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The map label for this gene is rfaI [H]

Identifier: 209396863

GI number: 209396863

Start: 4651071

End: 4652078

Strand: Reverse

Name: rfaI [H]

Synonym: ECH74115_4999

Alternate gene names: 209396863

Gene position: 4652078-4651071 (Counterclockwise)

Preceding gene: 209396290

Following gene: 209397608

Centisome position: 83.49

GC content: 31.25

Gene sequence:

>1008_bases
ATGTCTCAACTCAATGATAGTGACATCATCCTTTTTGAGTATAATTTTCATTATCAAAATATAAGATCTAAAAATACTCT
TGATATAGCATTTGGTATTGACAGAAATTTTCTTTTTGGATGTGGTGTAGCCATCGCATCTATTCTATTAAACAATAGAG
AAATCTCTTGTGAATTTCATGTTTTCACAGATTATATCAGTGATAAAGACAAATTATATTTTTCTGATTTAGCAAAACAA
TATAATTCAAGAATTAATATTTATGTTATCAATTGTGATAAGCTGAAGTCATTACCAAGCACGAAAAACTGGACTTACGC
AACATATTTTCGATTTATAATTGCAGATTATTTCTATCATAAACATGAAAAAATACTATATCTTGATGCAGATATTGCTT
GCAAGGGTAGTATTAAAGAACTCTTAGATTATCAATTTTCTACTAATGAAATTGCCGCAGTTGTAGCTGAAAGAGATGTT
GAATGGTGGCAAAATCGAGCCTCGGTATTAACTACACCACAGTTAGCTTCTGGATATTTCAATGCTGGTTTTTTACTGAT
AAATATTGATGAGTGGAATCTAAATAACATTTCGTCAAAAGCTATTGAAATGTTGCGTGACCCAGATTGGGTAAGTAAAA
TCACCCACCTTGATCAAGATGTACTGAATGTATTATTGAATGGTAAAGTGAAGTTTATTTCGGAGAAATATAATACCCGA
TATAGTATTAACTATGAATTAAAAGACAAAGTTGATAATCCAGTCAATGATGACACCGTGTTTATACACTATGTGGGACC
TACAAAACCTTGGCATGAGTGGGCTGACTATCCGGTGTCACGTAGTTTTTTGATCGCCAAAGCAGCTTCTCCGTGGAGTA
AAGAAGATTTACTTAAACCTGTAAATAGCAATCAGTATCGGTATTGTGCAAAACATAAATTTAAACAAAAGCATTATATG
GCAGGCATTTTTAATTATTTAAAGTATTATAAAGAAAAATGCTTCTAA

Upstream 100 bases:

>100_bases
GCAAGCAGAAGCAAAAGCCACAAAAATCAGGGAAAGAACGATTCGAAAATCGTTGTAACCCGTAACTTATTTTTGCCAAA
ATTTTGGATACAGAATAAAT

Downstream 100 bases:

>100_bases
AGATTTAAGGTTAAAATAATTCATTATGATATATAATAAAACGATAAATGGGGTGAAAGTTTTCATAAAAGATAATGATC
CCTTTTATGAGCAAGTTCTA

Product: lipopolysaccharide 1,3-galactosyltransferase

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVAIASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQ
YNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDV
EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR
YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYM
AGIFNYLKYYKEKCF

Sequences:

>Translated_335_residues
MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVAIASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQ
YNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDV
EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR
YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYM
AGIFNYLKYYKEKCF
>Mature_334_residues
SQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVAIASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQY
NSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVE
WWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRY
SINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMA
GIFNYLKYYKEKCF

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=308, Percent_Identity=57.1428571428571, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1790056, Length=329, Percent_Identity=39.209726443769, Blast_Score=217, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 39416; Mature: 39285

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVAIASILLNNREISCEFH
CCCCCCCCEEEEEEECEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
VFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYH
EEEHHHCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYF
CCCEEEEEECCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHCCCC
NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR
CCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCE
YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKP
EEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHCC
VNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVAIASILLNNREISCEFH
CCCCCCCEEEEEEECEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEE
VFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTKNWTYATYFRFIIADYFYH
EEEHHHCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
KHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYF
CCCEEEEEECCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHCCCC
NAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTR
CCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCE
YSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKP
EEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHCC
VNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKCF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]