Click here to switch to the map view.

The map label for this gene is frvB [H]

Identifier: 209396987

GI number: 209396987

Start: 4992664

End: 4994109

Strand: Reverse

Name: frvB [H]

Synonym: ECH74115_5350

Alternate gene names: 209396987

Gene position: 4994109-4992664 (Counterclockwise)

Preceding gene: 209396615

Following gene: 209396256

Centisome position: 89.63

GC content: 55.53

Gene sequence:

>1446_bases
GTGGCGATGGAGTCATCCTTACGTATTGTCGCGATCACCAACTGCCCCGCCGGGATCGCTCACACCTACATGGTGGCGGA
AGCCCTGGAACAGAAAGCCCGTTCTCTCGGCCATACCATTAAAGTGGAAACTCAAGGGTCCAGCGGCGTTGAAAACCGCT
TATCCAGCGAAGAGATTGCCGCTGCCGATTACGTGATTCTCGCCACCGGGCGTGGCCTGAGCGGTGACGATCGCGCACGG
TTTGCCGGGAAAAAAGTTTATGAGATTGCCATCTCCCAGGCGCTGAAAAATATCGATCAGATTTTCAGTGAATTACCGAC
AAATTCGCAGCTTTTTGCCGCAGATAGCGGCGTGAAGCTAGGCAAACAGGAAGTACAAAGCGGCAGCGTGATGAGCCACC
TGATGGCGGGCGTCTCTGCGGCGCTGCCGTTTGTCATCGGCGGTGGTATCCTGGTGGCGCTTGCCAACATGCTGGTGCAG
TTCGGTTTGCCGTATACCGATATGTCGAAAGGCGCGCCGTCGTTTACCTGGGTAGTTGAATCCATCGGTTACCTTGGCTT
CACCTTTATGATCCCCATCATGGGCGCTTATATTGCCTCGTCGATTGCCGATAAACCGGCTTTCGCTCCGGCGTTTCTGG
TTTGCTATCTGGCGAACGACAAAGCGCTGCTCGGCACCCAGTCGGGTGCGGGATTCCTCGGCGCGGTGGTGCTGGGGCTG
GCGATTGGCTATTTCGTCTTCTGGTTCCGTAAAGTCCGTCTTGGCAAAGCATTACAACCGCTGCTCGGCTCCATGCTTAT
CCCATTCGTTACTCTGCTGGTTTTTGGCGTGCTCACTTACTACGTTATCGGGCCGGTGATGTCCGACCTCATGGGCGGGC
TACTGCACTTCCTGAATACCATTCCTCCATCAATGAAGTTTGCGGCAGCGTTTCTGGTCGGTGCGATGCTGGCGTTCGAT
ATGGGCGGCCCCATCAATAAAACCGCCTGGTTCTTCTGCTTCTCACTGCTGGAAAAACACATTTATGACTGGTACGCCAT
CGTCGGCGTTGTCCCCGTCGCGGCGGGTCTTGCGACGTTCATTGCGCCCAAACTGTTTACCCGACAGGAAAAAGAGGCTG
CCAGTAGTGCCATTGTGGTCGGTGCCACAGTCGCAACCGAACCGGCTATTCCTTACGCTCTGGCAGCTCCGTTGCCGATG
ATTACCGCCAATACGCTGGCGGGCGGCATTACCGGCGTGCTGGTGATCGCTTTCGGAATCAAACGTCTGGCACCTGGTTT
GGGTATCTTTGACCCGCTGATCGGCCTGATGTCGCCGGTAGGTTCGTTTTATCTGGTGCTGGCGATTGGCCTCGCGCTGA
ACATTTCATTCATTATCGTCCTGAAAGGATTGTGGTTACGCCGCAAAGCGAAAGCCGCGCAACAGGAGCTTGTCCATGAA
CATTGA

Upstream 100 bases:

>100_bases
TGTCGCAAAATTATTCACCAGGAATTTATTCATCAACTGCAACAGGGCGATACCGACCAGGTGCTTGCCTTGTTAAATCA
AACCCTCAGCTCATAAGGAA

Downstream 100 bases:

>100_bases
GTTACTGCAACAGTTGTGCGAAGCCAGCGCCGTCAGCGGCGATGAACAGGAAGTTCGCGACATTCTGATAAACACGCTGG
AACCTTGCGTTAATGAGATC

Product: putative PTS system transporter subunits IIBC

Products: NA

Alternate protein names: Fructose-like phosphotransferase enzyme IIB component; PTS system fructose-like EIIB component; Fructose-like permease IIC component; PTS system fructose-like EIIC component [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRAR
FAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQ
FGLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFD
MGGPINKTAWFFCFSLLEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPM
ITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
H

Sequences:

>Translated_481_residues
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRAR
FAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQ
FGLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFD
MGGPINKTAWFFCFSLLEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPM
ITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
H
>Mature_480_residues
AMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIAAADYVILATGRGLSGDDRARF
AGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQF
GLPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGLA
IGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDM
GGPINKTAWFFCFSLLEKHIYDWYAIVGVVPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMI
TANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHEH

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI87082348, Length=483, Percent_Identity=99.1718426501035, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1788492, Length=444, Percent_Identity=34.2342342342342, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1786951, Length=479, Percent_Identity=32.776617954071, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1790386, Length=291, Percent_Identity=35.7388316151203, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=50, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788729, Length=321, Percent_Identity=27.1028037383178, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788730, Length=100, Percent_Identity=40, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790390, Length=70, Percent_Identity=45.7142857142857, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 50911; Mature: 50780

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIA
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHH
AADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSELPTNSQLFAADSGVKL
HCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHH
GKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFGLPYTDMSKGAPSFTWVVE
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
SIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLANDKALLGTQSGAGFLGAVVLGL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCEEEECCCCCHHHHHHHHHH
AIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVPVAAGLATF
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAPKLFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGI
HHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KRLAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
H
C
>Mature Secondary Structure 
AMESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQGSSGVENRLSSEEIA
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCHHHHH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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503; 8019415 [H]