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The map label for this gene is hycA

Identifier: 209398474

GI number: 209398474

Start: 3678103

End: 3678564

Strand: Reverse

Name: hycA

Synonym: ECH74115_3976

Alternate gene names: 209398474

Gene position: 3678564-3678103 (Counterclockwise)

Preceding gene: 209397553

Following gene: 209399068

Centisome position: 66.02

GC content: 50.0

Gene sequence:

>462_bases
ATGACTATTTGGGAAATAAGCGAGAAAGCCGATTACATCGCACAGCGGCATCGTCGCCTACAGGACCAGTGGCACATCTA
CTGCAATTCGCTGGTTCAGGGGATCACGTTATCGAAAGCGCGCCTGCATCACGCCATGAGCTGCGCGCCGGACAAAGAAC
TCTGTTTCGTCCTTTTTGAACATTTTCGTATTTACGTCACCCTGGCGGATGGCTTTAACAGCCACACCATCGAGTATTAC
GTCGAAACAAAAGATGGCGAAGACAAACAGCGGATTGCGCAGGCGCAACTGAGCATTGACGGCATGATTGATGGCAAGGT
CAACATCCGCGATCGCGAACAGGTTCTGGAACACTATCTCGAAAAAATCGCTGGCGTTTACGACAGCTTATACACCGCTA
TTGAAAACAATGTGCCGGTGAATTTAAGCCAACTGGTAAAGGGACAAAGCCCGGCAGCATGA

Upstream 100 bases:

>100_bases
AATTCACCAAAATCTTAAAAATCGCTTTAATAATAATAAATTAAAAGTTGGCACAAAAAATGCTTAAAGCTGGCATCTCT
GTTAAACGGGTAACCTGACA

Downstream 100 bases:

>100_bases
GCTGAGGCTTTGCCCGTTTTGCAGGCGTTACGCCTGTTTGGGGATGGGCGTGTCGATGAGTGTCGAAAATGACATTTCAT
CGGCATGTTTTCGTCAAAAA

Product: formate hydrogenlyase regulatory protein HycA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYY
VETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA

Sequences:

>Translated_153_residues
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYY
VETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
>Mature_152_residues
TIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYV
ETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA

Specific function: Regulatory protein for the formate hydrogenlyase system. Could act by directly interacting with fhlA or by preventing the binding of fhlA to the upstream activatory sequence

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789080, Length=153, Percent_Identity=100, Blast_Score=322, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCA_ECO57 (P0AEV5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85921
- PIR:   E91076
- RefSeq:   NP_289274.1
- RefSeq:   NP_311608.1
- EnsemblBacteria:   EBESCT00000023877
- EnsemblBacteria:   EBESCT00000060320
- GeneID:   914697
- GeneID:   958172
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4033
- KEGG:   ecs:ECs3581
- GeneTree:   EBGT00050000009196
- HOGENOM:   HBG416926
- OMA:   HYLAIIA
- ProtClustDB:   PRK10198
- BioCyc:   ECOL83334:ECS3581-MONOMER
- GO:   GO:0006350
- InterPro:   IPR021285

Pfam domain/function: PF11046 HycA_repressor

EC number: NA

Molecular weight: Translated: 17627; Mature: 17496

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYL
HHEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEECCEECCCCHHHHHHHHH
EKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFE
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
HFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYL
HHEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCEEECCEECCCCHHHHHHHHH
EKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796