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The map label for this gene is ygfT [H]

Identifier: 209398308

GI number: 209398308

Start: 3871559

End: 3873484

Strand: Reverse

Name: ygfT [H]

Synonym: ECH74115_4176

Alternate gene names: 209398308

Gene position: 3873484-3871559 (Counterclockwise)

Preceding gene: 209398148

Following gene: 209399415

Centisome position: 69.52

GC content: 52.75

Gene sequence:

>1926_bases
ATGCAAATGAATAAGTTTATCGCTGCTGAAGCCGCGGAATGTATAGGCTGTCATGCTTGTGAAATTGCCTGTGCGGTGGC
ACACAATCAAGAAAACTGGCCGCTGAGTCACAGTGACTTTCGACCACGTATCCACGTTGTAGGGAAAGGCCTGGCTGCGA
ATCCGGTGGCCTGCCATCACTGCAACAATGCCCCTTGCGTTACGGCTTGTCCGGTTAATGCTCTGACTTTCCAGTCCGAT
AGCGTACAACTGGACGAGCAAAAATGTATTGGTTGTAAAAGATGCGCAATCGCTTGCCCCTTTGGCGTCGTTGAGATGGT
CGATACGATTGCGCAGAAATGCGACCTTTGTAACCAGCGCAGTTCCGGCACGCAAGCCTGTATTGAAGTTTGCCCAACAC
AGGCGTTACGACTGATGGACGATAAAGGGTTACAGCAGATAAAGGTGGCCCGCCAGCGCAAAACGGCAGCAGGAAAAGCG
TCATCAGACGCTCAGCCATCTCGCAGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAGATAAAATTTCAGCAAG
TGAACGGAAAAACCACTTTGGCGAAATCTATTGCGGGCTGGATCCACAACAAGCGACTTATGAGAGTGACCGCTGTGTTT
ATTGTGCCGAAAAAGCTAACTGCAACTGGCATTGCCCGCTGCATAACGCTATTCCGGATTACATCCGTCTGGTACAGGAG
GGAAAGATTATTGAAGCGGCAGAACTTTGCCACCAGACCAGTTCCTTACCAGAAATCTGCGGCAGGGTATGTCCACAGGA
CCGTCTTTGTGAAGGCGCATGTACTTTGAAAGATCACTCTGGCGCAGTCTCTATCGGTAATCTGGAACGCTACATCACCG
ATACCGCGCTGGCGATGGGCTGGCGTCCCGATGTCAGCAAAGTTGTTCCCCGTATCGAGAAAGTGGCGGTGATTGGCGCT
GGGCCTGCAGGGTTAGGGTGTGCTGATATTCTGGCGCGCGCAGGAGTTCAGGTCGATGTCTTTGATCGCCATCCAGAAAT
TGGCGGTATGCTGACTTTTGGCATTCCTCCTTTCAAACTCGATAAAACGGTATTAAGCCAGCGGCGAGAGATATTCACCG
CAATGGGAATCGACTTTCATCTTAACTGTGAAATTGGCCGCGATATTACCTTTAGCGATTTAACTTCTGAATATGATGCA
GTTTTCATCGGCGTGGGGACTTACGGGATGATGCGAGCAGATCTGCCGCATGAAGATGCGCCCGGTGTCATTCAGGCTCT
ACCGTTCCTGACTGCCCATACCCGCCAGCTCATGGGATTGCCGGAGTCTGAAGAGTATCCGCTGACGGACGTGGAAGGTA
AGCGAGTCGTGGTATTGGGCGGTGGCGATACGACAATGGATTGTTTGCGGACTTCCATTCGCCTCAATGCTGCCAGCGTG
ACCTGCGCGTATCGTCGTGATGAAGTCAGTATGCCGGGCTCGCGCAAAGAGGTGGTCAATGCGCGCGAGGAAGGTGTCGA
GTTTCAATTCAATGTTCAGCCGCAATATATCGCTTGTGACGAAGATGGGCGTTTAACTGCGGTGGGCCTGATTCGTACCG
CCATGGGTGAGCCGGGGCCGGATGGTCGCCGTCGTCCTCGTCCGGTTGCGGGTTCAGAGTTTGAATTGCCCGCCGATGTT
CTCATTATGGCCTTTGGTTTCCAGGCGCATGCCATGCCGTGGTTGCAGGGCAGCGGAATTAAACTCGATAAATGGGGCCT
GATTCAAACCGGTGACGTCGGGTATTTACCTACCCAGACGCATCTGAAAAAAGTCTTTGCTGGTGGTGATGCAGTTCATG
GCGCGGATCTGGTTGTCACTGCAATGGCCGCAGGAAGGCAGGCGGCGCGCGATATGTTAACTCTGTTTGATACGAAGGCA
TCGTGA

Upstream 100 bases:

>100_bases
CTCAGTCGCAAAATTATAATTAAAACGGTCATGCTACCTAATATATGCCCGTTTATTGCATCGCGAAATTTGAGAAGACA
TAACTCATTAAATGAAAGGG

Downstream 100 bases:

>100_bases
TGAAATCGTTAATTATCGTTAATCCGGCTGACTGTATTGGCTGCCGTACTTGTGAAGTGGCCTGTGTGGTCGCTCACCCT
TCATAACAGGAGTTGAATGC

Product: putative oxidoreductase Fe-S binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MQMNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGLAANPVACHHCNNAPCVTACPVNALTFQSD
SVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKA
SSDAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQE
GKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGA
GPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDA
VFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASV
TCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADV
LIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKA
S

Sequences:

>Translated_641_residues
MQMNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGLAANPVACHHCNNAPCVTACPVNALTFQSD
SVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKA
SSDAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQE
GKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGA
GPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDA
VFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASV
TCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADV
LIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKA
S
>Mature_641_residues
MQMNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGLAANPVACHHCNNAPCVTACPVNALTFQSD
SVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKA
SSDAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQE
GKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGA
GPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDA
VFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASV
TCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADV
LIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKA
S

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=472, Percent_Identity=29.6610169491525, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI111118983, Length=271, Percent_Identity=25.830258302583, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI111118981, Length=162, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=639, Percent_Identity=99.5305164319249, Blast_Score=1316, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=656, Percent_Identity=54.2682926829268, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1789606, Length=450, Percent_Identity=59.5555555555556, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1788468, Length=449, Percent_Identity=28.9532293986637, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1789243, Length=475, Percent_Identity=32, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=44.8717948717949, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=46.0122699386503, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=39.3063583815029, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789079, Length=189, Percent_Identity=34.3915343915344, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87082114, Length=203, Percent_Identity=33.4975369458128, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI2367345, Length=167, Percent_Identity=32.3353293413174, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787122, Length=151, Percent_Identity=29.8013245033113, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787872, Length=159, Percent_Identity=30.188679245283, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1789370, Length=192, Percent_Identity=28.6458333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787963, Length=156, Percent_Identity=27.5641025641026, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1787749, Length=179, Percent_Identity=27.9329608938547, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=470, Percent_Identity=30.8510638297872, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71984108, Length=521, Percent_Identity=29.7504798464491, Blast_Score=158, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=254, Percent_Identity=28.740157480315, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=32.1041214750542, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=33.1958762886598, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=33.1958762886598, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=33.2644628099174, Blast_Score=220, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=33.2644628099174, Blast_Score=220, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24640763, Length=467, Percent_Identity=28.051391862955, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18858217, Length=467, Percent_Identity=28.051391862955, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00037 Fer4; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 69373; Mature: 69373

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGLAANPVACHH
CCCCHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEC
CNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQR
CCCCCEEEECCCCEEEECCCCCEECHHHCCCHHHHHEECCHHHHHHHHHHHHHHHHHCCC
SSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNSRKG
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
ADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQE
CCCCCHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHC
GKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMG
CCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHCC
WRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKL
CCCCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHH
DKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPHEDA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCC
PGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECEEE
TCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGP
EEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCCCCC
DGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQT
CCCCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCCCHH
HLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQMNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHVVGKGLAANPVACHH
CCCCHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEC
CNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQR
CCCCCEEEECCCCEEEECCCCCEECHHHCCCHHHHHEECCHHHHHHHHHHHHHHHHHCCC
SSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNSRKG
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
ADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQE
CCCCCHHHHHCCCCEEEECCCCCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHC
GKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTALAMG
CCEEHHHHHHHHCCCHHHHHHHHCCHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHCC
WRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGIPPFKL
CCCCHHHHHCHHHEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCHH
DKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRADLPHEDA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCC
PGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRTSIRLNAASV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECEEE
TCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVGLIRTAMGEPGP
EEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCEEEECCCCCEEHHHHHHHHCCCCCC
DGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQT
CCCCCCCCCCCCCCCCCHHHEEEEECCHHHCCCCCCCCCEEEECCCCEEECCCCCCCCHH
HLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]