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The map label for this gene is leuA [H]

Identifier: 209398496

GI number: 209398496

Start: 86546

End: 88117

Strand: Reverse

Name: leuA [H]

Synonym: ECH74115_0081

Alternate gene names: 209398496

Gene position: 88117-86546 (Counterclockwise)

Preceding gene: 209396438

Following gene: 209399607

Centisome position: 1.58

GC content: 53.12

Gene sequence:

>1572_bases
ATGAGCCAGCAAGTCATTATTTTCGATACCACATTGCGCGACGGTGAACAGGCGTTACAGGCAAGCTTGAGTGTGAAAGA
AAAACTGCAAATTGCGCTGGCCCTTGAGCGTATGGGTGTTGACGTGATGGAAGTCGGGTTCCCCGTCTCTTCGCCGGGTG
ATTTTGAATCAGTGCAAACCATCGCTCGCCAGGTTAAAAACAGCCGTGTATGCGCGTTAGCTCGCTGCGTGGAGAAAGAT
ATCGACGTGGCGGCCGAATCCCTGAAAGTCGCCGAAGCCTTCCGTATTCATACCTTTATTGCCACTTCGCCAATGCACAT
CGCCACCAAGCTGCGCAGCACGCTGGACGAGGTGATCGAACGCGCTATCTATATGGTGAAACGCGCCCGTAATTACACCG
ATGATGTTGAATTTTCTTGCGAAGATGCCGGGCGTACACCCATTGCCGATCTGGCGCGAGTGGTCGAAGCGGCGATTAAT
GCCGGTGCCACCACCATCAACATTCCGGACACCGTGGGCTACACCATGCCGTTTGAGTTCGCCGGAATCATTAGCGGCCT
GTATGAACGCGTGCCTAACATCGACAAAGCCATTATTTCCGTACATACCCACGACGATTTGGGCCTGGCGGTCGGCAACT
CACTGGCGGCGGTACATGCCGGTGCACGCCAGGTGGAAGGTGCAATGAACGGGATCGGCGAGCGTGCCGGTAACTGTTCC
CTGGAAGAAGTCATCATGGCGATTAAAGTTCGTAAGGATATTCTCAACGTCCACACCGCCATTAATCACCAGGAGATATG
GCGCACCAGCCAGTTAGTTAGCCAGATTTGTAATATGCCGATCCCGGCAAACAAAGCCATTGTTGGCAGCGGCGCATTCG
CACACTCCTCCGGTATCCACCAGGATGGCGTGCTGAAAAACCGCGAAAACTACGAAATCATGACACCAGAATCTATTGGT
CTGAACCAAATCCAGCTGAATCTGACCTCTCGTTCGGGGCGTGCGGCGGTGAAACATCGCATGGATGAGATGGGGTATAA
AGAAAGTGAATATAATTTAGACAATTTGTACGACGCCTTCCTCAAGCTGGCGGACAAAAAAGGTCAGGTGTTTGATTACG
ATCTGGAGGCGCTGGCCTTCATCGGTAAGCAGCAAGAAGAGCCGGAGCATTTCCGTCTGGATTACTTCAGCGTGCAGTCT
GGCTCTAACGATATCGCCACCGCCGCCGTCAAACTGGCCTGCGGCGAAGAAGTCAAAGCAGAAGCCGCCAACGGTAACGG
TCCGGTCGATGCCGTCTATCAGGCGATTAACCGCATCACTGACTATAACGTCGAACTGGTGAAATACAGCCTGACCGCCA
AAGGTCACGGTAAAGATGCGCTGGGTCAGGTGGATATTGTCGCCAACTACAACGGTCGCCGCTTCCACGGCGTCGGCCTG
GCCACCGATATTGTCGAGTCCTCCGCCAAAGCCATGGTGCACGTACTTAACAATATCTGGCGTGCCACAGAAGTCGAAAA
AGAGTTGCAACGCAAAGCTCAACACAACGAAAACAACAAGGAAACCGTGTGA

Upstream 100 bases:

>100_bases
AGCATTAAGCCAGCACGCAGTCAAACAAAAAACCCGCGCCATTGCGCGGGTTTTTTTATGCCCGAAGCGAGGCGCTCTAA
AAGAGACAAGGACCCAAACC

Downstream 100 bases:

>100_bases
TGTCGAAGAATTACCATATTGCCGTATTGCCGGGGGATGGCATTGGTCCGGAAGTGATGACCCAGGCGCTGAAAGTGCTG
GATGCCGTGCGCAACCGCTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKD
IDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQS
GSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRATEVEKELQRKAQHNENNKETV

Sequences:

>Translated_523_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKD
IDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQS
GSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRATEVEKELQRKAQHNENNKETV
>Mature_522_residues
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDI
DVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINA
GATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCSL
EEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGL
NQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSG
SNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGLA
TDIVESSAKAMVHVLNNIWRATEVEKELQRKAQHNENNKETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=523, Percent_Identity=99.6175908221797, Blast_Score=1077, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=27.5766016713092, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=27.2980501392758, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=24.8161764705882, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=24.2144177449168, Blast_Score=120, Evalue=7e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57315; Mature: 57183

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA
HHHHCCCCCEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHC
EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRATEVEKELQRKAQHNENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA
HHHHCCCCCEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHC
EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRATEVEKELQRKAQHNENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA