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The map label for this gene is nuoJ

Identifier: 209397133

GI number: 209397133

Start: 3162874

End: 3163428

Strand: Reverse

Name: nuoJ

Synonym: ECH74115_3419

Alternate gene names: 209397133

Gene position: 3163428-3162874 (Counterclockwise)

Preceding gene: 209399174

Following gene: 209397713

Centisome position: 56.77

GC content: 52.97

Gene sequence:

>555_bases
ATGGAGTTCGCTTTTTATATCTGTGGCCTGATAGCCATACTTGCGACCTTGCGAGTGATCACCCATACCAATCCGGTACA
CGCACTGCTGTACCTGATTATTTCGCTGCTGGCGATTTCCGGGGTGTTCTTCTCACTAGGCGCTTACTTCGCCGGTGCGC
TGGAAATTATCGTCTACGCGGGTGCCATTATGGTGCTGTTCGTGTTCGTGGTGATGATGCTCAACCTGGGCGGTTCAGAA
ATCGAACAAGAACGCCAGTGGCTGAAACCGCAGGTGTGGATTGGTCCGGCAATTTTGTCAGCCATCATGTTGGTGGTGAT
TGTTTACGCCATTCTCGGTGTTAACGATCAAGGTATCGACGGTACGCCAATCAGTGCTAAAGCAGTGGGTATTACGCTGT
TCGGGCCTTACGTACTGGCGGTGGAACTGGCTTCTATGCTGCTGCTCGCGGGTCTGGTTGTGGCCTTCCACGTCGGTCGT
GAAGAGCGTGCGGGTGAAGTGCTGAGCAATCGTAAAGACGACAGCGCGAAAAGAAAAACGGAGGAGCACGCATGA

Upstream 100 bases:

>100_bases
GGATGGCAGGTATGGCAATCGACGGCAAAGATAAGGGCGAAGCAGAGAACGAAGCCAAGCCTATCGACGTCAAGAGCCTG
TTACCGTAAGGAGAGGGGCA

Downstream 100 bases:

>100_bases
TCCCCTTACAACATGGACTGATCCTCGCGGCAATCTTATTCGTTCTTGGCTTAACCGGTCTGGTTATCCGTCGCAATCTG
CTGTTTATGCTGATTGGTCT

Product: NADH dehydrogenase subunit J

Products: NA

Alternate protein names: NADH dehydrogenase I subunit J; NDH-1 subunit J; NUO10

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSE
IEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
EERAGEVLSNRKDDSAKRKTEEHA

Sequences:

>Translated_184_residues
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSE
IEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
EERAGEVLSNRKDDSAKRKTEEHA
>Mature_184_residues
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSE
IEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
EERAGEVLSNRKDDSAKRKTEEHA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0839

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 6 (chain J)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 6 family

Homologues:

Organism=Escherichia coli, GI1788616, Length=184, Percent_Identity=100, Blast_Score=365, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOJ_ECO57 (P0AFE2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91024
- PIR:   E85868
- RefSeq:   NP_288854.1
- RefSeq:   NP_311191.1
- ProteinModelPortal:   P0AFE2
- EnsemblBacteria:   EBESCT00000026499
- EnsemblBacteria:   EBESCT00000058876
- GeneID:   916872
- GeneID:   961418
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3539
- KEGG:   ecs:ECs3164
- GeneTree:   EBGT00050000011156
- HOGENOM:   HBG729722
- OMA:   ALFGPYV
- ProtClustDB:   PRK06638
- BioCyc:   ECOL83334:ECS3164-MONOMER
- InterPro:   IPR001457

Pfam domain/function: PF00499 Oxidored_q3

EC number: =1.6.99.5

Molecular weight: Translated: 19875; Mature: 19875

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xf986948)-; HASH(0x409e73fc)-; HASH(0x100b2d20)-; HASH(0x103636d4)-; HASH(0x409deb40)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
GTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGREERAGEVLSNRKDDSAKRKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
EEHA
HCCH
>Mature Secondary Structure
MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFFSLGAYFAGALEIIVYA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAIMLVVIVYAILGVNDQGID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
GTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGREERAGEVLSNRKDDSAKRKT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
EEHA
HCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796