Click here to switch to the map view.

The map label for this gene is aroF [H]

Identifier: 209398239

GI number: 209398239

Start: 3564308

End: 3565378

Strand: Reverse

Name: aroF [H]

Synonym: ECH74115_3840

Alternate gene names: 209398239

Gene position: 3565378-3564308 (Counterclockwise)

Preceding gene: 209398937

Following gene: 209399005

Centisome position: 63.99

GC content: 52.01

Gene sequence:

>1071_bases
ATGCAAAAAGACGCGCTGAATAACGTACATATTACCGACGAACAGGTTTTAATGACTCCGGCACAACTGAAGGCCGCTTT
TCCATTAAGCCTGCAACAAGAAGCCCAGATTGCTGACTCGCGTAAAACCATTTCAGATATTATCGCCGGGCGCGATCCTC
GTCTGCTGGTAGTATGTGGCCCTTGTTCCATTCATGATCCGGAAACTGCTCTGGAATATGCTCGTCGATTTAAAGCCCTT
GCCGCAGAGGTCAGCGATAGCCTCTATCTGGTAATGCGCGTCTATTTTGAAAAACCCCGTACCACTGTCGGCTGGAAAGG
GTTAATTAACGATCCCCATATGGATGGCTCTTTTGATGTAGAAGCCGGGCTGCAGATCGCGCGTAAATTGCTGCTTGAGC
TGGTGAATATGGGACTGCCACTGGCGACGGAAGCGTTAGATCCGAATAGCCCGCAATACCTGGGCGATCTGTTTAGCTGG
TCAGCAATTGGTGCTCGTACAACGGAATCGCAAACTCACCGTGAAATGGCCTCCGGGCTTTCCATGCCGGTTGGCTTTAA
AAACGGCACCGACGGCAGTCTGGCAACAGCAATTAACGCTATGCGCGCCGCCGCCCAGCCGCACCGTTTTGTTGGCATTA
ACCAGGCAGGGCAGGTTGCGTTGCTACAAACTCAGGGGAATCCGGACGGGCATGTCATCCTGCGCGGTGGTAAAGCGCCG
AACTATAGCCCTGCGGATGTTGCGCAATGTGAAAAAGAGATGGAACAGGCGGGACTGCGCCCGTCTCTGATGGTAGATTG
CAGCCACGGTAATTCCAATAAAGATTATCGCCGTCAGCCTGCGGTGGCAGAATCCGTGGTTGCTCAAATCAAAGATGGCA
ATCGCTCAATTATTGGTCTGATGATCGAAAGTAATATCCACGAGGGCAATCAGTCTTCCGAGCAACCGCGCAGTGAAATG
AAATACGGTGTATCCGTAACCGATGCCTGCATTAGCTGGGAAATGACCGATGCCTTGCTGCGTGAAATTCATCAGGATCT
GAACGGGCAGCTGACGGCTCGCGTGGCTTAA

Upstream 100 bases:

>100_bases
TGTACGAAATATGGATTGAAAACTTTACTTTATGTGTTATCGTTACGTCATCCTCGCTGAGGATCAACTATCGCAAACGA
GCATAAACAGGATCGCCATC

Downstream 100 bases:

>100_bases
GAGGTTTATTATGGTTGCTGAATTGACCGCATTACGCGATCAAATTGATGAAGTCGATAAAGCGCTGCTGAATTTATTAG
CGAAGCGTCTGGAACTGGTT

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA

Sequences:

>Translated_356_residues
MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
>Mature_356_residues
MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=356, Percent_Identity=99.438202247191, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1786969, Length=337, Percent_Identity=55.1928783382789, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787996, Length=329, Percent_Identity=49.8480243161094, Blast_Score=338, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6320240, Length=331, Percent_Identity=51.6616314199396, Blast_Score=356, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319726, Length=345, Percent_Identity=50.7246376811594, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38760; Mature: 38760

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]