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The map label for this gene is yfiE [H]

Identifier: 209397058

GI number: 209397058

Start: 3538753

End: 3539679

Strand: Reverse

Name: yfiE [H]

Synonym: ECH74115_3814

Alternate gene names: 209397058

Gene position: 3539679-3538753 (Counterclockwise)

Preceding gene: 209396160

Following gene: 209400058

Centisome position: 63.53

GC content: 48.65

Gene sequence:

>927_bases
ATGTTTTTACATTCTAAATTCGATATTTTCGATAGGTTTGGGGTTATGGATCTGCGCCGTTTTATTACGCTTAAAACCGT
GGTGGAAGAGGGTTCCTTTTTGCGAGCTTCGCAAAAATTGTGCTGTACACAATCGACGGTGACTTTTCATATTCAGCAGC
TTGAGCAGGAGTTCTCGGTACAGTTATTTGAGAAAATTGGCCGACGGATGTGCCTCACTCGCGAAGGTAAAAAGCTGCTG
CCGCACATTTATGAACTCACCAGAGTGATGGATACCCTTCGTGAGGCTGCCAAAAAGGAGTCGGATCCGGACGGGGAACT
ACGCGTTGTTTCGGGCGAAACGTTGCTCTCTTACCGAATGCCGCAGGTGTTGCAGCGTTTTCGGCAACGCGCCCCAAAAG
TGCGTTTATCGTTGCAGTCTCTGAACTGTTACGTGATCCGTGATGCGCTATTGAATGATGAGGCCGATGTCGGGGTTTTT
TATCGCGTAGGGAATGATGATGCCCTGAACCGACGAGAATTGGGTGAGCAATCGCTGGTGCTGGTAGCTTCACCGCAAAT
TGCAGATGTCGATTTTACTGAGCCGGGAAGACATAACGCCTGTAGCTTTATTATCAACGAACCGCAATGTGTATTCCGGC
AGATATTTGAGAGCACGCTGCGCCAGCGGCGGATCACGGTGGAAAACACCATTGAGCTGTTAAGCATTGAAAGTATCAAA
CGTTGTGTCGCGGCGAATATCGGTGTCAGTTACTTGCCCCGCTTTGCGGTGGAAAAAGAGCTTGAGAGCGGAGAATTAAT
TGAATTGCCTTTTGGCGAACACTCGCAGACCATTACGGCAATGTGCGCTCACCATGCCGGAAAAGCGGTTAGCCCGGCGA
TGCACACTTTTATTCAGTGTGTTGAAGATTGTTTTTTGCCGGGATAA

Upstream 100 bases:

>100_bases
TCAGGGTGTAAGTCCAAAAAGCACTTAAAAGGGTCGGTGTCACTTGTTATCTCCTCTGTTTTGGTAGACAGTACCCGACA
CAGAATATTTAAAAAAATGA

Downstream 100 bases:

>100_bases
AAATAATGCCGGGCTTAAACCCGGCATTGAGATTACTCTTCTGTCGTTTGCGGTGGCACGCCCGTTCCGCTTGGTTTACG
GCGCTTACCAATATTTTTGG

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFLHSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVSPAMHTFIQCVEDCFLPG

Sequences:

>Translated_308_residues
MFLHSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVSPAMHTFIQCVEDCFLPG
>Mature_308_residues
MFLHSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSVQLFEKIGRRMCLTREGKKLL
PHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVF
YRVGNDDALNRRELGEQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
RCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVSPAMHTFIQCVEDCFLPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=293, Percent_Identity=97.2696245733788, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.7425742574257, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787806, Length=287, Percent_Identity=24.0418118466899, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786401, Length=308, Percent_Identity=26.9480519480519, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=30.625, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=284, Percent_Identity=25.7042253521127, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=21.8106995884774, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=22.680412371134, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082024, Length=271, Percent_Identity=25.4612546125461, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788296, Length=137, Percent_Identity=34.3065693430657, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35178; Mature: 35178

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLHSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVSPAMHTFIQC
HHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
VEDCFLPG
HHHHCCCC
>Mature Secondary Structure
MFLHSKFDIFDRFGVMDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLSYRM
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHH
PQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLV
HHHHHHHHHHCCCEEEEHHHCCEEEEEHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCEE
LVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIK
EEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVSPAMHTFIQC
HHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
VEDCFLPG
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]