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The map label for this gene is dnaB [H]

Identifier: 209398833

GI number: 209398833

Start: 2704660

End: 2706036

Strand: Reverse

Name: dnaB [H]

Synonym: ECH74115_2920

Alternate gene names: 209398833

Gene position: 2706036-2704660 (Counterclockwise)

Preceding gene: 209396675

Following gene: 209400775

Centisome position: 48.56

GC content: 52.29

Gene sequence:

>1377_bases
ATGAGACAGGATATCGAAGCTAGCGTTATCGGTGGCCTGCTGATTGGTGGATTAACTCCAACCGCCAGTGACGTTCTGGC
AACGCTGGAGCCGGAAGCGTTTTCAATTCCGCTCTACCGGAAAGCCTTCGAGGTTATTCGCAAGCAGGCGAGAAACAGAA
ACCTAATCGATGCGCTGATGGTTGCCGAGGCGTGCGGAGAGGAGCATTTCACGTCAATCCTGATGACCAGCAAAAACTGC
CCGAGTGCCGCAAACCTGAAGGGATATGCCGGAATGGTCGCGGATAACTATCACCGCCGTCTGGTGCTGGAAATCATGGA
TGAAATGCGTGAACCAATCCAAAGCGGAACCATCGACGCATCGAGTCAGGCGATGGATGAACTTGTAAAGCGTCTTTCAG
CCATCAGAAAGCCCCGTGACGAGGTTAAACCTGTACGGTTAGGGGAAATCATCACTGACTACACTGACACGCTTGACAGG
CGTCTGAGGAACGGAGAAGAGTCAGATACCCTGAAGACCGGAATCGAAGAGCTTGACGCTATCACTGGAGGGATGAACGC
AGAAGACCTGGTGATTATCGCCGCTCGTCCTGGTATGGGGAAAACCGAACTGGCGCTGAAGATTGCCGAAGGTGTGGCAA
GCCGCGTTATTCCTGGTTCTGACGTCCGGCGCGGGGTATTGATTTTCTCAATGGAAATGAGCGCATTGCAGATTGCAGAG
CGAAGCATTGCCAACGCCGGGAGGATGTCGGTTAGCGTACTGCGAAATCCTGCATCGATGGATGACGAGGGCTGGGCACG
TGTTGCTAACGGCATGAGTCAGCTTGCAGATTTGGATGTATGGGTAGTCGATGCTTCGCGGTTATCGGTCGAAGAAATAC
GCTCAATCGCAGAACGGCATAAGCAGGAAAATCCAAACCTGTCACTCATCATGGCGGATTATCTTGGCCTGATTGAGAAG
CCGAAAGCAGACCGCAACGACCTCGCAATTGCTCACATCTCCGGAAGCCTGAAGGCGATGGCGAAAGACCTGAAAACACC
GGTTATCTCCCTGAGTCAGCTTTCGCGCGATGTTGAGAAGCGACCAAACAAACGCCCGACAAACGCAGATTTGCGTGATT
CAGGAAGCATTGAACAGGACGCAGACTCAATCATCATGCTCTATCGGGAAGCGGTATATGATGAGAACAGTAGCGCCGCG
CCATTTGCTGAAATCATCGTGACGAAAAACCGTTTTGGCTCGCTTGGTACAGTTTACCAGCGGTTCTGTAACGGACACTT
TGTTGCATGTGACCAGGATGAAGCCAGACAGATTTGCACAGCATCAAATGCACCTGCTGCGCGTGGCAGACGATATGCAC
AAGGGGCTGACGTATGA

Upstream 100 bases:

>100_bases
GAGACAACGACACGGGCTGGACGGCAGATTTTGATTACCTGCTGAGGGAAGATTCGTTAACGGGAGTTCGGGAAGGGAAG
TTTGCAGACAGGGGGATTGC

Downstream 100 bases:

>100_bases
CCATCTACATCACTGAGTTAATAACAGGCCTGCTGGTAATCGCAGGCCTTTTTATTTGGGGGAGAGGGAAGTGAACGATA
GCTACCGACAGTTTGAAAAC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGRRYAQGADV

Sequences:

>Translated_458_residues
MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGRRYAQGADV
>Mature_458_residues
MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNC
PSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEK
PKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAA
PFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGRRYAQGADV

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=35.2144469525959, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50151; Mature: 50151

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALM
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
VAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDA
HHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
SSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDRRLRNGEESDTLKTGIEELDA
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHH
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
KQENPNLSLIMADYLGLIEKPKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEK
HCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAAPFAEIIVTKNRFGSLGTVYQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHHHH
RFCNGHFVACDQDEARQICTASNAPAARGRRYAQGADV
HHHCCCEEEECHHHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFEVIRKQARNRNLIDALM
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHH
VAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLVLEIMDEMREPIQSGTIDA
HHHHHCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
SSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDRRLRNGEESDTLKTGIEELDA
HHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIPGSDVRRGVLIFSMEMSALQIAE
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHH
RSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLADLDVWVVDASRLSVEEIRSIAERH
HHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHH
KQENPNLSLIMADYLGLIEKPKADRNDLAIAHISGSLKAMAKDLKTPVISLSQLSRDVEK
HCCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHH
RPNKRPTNADLRDSGSIEQDADSIIMLYREAVYDENSSAAPFAEIIVTKNRFGSLGTVYQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCHHHHHH
RFCNGHFVACDQDEARQICTASNAPAARGRRYAQGADV
HHHCCCEEEECHHHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]