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The map label for this gene is iclR

Identifier: 209397219

GI number: 209397219

Start: 5134318

End: 5135142

Strand: Reverse

Name: iclR

Synonym: ECH74115_5488

Alternate gene names: 209397219

Gene position: 5135142-5134318 (Counterclockwise)

Preceding gene: 209398575

Following gene: 209399314

Centisome position: 92.16

GC content: 54.42

Gene sequence:

>825_bases
ATGGTCGCACCCATTCCCGCGAAACGCGGCAGAAAACCCGCCGTTGCCACCGCACCAGCGACTGGACAGGTTCAGTCTTT
AACGCGTGGCCTGAAATTACTGGAGTGGATTGCCGAATCCAATGGCAGTGTGGCACTCACGGAGCTGGCGCAACAAGCCG
GGTTACCCAATTCCACGACCCACCGCCTGCTAACCACGATGCAACAGCAGGGTTTCGTGCGTCAGGTTGGCGAACTGGGA
CACTGGGCAATCGGCGCACATGCCTTTATGGTCGGCAGCAGCTTTCTCCAGAGCCGTAATTTGTTAGCAATTGTTCACCC
TATCCTGCGCAATCTAATGGAAGAGTCTGGCGAAACGGTCAATATGGCGGTGCTTGATCAAAGCGATCACGAAGCGATTA
TTATCGACCAGGTACAGTGTACGCATCTGATGCGAATGTCCGCGCCTATCGGCGGTAAATTGCCGATGCACGCTTCCGGT
GCGGGTAAAGCCTTTTTAGCCCAACTGAGCGAAGAACAGGTGACGAAGCTGCTGCACCGCAAAGGGTTACATGCCTATAC
CCACGCAACGCTGGTGTCTCCTGTGCATTTAAAAGAAGATCTCGCCCAAACGCGCAAACGGGGTTATTCATTTGACGATG
AGGAACATGCGCTGGGGCTACGTTGCCTTGCAGCGTGTATTTTCGATGAGCACCGTGAACCGTTTGCCGCAATTTCTATT
TCCGGACCGATTTCACGTATTACCGATGACCGCGTGACCGAGTTTGGCGCGATGGTGATTAAAGCGGCGAAGGAAGTGAC
GCTGGCGTACGGTGGAATGCGCTGA

Upstream 100 bases:

>100_bases
ATTTGTTCAACATTAACTCATCGGATCAGTTCAGCAACTATTGCATTAGCTAACAATAAAAATGAAAATGATTTCCACGA
TACAGAAAAAGGAGACTGTC

Downstream 100 bases:

>100_bases
CTTTTTCTGGCGGGCAGAGGCAATATTCTGCCCGTCATACCTGAGTGGTAATAGAATAAGGGTGTCTGTTAATCGCATTG
ACGTCAAAATAACTTATTGT

Product: transcriptional repressor IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR

Sequences:

>Translated_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
>Mature_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain

Homologues:

Organism=Escherichia coli, GI87082359, Length=274, Percent_Identity=100, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1786715, Length=264, Percent_Identity=42.4242424242424, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=29.8387096774194, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=30.241935483871, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789999, Length=227, Percent_Identity=33.4801762114537, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790752, Length=249, Percent_Identity=26.1044176706827, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=24.7011952191235, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICLR_ECOLI (P16528)

Other databases:

- EMBL:   M31761
- EMBL:   M63914
- EMBL:   U00006
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M63497
- RefSeq:   AP_004519.1
- RefSeq:   NP_418442.2
- PDB:   1TD5
- PDB:   2O99
- PDB:   2O9A
- PDBsum:   1TD5
- PDBsum:   2O99
- PDBsum:   2O9A
- ProteinModelPortal:   P16528
- SMR:   P16528
- DIP:   DIP-10008N
- IntAct:   P16528
- MINT:   MINT-1226737
- STRING:   P16528
- EnsemblBacteria:   EBESCT00000001328
- EnsemblBacteria:   EBESCT00000016989
- GeneID:   948524
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3978
- KEGG:   eco:b4018
- EchoBASE:   EB0486
- EcoGene:   EG10491
- eggNOG:   COG1414
- GeneTree:   EBGT00050000008790
- HOGENOM:   HBG739407
- OMA:   KMISTIQ
- ProtClustDB:   PRK11569
- BioCyc:   EcoCyc:PD04099
- BindingDB:   P16528
- Genevestigator:   P16528
- GO:   GO:0006350
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00346

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR

EC number: NA

Molecular weight: Translated: 29740; Mature: 29740

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS51077 HTH_ICLR; PS51078 ICLR_ED

Important sites: BINDING 212-212 BINDING 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429