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The map label for this gene is lnt

Identifier: 209398690

GI number: 209398690

Start: 772779

End: 774317

Strand: Reverse

Name: lnt

Synonym: ECH74115_0749

Alternate gene names: 209398690

Gene position: 774317-772779 (Counterclockwise)

Preceding gene: 209398266

Following gene: 209399444

Centisome position: 13.9

GC content: 55.62

Gene sequence:

>1539_bases
ATGGCTTTTGCCTCATTAATTGAACGCCAGCGCATTCGCCTGCTGCTGGCGTTATTATTCGGTGCCTGCGGAACGCTGGC
CTTCTCTCCTTACGACGTCTGGCCTGCGGCGATTATTTCGCTGATGGGGCTTCAGGCGTTGACCTTTAACCGCCGTCCAC
TCCAGTCTGCCGCTATTGGCTTTTGCTGGGGATTTGGCCTCTTTGGCAGCGGTATTAACTGGGTCTATGTCAGCATCGCG
ACCTTTGGCGGAATGCCTGGCCCGGTTAACATCTTCCTGGTGGTACTGCTGGCGGCGTATTTGTCGCTGTATACCGGACT
GTTTGCTGGCGTGCTGTCGCGCCTGTGGCCGAAAACCACCTGGCTGCGCGTGGCGATTGCCGCCCCTGCTCTCTGGCAAG
TGACCGAGTTTCTGCGCGGTTGGGTACTGACAGGCTTCCCGTGGTTACAGTTCGGCTATAGCCAGATTGATGGCCCGTTA
AAAGGGCTGGCACCGCTAATGGGCGTGGAAGCCATTAACTTCCTGCTTATGATGGTTAGCGGCCTGCTGGCACTGGCGTT
AGTCAAACGCAACTGGCGTCCGCTGGTGGTGGCCGTCGTGCTGTTTGCCCTTCCCTTCCCGCTGCGTTACATCCGGTGGT
TTACCCCACAACCGGAGAAAACCATTCAGGTTTCGATGGTTCAGGGCGATATTCCGCAATCGCTGAAATGGGACGAAGGC
CAGCTTCTTAATACGCTGAAGATTTACTACAACGCAACGGCACCGCTGATGGGCAAATCATCGTTGATTATCTGGCCGGA
GTCGGCGATAACCGATCTGGAAATTAATCAGCAACCGTTCCTCAAAGCACTGGACGGTGAGTTGCGTGATAAAGGTAGCT
CGCTGGTGACCGGGATTGTTGACGCGCGTCTTAATAAGCAGAACCGTTACGATACCTACAACACCATCATCACACTGGGT
AAAGGTGCACCGTACAGCTACGAATCAGCCGATCGCTATAACAAAAACCATCTGGTGCCGTTTGGCGAGTTTGTCCCGCT
GGAGTCGATTCTGCGTCCGTTAGCACCGTTCTTTGATCTGCCGATGTCGTCGTTCAGCCGTGGGCCATATATCCAGCCGC
CGCTGTCGGCAAATGGTATTGAGCTTACTGCGGCTATTTGCTACGAGATCATTCTCGGCGAGCAAGTGCGCGATAACTTC
CGCCCGGATACCGACTATCTGCTGACTATCTCCAACGATGCGTGGTTTGGTAAGTCTATTGGTCCATGGCAGCACTTCCA
GATGGCGCGAATGCGTGCGCTGGAGCTGGCGCGCCCACTGTTGCGCAGCACCAACAACGGCATTACGGCGGTGATTGGCC
CGCAGGGTGAGATTCAGGCGATGATCCCGCAGTTCACCCGCGAGGTGTTAACCACTAACGTGACGCCGACCACCGGACTC
ACACCATACGCACGTACCGGCAACTGGCCGCTGTGGGTGCTGACGGCACTGTTTGGTTTTGCTGCTGTGTTGATGAGTCT
GCGTCAGCGACGTAAATAA

Upstream 100 bases:

>100_bases
GGCCGACAGTCGGCGTATTATTCAGGTTCATGTCAAAATCCCGGATGACTCACCCCAGCCGAAGCTGGATGAATAAAACC
GAAACTGGATAGATAACTAC

Downstream 100 bases:

>100_bases
TCCCTTCTTATCGTGCCGGAATACATCCCATATTTCGGCACGCTCACCGGATGCGGCATCAGTGCATTATCCGGATAACA
ATATTCTCCTATCCAACTTT

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase; Copper homeostasis protein CutE

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIA
TFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQPEKTIQVSMVQGDIPQSLKWDEG
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLG
KGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNF
RPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK

Sequences:

>Translated_512_residues
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIA
TFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQPEKTIQVSMVQGDIPQSLKWDEG
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLG
KGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNF
RPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
>Mature_511_residues
AFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIAT
FGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLK
GLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQPEKTIQVSMVQGDIPQSLKWDEGQ
LLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGK
GAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNFR
PDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGLT
PYARTGNWPLWVLTALFGFAAVLMSLRQRRK

Specific function: Transfers the fatty acyl group on membrane lipoproteins

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Escherichia coli, GI1786878, Length=512, Percent_Identity=99.609375, Blast_Score=1030, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LNT_ECO57 (Q8XBK2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85566
- PIR:   G90715
- RefSeq:   NP_286382.1
- RefSeq:   NP_308722.1
- ProteinModelPortal:   Q8XBK2
- EnsemblBacteria:   EBESCT00000026265
- EnsemblBacteria:   EBESCT00000055936
- GeneID:   917056
- GeneID:   957716
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0806
- KEGG:   ecs:ECs0695
- GeneTree:   EBGT00050000010323
- HOGENOM:   HBG616234
- OMA:   FESYFPE
- ProtClustDB:   PRK00302
- BioCyc:   ECOL83334:ECS0695-MONOMER
- HAMAP:   MF_01148
- InterPro:   IPR004563
- InterPro:   IPR003010
- Gene3D:   G3DSA:3.60.110.10
- TIGRFAMs:   TIGR00546

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 2.3.1.- [C]

Molecular weight: Translated: 57095; Mature: 56963

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x251a9bc8)-; HASH(0x24ce7fa0)-; HASH(0x40638818)-; HASH(0x25a7567c)-; HASH(0x25733cf8)-; HASH(0x25de63a0)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLKGLAPLMGVEAINFLLMMVS
HEEHHHHCHHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQPEKTIQVSMVQGDIPQSLKWDEG
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIV
HHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHH
DARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDL
HHHHCCCCCCCCCHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSI
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
AFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLKGLAPLMGVEAINFLLMMVS
HEEHHHHCHHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLLALALVKRNWRPLVVAVVLFALPFPLRYIRWFTPQPEKTIQVSMVQGDIPQSLKWDEG
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIV
HHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHH
DARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDL
HHHHCCCCCCCCCHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PMSSFSRGPYIQPPLSANGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSI
CHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796