Click here to switch to the map view.

The map label for this gene is menB [H]

Identifier: 209399870

GI number: 209399870

Start: 3147837

End: 3148694

Strand: Reverse

Name: menB [H]

Synonym: ECH74115_3404

Alternate gene names: 209399870

Gene position: 3148694-3147837 (Counterclockwise)

Preceding gene: 209400623

Following gene: 209397997

Centisome position: 56.51

GC content: 54.78

Gene sequence:

>858_bases
ATGATTTATCCTGATGAAGCAATGCTTTACGCACCGGTTGAATGGCACGACTGCTCCGAAGGCTTTGAGGACATTCGTTA
TGAAAAATCCACCGACGGTATCGCAAAAATCACCATTAATCGTCCGCAGGTGCGCAATGCCTTCCGTCCTCTGACGGTAA
AAGAGATGATCCAGGCACTGGCAGATGCGCGTTATGACGATAACGTCGGCGTGATCATTCTGACTGGCGCAGGCGATAAA
GCGTTCTGCTCCGGTGGTGACCAGAAAGTGCGTGGTGATTACGGCGGCTATAAAGATGATTCCGGCGTACATCACCTGAA
CGTGCTGGACTTCCAGCGTCAGATCCGTACCTGTCCGAAACCGGTTGTCGCGATGGTGGCTGGCTATTCCATCGGCGGTG
GTCACGTTCTGCACATGATGTGCGACCTGACTATCGCGGCAGATAATGCCATCTTCGGTCAGACTGGCCCGAAAGTCGGT
TCCTTCGACGGCGGCTGGGGCGCTTCCTACATGGCTCGTATCGTCGGGCAGAAAAAAGCGCGTGAAATCTGGTTCCTGTG
CCGTCAGTACGACGCAAAACAGGCGCTGGATATGGGCCTTGTCAACACCGTGGTACCGCTGGCGGATCTGGAAAAAGAAA
CCGTTCGCTGGTGCCGTGAAATGCTGCAAAACAGTCCGATGGCGCTGCGCTGCCTGAAAGCTGCACTGAACGCCGACTGT
GACGGTCAGGCAGGTCTGCAGGAGCTGGCGGGCAACGCCACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCG
CAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAA

Upstream 100 bases:

>100_bases
ATGTCATTCCTCGCGCCGGACATAACGCGCATCGGGAAAATCCCGCTGGCGTAATCGCAAGTCTGGCGCAGATCTTGCGT
TTCTGACTAAAGGACACAAT

Downstream 100 bases:

>100_bases
TGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGC
GACGGGCTGTACGTTTGCCT

Product: naphthoate synthase

Products: NA

Alternate protein names: DHNA-CoA synthase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP

Sequences:

>Translated_285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
>Mature_285_residues
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDK
AFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP

Specific function: Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA) [H]

COG id: COG0447

COG function: function code H; Dihydroxynaphthoic acid synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=271, Percent_Identity=32.8413284132841, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=30.952380952381, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=30, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=240, Percent_Identity=27.5, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594469, Length=142, Percent_Identity=28.8732394366197, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=142, Percent_Identity=28.8732394366197, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI25453481, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538573, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757966, Length=235, Percent_Identity=24.6808510638298, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538581, Length=235, Percent_Identity=24.6808510638298, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757968, Length=235, Percent_Identity=25.1063829787234, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=235, Percent_Identity=25.1063829787234, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=285, Percent_Identity=99.6491228070175, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=27.2373540856031, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI221142681, Length=248, Percent_Identity=29.4354838709677, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=29.1828793774319, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=229, Percent_Identity=24.8908296943231, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=30.5660377358491, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=241, Percent_Identity=26.1410788381743, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=27.3062730627306, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=197, Percent_Identity=24.8730964467005, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=223, Percent_Identity=24.2152466367713, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=32.7067669172932, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=32.7067669172932, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653139, Length=278, Percent_Identity=26.978417266187, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=28.6259541984733, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=28.804347826087, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=178, Percent_Identity=30.8988764044944, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376
- InterPro:   IPR010198 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.1.3.36 [H]

Molecular weight: Translated: 31619; Mature: 31619

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQAL
CCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHH
ADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPK
HCCCCCCCCCEEEEECCCCCHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCH
PVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVGSFDGGWGASYMARIVGQKKA
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
REIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
CCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQAL
CCCCCCCEEEECCCHHCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHH
ADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPK
HCCCCCCCCCEEEEECCCCCHHHCCCCCEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCH
PVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVGSFDGGWGASYMARIVGQKKA
HHHHHHHCCCCCCHHHHHHHHHEEEECCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
REIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
CCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]