCATTGGCATGTTGATCACTGCCAGTACCAATCCTTTCTATTCAGAACTGGTGCGTGGC
GTTGAACGCAGCTGCTTCGAACGCGGTTATAGTCTCGTCCTTTGCAATACCGAAGGCGATGAACAGCGGATGAATCGCAA
TCTGGAAACGCTGATGCAAAAACGCGTTGATGGCTTGCTGTTACTGTGCACCGAAACGCATCAACCTTCGCGTGAAATCA
TGCAACGTTATCCGACAGTGCCTACTGTGATGATGGACTGGGCTCCGTTCGATGGCGACAGCGATCTTATTCAGGATAAC
TCGTTGCTGGGCGGAGACTTAGCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCT
GGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCT
ATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAG
GCCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGA
TATCGCGGTGATTGGCTATGACGATATCGAACTGGCAAGCTTTATGACGCCACCATTAACCACGATCCATCAACCAAAAG
ATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTT
ACTCCGATTCTGATGGAACGTGGTTCGGCTTAG

Upstream 100 bases:

>100_bases
CCATGCTGCCGCTGCGATTGCCGTAACACGTAAAGGCGCACAACCTTCCGTACCGTGGCGTGAAGAGATCGACGCATTTT
TAGACAGGCAGAGGTGACGC

Downstream 100 bases:

>100_bases
ATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATA
ATGCCCATTGTGATAAACGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQL
TPILMERGSA

Sequences:

>Translated_330_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQL
TPILMERGSA
>Mature_329_residues
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNS
LLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQA
VFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLT
PILMERGSA

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=100, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=46.969696969697, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.5987055016181, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=33.4470989761092, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=30.4075235109718, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.5483870967742, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788474, Length=305, Percent_Identity=29.5081967213115, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=27.6872964169381, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=28.6585365853659, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=25.8461538461538, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=27.5862068965517, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=26.2987012987013, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789456, Length=317, Percent_Identity=26.813880126183, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790192, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=24.1590214067278, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBSR_ECO57 (P0ACQ2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91215
- PIR:   H86061
- RefSeq:   NP_290392.1
- RefSeq:   NP_312722.1
- ProteinModelPortal:   P0ACQ2
- SMR:   P0ACQ2
- EnsemblBacteria:   EBESCT00000027373
- EnsemblBacteria:   EBESCT00000058050
- GeneID:   915322
- GeneID:   960413
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5254
- KEGG:   ecs:ECs4695
- GeneTree:   EBGT00050000009237
- HOGENOM:   HBG753640
- OMA:   IMQRYPS
- ProtClustDB:   PRK10423
- BioCyc:   ECOL83334:ECS4695-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36612; Mature: 36481

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPTLQQQRLQLTPILMERGSA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELNYAPSALARSLKLNQTH
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDNSLLGGDLATQYLIDKGHTRI
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTGDFEFNGGFDAMRQLLSHPLRPQ
EEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
AVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDIELASFMTPPLTTIHQPKDELGELA
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
IDVLIHRITQPTLQQQRLQLTPILMERGSA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796