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The map label for this gene is yfhA

Identifier: 209397901

GI number: 209397901

Start: 3511952

End: 3513286

Strand: Reverse

Name: yfhA

Synonym: ECH74115_3788

Alternate gene names: 209397901

Gene position: 3513286-3511952 (Counterclockwise)

Preceding gene: 209396789

Following gene: 209395723

Centisome position: 63.05

GC content: 54.23

Gene sequence:

>1335_bases
ATGAGCCATAAACCTGCGCATTTATTATTGGTCGATGACGATCCGGGATTGCTGAAACTGCTTGGCCTGCGCCTGACCAG
CGAAGGCTACAGCGTGGTCACGGCGGAAAGTGGCGCTGAAGGATTACGGGTACTGAATCGCGAAAAAGTAGATTTAGTCA
TCAGCGACCTGCGGATGGATGAAATGGACGGTATGCAGCTGTTTGCTGAAATCCAGAAAGTGCAGCCGGGAATGCCGGTA
ATTATTCTTACCGCGCATGGTTCTATTCCCGATGCCGTTGCTGCAACACAGCAGGGCGTTTTTAGTTTCCTCACCAAGCC
TGTTGATAAAGACGCGCTATATCAGGCAATTGATGATGCGCTGGAACAATCCGCGCCAGCCACCGACGAACGCTGGCGCG
AGGCGATTGTCACCCGCAGCCCGCTGATGCTGCGTTTGCTGGAACAGGCGCGGCTGGTGGCGCAATCAGACGTCAGCGTT
TTGATTAATGGCCAGAGCGGCACCGGGAAAGAGATTTTCGCCCAGGCTATCCACAACGCCAGCCCGCGCAATAGCAAACC
ATTTATTGCTATTAACTGTGGTGCATTGCCTGAGCAATTGCTGGAGTCGGAGCTGTTTGGTCATGCGCGTGGCGCGTTTA
CTGGCGCTGTCAGCAATCGCGAAGGTTTATTCCAGGCGGCGGAAGGCGGAACGCTATTTCTCGATGAGATTGGCGATATG
CCTGCGCCGTTGCAGGTCAAACTGTTGCGCGTGTTGCAGGAGCGTAAAGTGCGCCCGCTGGGCAGTAACCGCGATATTGA
TATCAATGTGCGGATTATTTCTGCCACTCACCGTGATCTGCCAAAAGCGATGGCGCGCGGGGAATTCCGCGAAGATCTCT
ATTACCGCCTCAACGTTGTCAGCCTGAAAATTCCGGCACTGGCGGAGCGCACAGAAGACATTCCGCTACTGGCAAATCAC
CTGTTGCGCCAGGCGGCAGAGCGACATAAACCGTTTGTCCGCGCGTTTTCCACCGATGCGATGAAACGCCTGATGACCGC
GAGCTGGCCGGGTAATGTGCGCCAGTTGGTCAACGTGATTGAACAGTGCGTGGCGCTGACCTCATCTCCGGTGATTAGCG
ATGCATTAGTAGAGCAGGCGCTGGAGGGTGAAAATACGGCGCTGCCAACCTTTGTTGAGGCACGTAATCAGTTTGAACTC
AACTATTTGCGTAAGCTGCTGCAAATTACCAAAGGCAACGTCACCCACGCGGCGAGAATGGCGGGGCGCAACCGGACAGA
ATTTTATAAACTGCTTTCCCGACACGAGCTGGATGCAAACGATTTCAAGGAATGA

Upstream 100 bases:

>100_bases
GAACGCCAGCTCTCGACCCGCAAACCGGCTGGAAATTTCTCGCCGGATACGCCACACGAGAGTGAAAAGCCCGCGCCATC
CACCCATGAGGTCACTCCTG

Downstream 100 bases:

>100_bases
ATTGGCGTTATGTGTTACGTTTAGCAGATCAAAAGACAGGCGACCTTTTCAAGGAATAGCATGAAAAAGATTGATGCGAT
TATTAAACCCTTCAAGCTGG

Product: sigma-54 dependent response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_443_residues
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVI
ILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVL
INGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDMP
APLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHL
LRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELN
YLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=100, Blast_Score=906, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=44.3946188340807, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.2197802197802, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=38.1465517241379, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=55.1724137931034, Blast_Score=264, Evalue=9e-72,
Organism=Escherichia coli, GI1789087, Length=307, Percent_Identity=46.5798045602606, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=48.4126984126984, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=39.873417721519, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1786524, Length=381, Percent_Identity=38.0577427821522, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=37.888198757764, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=42.6778242677824, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=36, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=33.1210191082803, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): YFHA_ECO57 (P0AFU5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91056
- PIR:   H85900
- RefSeq:   NP_289110.1
- RefSeq:   NP_311447.1
- ProteinModelPortal:   P0AFU5
- SMR:   P0AFU5
- PRIDE:   P0AFU5
- EnsemblBacteria:   EBESCT00000028789
- EnsemblBacteria:   EBESCT00000055590
- GeneID:   914905
- GeneID:   957883
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3830
- KEGG:   ecs:ECs3420
- GeneTree:   EBGT00050000008655
- HOGENOM:   HBG740884
- OMA:   SFSTDAM
- ProtClustDB:   PRK15115
- BioCyc:   ECOL83334:ECS3420-MONOMER
- GO:   GO:0005622
- GO:   GO:0006350
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 49149; Mature: 49018

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMD
CCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLPKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRVLNREKVDLVISDLRMD
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISATHRDLPKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796