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The map label for this gene is spaS [H]

Identifier: 209399671

GI number: 209399671

Start: 3827232

End: 3828353

Strand: Reverse

Name: spaS [H]

Synonym: ECH74115_4137

Alternate gene names: 209399671

Gene position: 3828353-3827232 (Counterclockwise)

Preceding gene: 209399840

Following gene: 209396063

Centisome position: 68.71

GC content: 33.07

Gene sequence:

>1122_bases
ATGGCAAATAAAACTGAAAAGCCAACACAAAAGAAACTGCAGGATGCTTCTAAAAAAGGGCAAATTCTAAAAAGTAGAGA
CTTAACCGTTTCTGTAATAATGCTTGTGGGTACTTTATATCTCGGATATGTTTTTGATGTGCATCACATTATGTCGATTC
TTGAATATATCCTTGATCATAACGCTAAACCGGATATTTGGGACTATTTTAAAGCTATGGGGATTGGTTGGTTGAAAACG
ATCATTCCTTTTTTGCTGGTTTGCATGTTCACAACAATACTTGTCTCCTGGTTTCAAAGTAAAATGCAATTAGCAACTGA
AGCTGTAAAATTAAAGTTTGATTCATTGAATCCAGTAAATGGTTTAAAGCGTATATTTGGCTTAAAAACCGTAAAAGAAT
TTGTTAAAGCAATTCTTTATATTATTTTTTTTGCATTGGAGATCAAAGTATTTTGGAGTAATCATAAATCACTGCTTTTT
AAAACTCTTGATGGAGATATCATATCTTTATTATCAGATTGGGGAGAGATGCTATTCCTTCTCATACTGTATTGTCTCGG
CAGTATGATAATTGTCTTAATTTTTGATTTTATCGCTGAATATTTTTTATTTATGAAAGATATGAAAATGGATAAACAAG
AAGTTAAAAGAGAATACAAGGAACAAGAAGGAAATCCTGAAATTAAGTCTAAACGCAGAGAGCGCCATCAGGAAATTCTT
TCTGAGCAATTGAAATCTGATGTCAGTAATAGCCGTTTGATGATTGCCAACCCTACTCACATTGCAATAGGGATATACTT
TAAGCCACATCTGTCACCTATTCCATTGATTTCTGTAAGAGAAACTAATGAGGTAGCATTAGCTGTAAGGAAATATGCAA
AGGAAATCGGGATACCAATTATTACAGATAAAAAATTAGCACGAAAAATTTATGCTACCCATCGTCGCTACGATTATGTT
AGCTTCGAAAATATAGATGAAATATTACGTCTTCTGCTGTGGCTTGAAGATGTGGAGAATGCTGGACAACCTGTTCCAGA
TGAACAGCTCTCTTCAGAAGATAAATATATTGAGGGTGAAGACACAAAAAGCGAGAATAATGACAATAATTTAAAAAATT
AA

Upstream 100 bases:

>100_bases
TATTAATTATTTATTTTGGCCCAATACTGGCTGAACGAGTGATGCCGTTATCCTTTTTCCCAGAACAATTGCAACTATAC
ATTGAGAAATAGAGTGTTTC

Downstream 100 bases:

>100_bases
ACATGTATCGTAGTCAAAAATTTATAAGAATCTACACGCAAAAAACGTGTAGATTTTTATTTAATGAGTTAAACGATCTT
GCGAAATGACAAGCAATTGA

Product: surface presentation of antigens protein SpaS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKT
IIPFLLVCMFTTILVSWFQSKMQLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLF
KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEIL
SEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYV
SFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLKN

Sequences:

>Translated_373_residues
MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKT
IIPFLLVCMFTTILVSWFQSKMQLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLF
KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEIL
SEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYV
SFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLKN
>Mature_372_residues
ANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYVFDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTI
IPFLLVCMFTTILVSWFQSKMQLATEAVKLKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLFK
TLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEILS
EQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVS
FENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGEDTKSENNDNNLKN

Specific function: Involved in a secretory pathway responsible for the surface presentation of determinants needed for the entry of Salmonella species into mammalian cells [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=320, Percent_Identity=25.625, Blast_Score=121, Evalue=9e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 43343; Mature: 43212

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCC
>Mature Secondary Structure 
ANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYVFDVHHIMSILEYILDH
CCCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKMQLATEAVKLKFDSLNPVN
CCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
GLKRIFGLKTVKEFVKAILYIIFFALEIKVFWSNHKSLLFKTLDGDIISLLSDWGEMLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHH
LILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERHQEIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIGIPI
HHHHHHHHCCCEEEEECCCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCCCE
ITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDKYIEGE
ECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCEECCC
DTKSENNDNNLKN
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8404849; 11677609 [H]