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The map label for this gene is rfaY [H]

Identifier: 209397608

GI number: 209397608

Start: 4650338

End: 4650844

Strand: Reverse

Name: rfaY [H]

Synonym: ECH74115_4998

Alternate gene names: 209397608

Gene position: 4650844-4650338 (Counterclockwise)

Preceding gene: 209396863

Following gene: 209398034

Centisome position: 83.47

GC content: 30.97

Gene sequence:

>507_bases
ATGACCGAACGTTTCTTAAAATCTTGCATAAAAAAAGATTATTATGAAAATTTAATTTATCAAACCGATCGGGTACGTGG
TGAAGGCATCCAATCAATTAATGACTATTTTTTGCTAGCTGAACGTAAAACATTAAATTTCGCTCATTATTATATTATGC
TCATTGAGTATATTGAAGGTGTCGGGTTAAATGAGTATTTGGAAATTTCTGAAGATCTGAAGGATCAATTATCAGAATCG
ATAAAAGAGTTGCATCAGCATGGAATGGTGTCTGGCGATCCTCATAAGGGAAATTTTATAGTATCCGAAAAAGGTTTAAG
ACTAATTGACCTTTCAGGTAAAAAAACAACCGCTGTTTTAAAAGCAAAAGATCGCATTGATCTTGAACGGCACTATAATA
TAAAAAATGAATTGAAAGACTTTGGCTATACATATCTTATATTCAAAAAGAAAATTAAAAAAGTAATCAGGGATGTAAAA
GTCAAACTAGGATTGAAGAGCAAATAA

Upstream 100 bases:

>100_bases
AACATTAAAAGTTTTTCGTTCTATTGATGATACTAAAGTCATTCTTATTGACACTGCTCGTGGACCTCTTGTACTTAAAG
TTTATGCGCCAAAGCATAAA

Downstream 100 bases:

>100_bases
TGATTATAATGAAGGGTAATTAAATTGGATTTTAAACATCTTACTCAATTTAAAGATATAATTGAACTGGACAAGCGCCC
CGTTAAACTTGATGAACGGG

Product: lipopolysaccharide core biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSES
IKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVK
VKLGLKSK

Sequences:

>Translated_168_residues
MTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSES
IKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVK
VKLGLKSK
>Mature_167_residues
TERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESI
KELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKV
KLGLKSK

Specific function: Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protein kinase superfamily. RfaY/waaY family [H]

Homologues:

Organism=Escherichia coli, GI1790055, Length=161, Percent_Identity=57.1428571428571, Blast_Score=197, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR009330 [H]

Pfam domain/function: PF06176 WaaY [H]

EC number: 2.7.-.-

Molecular weight: Translated: 19727; Mature: 19596

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHC
VGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCEEHEE
KAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK
EHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHC
VGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEECCCCEEHEE
KAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK
EHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624462; 8041620; 9278503 [H]