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The map label for this gene is menE [H]

Identifier: 209397084

GI number: 209397084

Start: 3145523

End: 3146878

Strand: Reverse

Name: menE [H]

Synonym: ECH74115_3402

Alternate gene names: 209397084

Gene position: 3146878-3145523 (Counterclockwise)

Preceding gene: 209397997

Following gene: 209395927

Centisome position: 56.48

GC content: 56.19

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTTAATGACGAGCA
ACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTAGCCTCCGGATTTGCGGTGCAGGGTGTGGTTGAGGACAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTCGCTTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
TGGGGAAAACACGTTTCCGGCATTAACGTCGCTGCACATTCAGCGGGTTGAAGGCGCACATGTCGCTACGTGGCAGCCGA
CGCGTCTGTGCTCAATGACGTTGACCTCGGGTTCTACCGGGTTGCCGAAAGCCGCTGTCCATACTTATCAGGCCCATCTT
GCCAGTGCCGAAGGGGTGTTATCGCTGATTCCGTTTGGCGATCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTGCGTGATAAACAGCCATTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGCAGTTCCGTTTCCCTG
AAAGCGGTGTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTGTGTGCGAAAGAAGCCGACGGCCTGGCAGACGTTGGTTCGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATAATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACTGGTTTCACTGGTTAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTATTCTTCAGTGGCGGAGAGGGTATTCAGACGGAAGAAGTCGAGCGCGTAATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTCCCCGTTGCCGACAAGGAGTTTGGTCATCGACCGGTGGCGGTGATGGAGTAT
GACCACGAGAGCGTTGATCTTAGTGAATGGGTGAAAGATAAGCTGGCCCGTTTTCAGCAACCGGTACGTTGGCTGACTTT
GCCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAGTGGGTGCAACGTCAGCAATAA

Upstream 100 bases:

>100_bases
CAGGGCTGGACACGCTGGATCTAATGCAGGCGCAGCAGGTACGTCGCTGGCCGGGTAGCACGCTGCCTGTCGTGGAAGTT
GATGCACTGGAGCGGTTGTT

Downstream 100 bases:

>100_bases
TTAACCCTCCGTTTCACTTCTCAGTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATA
TGAAAACTCTTAGCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEY
DHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEY
DHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAVQGVVEDSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQTEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEY
DHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=98.669623059867, Blast_Score=908, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=378, Percent_Identity=25.1322751322751, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=23.030303030303, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=23.3201581027668, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788107, Length=294, Percent_Identity=27.2108843537415, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789201, Length=271, Percent_Identity=26.1992619926199, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=459, Percent_Identity=24.1830065359477, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=454, Percent_Identity=25.9911894273128, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=356, Percent_Identity=27.247191011236, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=356, Percent_Identity=27.247191011236, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=356, Percent_Identity=27.247191011236, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=24.4488977955912, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=363, Percent_Identity=25.068870523416, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25141359, Length=399, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=25.7627118644068, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538037, Length=357, Percent_Identity=23.8095238095238, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=22.5988700564972, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50289; Mature: 50289

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAVQGVVEDSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
QRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
HHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQTEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYDHESVDLSEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGFAVQGVVEDSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
QRVEGAHVATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
HHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCEEECCCC
GIQTEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYDHESVDLSEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]