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The map label for this gene is maiA [H]

Identifier: 209399225

GI number: 209399225

Start: 3002047

End: 3002691

Strand: Reverse

Name: maiA [H]

Synonym: ECH74115_3268

Alternate gene names: 209399225

Gene position: 3002691-3002047 (Counterclockwise)

Preceding gene: 209400558

Following gene: 209397299

Centisome position: 53.89

GC content: 54.26

Gene sequence:

>645_bases
ATGAAGCTGTACAGTTTTTTTAATAGTTCAGCGTCGTACCGGGTACGCATTGCGCTGGCGCTGAAAGGCATTAATTACCA
GACCGAAGGGGTGAATATCCGTATTGGTCAGCAAAATGAACTGGCCTATCGGCGGATGAATCCGGTCGGGCTGGTGCCGA
CGCTGCTGACTGACGAGGGCGAATCACTCGGACAATCGCTGGCGATTATTGACTGGCTGGAGAGGCACTACCCGCAAGTT
CCGCTGGTGCCGCAGGAAGAGCCGGCCCGCAACAAGGTGCTGGAAATTGTCTATGCCATCGCTTGTGATATTCATCCATT
GAATAACTTGCGCGTGCTGCGCTACCTGACCGAAGAACTGAACGTCAGTGAAGAAGAAAAAAAACGTTGGTATGCGCACT
GGATCCAGCAAGGGTTAAGCGCGGTGGAACAACTGTTGCGCCAGAACCAGTCGGGGCAATTTTGTGTGGGCGAGACGCCG
ACGCTGGCAGATTGCTGCCTTGTCCCGCAGTGGGCGAACGCGCTACGCATGAACTGCGATTTGAGCGGGTATCCACGCTG
CAAAGCGGTGTACGACGCCTGCACACAGCTGCCAGCGTTCATTGCCGCAGCGCCAGAAAATCAACAAGATAAAATTTCAG
CCTGA

Upstream 100 bases:

>100_bases
GTACGCCGGAAGGGGTTGGCCCGGTAGTGAAAGGTGATGTGATCACAGGCAACGTCGAGGGCTTAACGCCTATCGCGGTG
AAGATTGTCTGAGGTGCATG

Downstream 100 bases:

>100_bases
CCAGGAGAACTATAATGACGAAAGTGACACGCGCAGTAATTGTGGGAGGCGGGATCGGCGGTGCGGCAACTGCGCTGTCA
CTGGCCCGCCTGGGGATCAA

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA

Sequences:

>Translated_214_residues
MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
>Mature_214_residues
MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQV
PLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=43.8095238095238, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=41.5094339622642, Blast_Score=103, Evalue=8e-23,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=35.2380952380952, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=38.4615384615385, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=37.3205741626794, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=34.9282296650718, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=44.7619047619048, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=44.7619047619048, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=44.7619047619048, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=38.8625592417062, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 24241; Mature: 24241

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEG
CCCCEECCCCCCEEEEEEEEEECCCEEECCEEEEECCCCCHHHHHCCCCCCCHHHCCCCC
ESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
NVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETPTLADCCLVPQWANALRMNCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCHHHHHHEEECCC
LSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYRRMNPVGLVPTLLTDEG
CCCCEECCCCCCEEEEEEEEEECCCEEECCEEEEECCCCCHHHHHCCCCCCCHHHCCCCC
ESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLTEEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
NVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETPTLADCCLVPQWANALRMNCD
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHCCHHHHHHEEECCC
LSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]