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The map label for this gene is thiP [H]

Identifier: 209398294

GI number: 209398294

Start: 77711

End: 79321

Strand: Reverse

Name: thiP [H]

Synonym: ECH74115_0072

Alternate gene names: 209398294

Gene position: 79321-77711 (Counterclockwise)

Preceding gene: 209399031

Following gene: 209399712

Centisome position: 1.42

GC content: 58.97

Gene sequence:

>1611_bases
ATGGCAACGCGCCGTCAGCCGTTAATTCCCGGCTGGTTAATTCCAGGTGTAAGCGCCGCCACGCTGGTGGTAGCGGTTGC
GCTGGCGGCGTTTCTCGCCCTGTGGTGGAACGCGCCGCAGGGTGACTGGTCGGCAGTCTGGCAGGACAGCTACCTGTGGC
ATGTGGTGCGCTTCTCCTTCTGGCAGGCGTTTCTCTCGGCGCTACTCTCTGTCGTACCCGCGATATTCCTCGCCCGCGCG
CTCTATCGCAGGCGCTTTCCGGGTCGGCTGGCGCTGTTGCGTCTGTGCGCAATGACCTTGATCCTCCCGGTGCTGGTTGC
TGTTTTCGGCATTCTTAGCGTCTATGGTCGCCAGGGCTGGCTGGCATCGCTCTGCCAATCGCTCGGTCTGGAGTGGACCT
TTTCGCCCTACGGCCTGCAAGGCATTTTGCTGGCCCATGTGTTTTTTAATCTGCCGATGGCGAGCCGCTTATTACTCCAG
GCACTGGAAAACATTCCCGGCGAACAACGTCAGCTTGCCGCCCAGCTTGGGATGCGCGGCTGGCATTTTTTCCGCTTCGT
CGAATGGCCGTGGTTACGGCGACAAATCCCGCCGGTTACCGCGCTTATCTTTATGCTCTGTTTCGCCAGCTTCGCCACCG
TGTTATCGCTGGGCGGCGGTCCGCAGGCAACCACTATCGAGCTGGCAATCTATCAGGCGCTGAGTTACGACTACGATCCT
GCCCGCGCGGCGATGCTGGCGCTGATCCAGATGGTGTGTTGCCTTGGGCTGGTGCTGTTGAGTCAGCGACTGAGTAAGGC
CATTGCGCCCGGCACCACGCTGCTGCAAGGCTGGCGCGACCCGGACGATCGTCTGCATAGCCGCATTTGCGACACGATGT
TAATTGTGCTGGCGCTGCTGCTGTTGCTGCCACCGTTGCTGGCGGTGATCGTCGATGGGGTAAATCGCCAGTTGCCGGAA
GTGCTGGCACAACCGGTGCTGTGGCAGGCGCTGTGGACCTCGTTGCGTATTGCGCTGGCGGCAGGTGTATTGTGCGTAGT
GCTGACCATGATGCTGCTATGGAGCAGTCGCGAACTGCGGGCGCGGCAAAAAATGCTGGCGGGCCAGGCGCTGGAGATGA
GCGGCATGTTGATCCTCGCTATGCCGGGGATTGTGCTGGCAACGGGCTTCTTTTTACTGCTCAACAACACTATCGGCCTG
CCACAATCTGCTGACGGCATTGTGATTTTCACCAATGCGTTAATGGCGATCCCCTACGCCCTGAAAGTGCTGGAAAACCC
GATGCGCGATATCACCGCCCGCTACAGCATGTTATGTCAGTCGCTGGGGATTGAAGGCTGGTCACGCTTAAAAGTAGTGG
AGCTGCGCGCCCTGAAACGCCCGCTGGCGCAGGCGCTGGCCTTTGCCTGCGTGCTGTCGATTGGTGATTTTGGCGTGGTG
GCGTTGTTCGGTAACGATGATTTCCGCACCCTGCCGTTTTATCTCTACCAGCAAATTGGCTCCTATCGCAGCCAGGACGG
CGCGGTCACCGCGTTAATTCTGCTGCTGCTCTGTTTTCTGCTGTTTAGCGTGATTGAAAAAATACCGGGGCGAAATGTTA
AAACTGACTGA

Upstream 100 bases:

>100_bases
TCACGCTGCCTGCCGGTTTTGAAAAATTGACCAAACCCGCAACCACGCTGGAGTTCACGCCAGCCGAAGTGGCGGCACAA
CGTCAGGCATGGATTAGCGA

Downstream 100 bases:

>100_bases
TATCACCTGGCTTTATCACCATTTGCCGATGCGTTTTAGCTTAACGGTAGAACGCGGCGAGCAGGTGGCGATCCTCGGGC
CAAGCGGCGCGGGTAAAAGT

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWSAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARA
LYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
ARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPE
VLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGL
PQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFSVIEKIPGRNVKTD

Sequences:

>Translated_536_residues
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWSAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARA
LYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
ARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPE
VLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGL
PQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFSVIEKIPGRNVKTD
>Mature_535_residues
ATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWSAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARAL
YRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQA
LENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDPA
RAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTMLIVLALLLLLPPLLAVIVDGVNRQLPEV
LAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLP
QSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVVA
LFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFSVIEKIPGRNVKTD

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=536, Percent_Identity=97.7611940298508, Blast_Score=957, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59396; Mature: 59265

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWSAVWQDSYLWHVVRFSF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
WQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRG
HHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCH
WHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCH
ARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTMLIVLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLPQSADGIVIFTNALMAIPYA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHH
LKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFSVIEKIPGRNVKTD
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWSAVWQDSYLWHVVRFSF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
WQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRG
HHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCH
WHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCH
ARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTMLIVLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLPQSADGIVIFTNALMAIPYA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHH
LKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFSVIEKIPGRNVKTD
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]