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The map label for this gene is apbE

Identifier: 209399737

GI number: 209399737

Start: 3084828

End: 3085883

Strand: Reverse

Name: apbE

Synonym: ECH74115_3352

Alternate gene names: 209399737

Gene position: 3085883-3084828 (Counterclockwise)

Preceding gene: 209400412

Following gene: 209398796

Centisome position: 55.38

GC content: 53.22

Gene sequence:

>1056_bases
ATGGAAATAAGCTTTACCCGCGTGGCACTGCTGGCTGCCGCGCTCTTCTTTGTTGGTTGCGATCAAAAACCACAACCCGC
CAAAACCCACGCTACTGAAGTTACCGTTCTTGAAGGCAAAACTATGGGTACCTTCTGGCGTGCCAGCATCCCGGGCATTG
ACGCCAAACGCAGCGCCGAACTTAAAGAAAAGATTCAGACCCAGCTGGACGCCGACGATCAGCTGCTTTCGACCTATAAA
AAAGATTCCGCGCTGATGCGCTTTAACGACTCGCAAAGTTTATCGCCGTGGCCGGTAAGTGAAGCGATGGCCGATATCGT
CACCACCTCGCTACGCATTGGCGCGAAGACCGATGGCGCGATGGATATAACCGTCGGGCCGCTGGTGAATCTGTGGGGTT
TCGGTCCGGAACAACAGCCGGTTCAAATTCCGAGCCAGGAACAGATCGACGCGATGAAAGCCAAAACTGGTTTACAGCAC
CTTACGGTCATTAATCAGTCGCATCAGCAATATCTGCAAAAAGACCTGCCGGATTTATATGTCGATCTCTCCACCGTCGG
CGAAGGTTATGCGGCGGATCACCTGGCACGCTTGATGGAGCAGGAAGGGATTTCCCGCTATCTGGTGTCGGTGGGCGGCG
CGCTGAACAGCCGTGGTATGAACGGTGAAGGCCTGCCGTGGCGGGTAGCGATTCAAAAACCAACCGATAAAGAAAACGCG
GTTCAGGCCGTGGTGGATATCAACGGTCATGGGATTAGCACCTCTGGCAGCTACCGTAACTATTACGAACTGGACGGCAA
ACGTCTTTCCCATGTTATCGATCCGCAAACCGGGCGTCCCATCGAACACAATCTGGTATCCGTGACGGTGATTGCACCGA
CGGCGCTGGAAGCCGACGCCTGGGATACAGGTTTGATGGTACTCGGGCCGGAGAAAGCCAAAGAAGTTGTTCGCCGGGAA
GGGCTGGCGGTTTATATGATCACCAAAGAAGGCGATAGCTTTAAAACCTGGATGTCACCACAGTTTAAAAGCTTCCTTGT
CAGCGAAAAAAATTAA

Upstream 100 bases:

>100_bases
ATCGAACAAAGGGCCTGCGGGCCCTTTTTTCATTGTTTTCAGCGTACAAACTCAGTTTTTTGGTGTACTCTTGCGACCGT
TCGCATGAGGATAATCACGT

Downstream 100 bases:

>100_bases
AGCGCAAGATTGTTGGTTTTTGCGTGATGGTGGCCGGGCAGACTAAAGGCTATCCTTAACCAGGGAGCTGATTATGAAAA
ACGCCACATGCTTAACTGAC

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Sequences:

>Translated_351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
>Mature_351_residues
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYK
KDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRRE
GLAVYMITKEGDSFKTWMSPQFKSFLVSEKN

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family

Homologues:

Organism=Escherichia coli, GI1788543, Length=351, Percent_Identity=100, Blast_Score=726, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APBE_ECO57 (P0AB86)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85861
- PIR:   G91016
- RefSeq:   NP_288794.1
- RefSeq:   NP_311130.1
- ProteinModelPortal:   P0AB86
- SMR:   P0AB86
- EnsemblBacteria:   EBESCT00000028907
- EnsemblBacteria:   EBESCT00000060104
- GeneID:   916810
- GeneID:   957195
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3472
- KEGG:   ecs:ECs3103
- GeneTree:   EBGT00050000010581
- HOGENOM:   HBG292712
- OMA:   MAEIVTT
- ProtClustDB:   PRK10461
- BioCyc:   ECOL83334:ECS3103-MONOMER
- InterPro:   IPR003374

Pfam domain/function: PF02424 ApbE

EC number: NA

Molecular weight: Translated: 38549; Mature: 38549

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHH
LKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGA
HHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCCCE
MDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQHLTVINQSHQQYLQKDLPDLY
EEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHE
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC
>Mature Secondary Structure
MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGTFWRASIPGIDAKRSAE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEECCCCCCCCHHHHH
LKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEAMADIVTTSLRIGAKTDGA
HHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCCCE
MDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQHLTVINQSHQQYLQKDLPDLY
EEEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHE
VDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSRGMNGEGLPWRVAIQKPTDKENA
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHCC
VQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQTGRPIEHNLVSVTVIAPTALEADA
EEEEEEECCCCCCCCCCCCCEEECCCHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
WDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSFKTWMSPQFKSFLVSEKN
CCCCEEEECCHHHHHHHHHCCCEEEEEECCCCCCHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796