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The map label for this gene is nadR [H]

Identifier: 209397888

GI number: 209397888

Start: 5557738

End: 5558970

Strand: Direct

Name: nadR [H]

Synonym: ECH74115_5904

Alternate gene names: 209397888

Gene position: 5557738-5558970 (Clockwise)

Preceding gene: 209397317

Following gene: 209395789

Centisome position: 99.74

GC content: 51.01

Gene sequence:

>1233_bases
ATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTAT
GACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTT
TTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACAT
ATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGC
GTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGCTGGTTATTGCAAACTTTTAAATATC
AGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGT
ATCAAAAAGTTTATGGCTGAAAAGGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATAT
GGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAA
ACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAG
TCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTA
TGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAAT
ACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAA
AAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAA
CAACACGCCGTGGGTGGTGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGTAAAGAGTTCCAGAATCTGCTGGTGG
AGATGCTGGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGCCGTTTCCTGCGCTGCGTGGAG
CTGGTGCGGGAGATGATGGGGGAGCAGAGATAA

Upstream 100 bases:

>100_bases
CGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAA
TGAGAAATACGGAGGGAGAT

Downstream 100 bases:

>100_bases
CCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGA
GATTAAACAAGGTTTGAAAA

Product: nicotinamide-nucleotide adenylyltransferase

Products: NA

Alternate protein names: Transcriptional regulator NadR; Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGH
IYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCK
KYEGREHPFVQALIDEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVE
LVREMMGEQR

Sequences:

>Translated_410_residues
MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGH
IYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCK
KYEGREHPFVQALIDEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVE
LVREMMGEQR
>Mature_409_residues
SSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHI
YLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGI
KKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGES
SGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKK
YEGREHPFVQALIDEYRFDLVILLENNTPWVVDGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVEL
VREMMGEQR

Specific function: This enzyme has three activities:DNA binding, nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The DNA-binding domain binds to the nadB operator sequence in an NAD- and ATP-dependent manner. As NAD levels increase

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=410, Percent_Identity=99.7560975609756, Blast_Score=857, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 47374; Mature: 47243

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50943 HTH_CROC1 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP
CCCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
RQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTV
CCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCCCCCCC
PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGIQPDLIYTSEE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
ADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE
CCCHHHHHHCCCEEEEECCHHHHHCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCC
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVK
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHH
YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVVDGLRSLG
HHCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
SSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVELVREMMGEQR
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP
CCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
RQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDDTRDRALFEDSAMSQQPTV
CCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHCCCCCCCCC
PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKKFMAEKGIQPDLIYTSEE
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCC
ADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRYWEYIPTEVKPFFVRTVAILGGE
CCCHHHHHHCCCEEEEECCHHHHHCCCCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCC
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEIALQYSDYDKIALGHAQYIDFAVK
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECHHHHHHHHHH
YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVVDGLRSLG
HHCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
SSVDRKEFQNLLVEMLEENNIEFVRVEEEDYDSRFLRCVELVREMMGEQR
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 1327967 [H]