Click here to switch to the map view.

The map label for this gene is uraA

Identifier: 209398719

GI number: 209398719

Start: 3439131

End: 3440420

Strand: Reverse

Name: uraA

Synonym: ECH74115_3719

Alternate gene names: 209398719

Gene position: 3440420-3439131 (Counterclockwise)

Preceding gene: 209395970

Following gene: 209400783

Centisome position: 61.74

GC content: 52.48

Gene sequence:

>1290_bases
ATGACGCGCCGTGCTATCGGGGTGAGTGAAAGACCGCCACTTTTACAGACAATCCCGCTTAGTTTGCAACATTTGTTCGC
CATGTTTGGTGCAACCGTCCTGGTGCCCGTCTTATTTCATATTAACCCGGCGACTGTACTGTTATTTAACGGTATTGGAA
CGCTGCTGTATCTCTTCATCTGTAAAGGGAAAATTCCGGCTTATCTTGGTTCCAGCTTTGCCTTTATTTCACCGGTATTG
TTACTGTTGCCGTTAGGGTATGAAGTCGCGCTGGGCGGCTTTATTATGTGCGGCGTGCTGTTCTGCCTGGTTTCTTTTAT
CGTGAAGAAAGCGGGGACCGGCTGGCTGGACGTGCTGTTTCCACCTGCGGCAATGGGCGCAATCGTTGCCGTCATCGGTC
TGGAGCTGGCGGGCGTAGCTGCCGGTATGGCGGGTTTACTCCCGGCTGAAGGGCAAACGCCAGACTCCAAAACCATCATC
ATCTCTATTACCACCCTGGCGGTCACGGTTTTAGGTTCCGTGCTGTTCCGTGGTTTCCTGGCAATTATCCCGATTTTAAT
TGGCGTGCTGGTGGGGTACGCGCTCTCTTTCGCAATGGGAATTGTCGATACCACGCCGATTATTAATGCTCACTGGTTTG
CGCTGCCAACCCTCTATACGCCGCGCTTCGAGTGGTTTGCCATTCTGACTATTCTGCCAGCGGCGTTAGTGGTTATTGCC
GAACACGTAGGGCACCTGGTAGTAACGGCTAATATCGTCAAAAAAGATCTGCTGCGCGATCCAGGTCTGCACCGTTCGAT
GTTTGCTAACGGCTTGTCGACCGTGATCTCCGGCTTCTTTGGCTCCACGCCAAACACCACCTACGGGGAAAACATTGGCG
TGATGGCGATCACCCGTGTTTACAGTACCTGGGTTATCGGCGGGGCGGCGATTTTCGCTATCCTGCTTTCCTGCGTCGGT
AAACTGGCTGCCGCTATCCAGATGATCCCATTGCCGGTGATGGGCGGCGTTTCGCTGCTGCTTTATGGTGTCATCGGTGC
TTCCGGTATTCGTGTTTTGATCGAATCGAAAGTGGATTACAACAAAGCACAGAACCTGATCCTGACTTCCGTGATTTTGA
TCATCGGCGTCAGTGGCGCGAAGGTAAACATTGGCGCGGCGGAGCTGAAAGGTATGGCGCTGGCGACCATCGTCGGTATC
GGCTTAAGTTTGATTTTCAAACTGATTAGTGTGCTGCGCCCTGAAGAAGTGGTGCTGGACGCAGAAGACGCGGATATAAC
AGACAAATAA

Upstream 100 bases:

>100_bases
TTTGGTACGAAATAAAGAATAAAAATAATTAAAGCCGACTTTAAGAGTCGGCTTTTTTTTGAGTAAAGCGCCTATAACAC
ATAATACAGAGGATAATACT

Downstream 100 bases:

>100_bases
TGTACCGACCGGGCAGTGTTGCTGCCCGGTTCAAACATCATGGGATTCTGTGGTAAACTTCTCGCGATTTTGTGAAATCC
TGGTTGAGGTATCTCTGAAC

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVG
KLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGI
GLSLIFKLISVLRPEEVVLDAEDADITDK

Sequences:

>Translated_429_residues
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVL
LLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIA
EHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVG
KLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGI
GLSLIFKLISVLRPEEVVLDAEDADITDK
>Mature_428_residues
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLL
LLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIII
SITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIAE
HVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGK
LAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIG
LSLIFKLISVLRPEEVVLDAEDADITDK

Specific function: Transport of uracil in the cell

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily

Homologues:

Organism=Homo sapiens, GI44680148, Length=220, Percent_Identity=26.8181818181818, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI40316845, Length=220, Percent_Identity=26.8181818181818, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788843, Length=429, Percent_Identity=100, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI87081818, Length=425, Percent_Identity=36.9411764705882, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082181, Length=391, Percent_Identity=27.6214833759591, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790087, Length=444, Percent_Identity=25.6756756756757, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17541904, Length=223, Percent_Identity=25.5605381165919, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=216, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558856, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356175, Length=432, Percent_Identity=23.8425925925926, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): URAA_ECO57 (P0AGM8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85893
- PIR:   G91048
- RefSeq:   NP_289050.1
- RefSeq:   NP_311386.1
- ProteinModelPortal:   P0AGM8
- EnsemblBacteria:   EBESCT00000028131
- EnsemblBacteria:   EBESCT00000055705
- GeneID:   915224
- GeneID:   957761
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3760
- KEGG:   ecs:ECs3359
- GeneTree:   EBGT00050000009612
- HOGENOM:   HBG470187
- OMA:   NFFKDPG
- ProtClustDB:   PRK10720
- BioCyc:   ECOL83334:ECS3359-MONOMER
- InterPro:   IPR006042
- InterPro:   IPR006043
- PANTHER:   PTHR11119:SF3
- PANTHER:   PTHR11119
- TIGRFAMs:   TIGR00801

Pfam domain/function: PF00860 Xan_ur_permease

EC number: NA

Molecular weight: Translated: 45061; Mature: 44930

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf91aba8)-; HASH(0x102a1b18)-; HASH(0x10067b70)-; HASH(0xf547f90)-; HASH(0xfce7474)-; HASH(0xefc74a0)-; HASH(0x10368cec)-; HASH(0x1091a4b4)-; HASH(0x10924530)-; HASH(0xc0d83fc)-; HASH(0x10958644)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHH
CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLF
HCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
PPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIIISITTLAVTVLGSVLFRGFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
AIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
EHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV
HHHCHHEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
YSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCH
NKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIGLSLIFKLISVLRPEEVVLD
HHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AEDADITDK
CCCCCCCCH
>Mature Secondary Structure 
TRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPATVLLFNGIGTLLYLFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHHHH
CKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLF
HCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
PPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTIIISITTLAVTVLGSVLFRGFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
AIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTLYTPRFEWFAILTILPAALVVIA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
EHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV
HHHCHHEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
YSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCH
NKAQNLILTSVILIIGVSGAKVNIGAAELKGMALATIVGIGLSLIFKLISVLRPEEVVLD
HHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
AEDADITDK
CCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796