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The map label for this gene is rusA [H]

Identifier: 209398314

GI number: 209398314

Start: 2921210

End: 2921584

Strand: Reverse

Name: rusA [H]

Synonym: ECH74115_3154

Alternate gene names: 209398314

Gene position: 2921584-2921210 (Counterclockwise)

Preceding gene: 209397571

Following gene: 209397668

Centisome position: 52.43

GC content: 51.2

Gene sequence:

>375_bases
ATGCTCATAGATTTGGTTTTACCTTACCCGCCGACGGTGAACACTTACTGGCGACGCCGTGGCAGCACATATTTTGTATC
AAAAGCCGGGAAGCGTTATCGCCGGGCAGTGGCGCTTATTGTTCGCCAGCAGCGACTGAAATTAAGCCTGTCCGGACGGC
TGGCAATAAAAATTATTGCAGAGCCACCGGATAAGCGCCGCCGCGACCTGGACAATATCCTGAAAGCACCGCTGGATGCG
CTGACGCACGCGGGGTTGTTAATGGACGATGAGCAGTTTGATGAAATCAATATTGTACGTGGAAAGCCAGTATCTGGTGG
ACGTCTGGGGGTGAAGATTTACCCCATAATGCTTGAAGGGCAGGTCAAAAAATGA

Upstream 100 bases:

>100_bases
GCTGGCGTTCGAAGAAAAGCATGGTTCTCAGGTTGATTTAATTTTTCGTTTTCTTGATCACGCCTTTGCAACCGGCGTGC
TCGGGTAAAAGAGGTGACTG

Downstream 100 bases:

>100_bases
AACTGGAAGATTTACCGAAATACTACTCCCCAAAATCCCCTGGCCTGACCGATGCATCGGCCTCAACGTCAAAAGATGCG
CTGAGTATCACTGATGTGAT

Product: crossover junction endodeoxyribonuclease RusA

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEGQVKK

Sequences:

>Translated_124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEGQVKK
>Mature_124_residues
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDA
LTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEGQVKK

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=109, Percent_Identity=41.2844036697248, Blast_Score=86, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 14139; Mature: 14139

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQRLKLSLSGRLAIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEG
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCEEEEEEEEEEEEC
QVKK
EECC
>Mature Secondary Structure
MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQRLKLSLSGRLAIKIIA
CEEEEEECCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEEE
EPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEG
CCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCCEEEEEEEEEEEEC
QVKK
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA