Click here to switch to the map view.

The map label for this gene is tnaB

Identifier: 209399599

GI number: 209399599

Start: 4782040

End: 4783287

Strand: Direct

Name: tnaB

Synonym: ECH74115_5140

Alternate gene names: 209399599

Gene position: 4782040-4783287 (Clockwise)

Preceding gene: 209397514

Following gene: 209395703

Centisome position: 85.82

GC content: 46.71

Gene sequence:

>1248_bases
ATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATCGGTGGAGGTAT
GTTTGCTTTACCTGTGGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCCTTTATCCTTATCATTGCCTGGTTTTCAATGC
TTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCCGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTA
ATCGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGC
TAATGGTGCGATCATTAGTGAAACGATATCAATGAATTTGGGTTATCACGCTAATCCACGTATTGTCGGGATCTGCACAG
CCATTTTCGTTGCCAGCGTATTGTGGATAAGCTCGTTAGCCGCCAGTCGTATTACCTCATTGTTCCTCGGGCTGAAGATT
ATCTCCTTTGTGATCGTGTTTGGTTCTTTCTTCTTCCTGGTCGATTACTCCATTCTGCGCGATGCCACCAGCTCCACTGC
GGGAACGTCTTACTTCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCA
GCCTGATTATTTGCTATGGCAAACGCAAAGATAAGCTAATCAAAAGCGTGGTCTTCGGTTCGCTGCTGGCGCTGGTGATT
TATCTCTTCTGGCTCTATTGCACCATGGGGAATATTCCGCGAGAAAGCTTTAAGGCGATTATTTCCTCAGGCGGCAACGT
TGATTCACTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCCAACTTAG
CTGTCGCCAGCTCGTTTTTTGGTGTCACGCTGGGATTGTTCGATTATCTGGCGGACCTGTTTAAGATTGATAACTCCCAC
GGCGGGCGTTTCAAAACCGTGCTGTTAACCTTCCTGCCACCCGCGTTGTTGTATCTGATCTTCCCGAACGGCTTTATTTA
CGGGATCGGCGGTGCCGGACTGTGCGCCACTATTTGGGCGGTCATTATTCCCGCAGTGCTGGCAATCAAAGCTCGCAAAA
AGTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTAATT
TTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAA

Upstream 100 bases:

>100_bases
AGAAGTTTAATTAATACTACTGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGC
CTTCCTCTAAAGGTGGCATC

Downstream 100 bases:

>100_bases
ATCCTTCAAGAAGCCAGTCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCC
TCCTTCACCACGTATGCTTT

Product: tryptophan permease TnaB

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG

Sequences:

>Translated_415_residues
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG
>Mature_414_residues
TDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLI
GNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKII
SFVIVFGSFFFLVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIY
LFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHG
GRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVIL
CWFGNVFNVLPKFG

Specific function: Involved in tryptophan transport across the cytoplasmic membrane. Plays a role in transporting tryptophan which is to be used catabolically

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily

Homologues:

Organism=Escherichia coli, GI1790145, Length=415, Percent_Identity=99.5180722891566, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1789552, Length=388, Percent_Identity=53.0927835051546, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1788218, Length=410, Percent_Identity=32.9268292682927, Blast_Score=194, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TNAB_ECO57 (Q8XB33)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86056
- PIR:   F91209
- RefSeq:   NP_290345.1
- RefSeq:   NP_312673.1
- ProteinModelPortal:   Q8XB33
- EnsemblBacteria:   EBESCT00000026366
- EnsemblBacteria:   EBESCT00000059533
- GeneID:   915392
- GeneID:   960741
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5204
- KEGG:   ecs:ECs4646
- GeneTree:   EBGT00050000009663
- HOGENOM:   HBG482321
- OMA:   ICYGKKK
- ProtClustDB:   PRK09664
- BioCyc:   ECOL83334:ECS4646-MONOMER
- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227
- PRINTS:   PR00166
- TIGRFAMs:   TIGR00837

Pfam domain/function: PF03222 Trp_Tyr_perm

EC number: NA

Molecular weight: Translated: 45196; Mature: 45065

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00594 AROMATIC_AA_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12d9dc0c)-; HASH(0x129350e8)-; HASH(0x12b57760)-; HASH(0x1258a2b4)-; HASH(0x12d22b70)-; HASH(0x12de00bc)-; HASH(0x1142f2cc)-; HASH(0x1289dbb4)-; HASH(0x1255f8b8)-; HASH(0x1295f020)-; HASH(0x10e344fc)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFLVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
TDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFLVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796