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The map label for this gene is sgaE [H]

Identifier: 209396852

GI number: 209396852

Start: 5352627

End: 5353313

Strand: Direct

Name: sgaE [H]

Synonym: ECH74115_5714

Alternate gene names: 209396852

Gene position: 5352627-5353313 (Clockwise)

Preceding gene: 209396286

Following gene: 209398367

Centisome position: 96.06

GC content: 55.9

Gene sequence:

>687_bases
ATGCAAAAGCTAAAACAGCAGGTGTTTGAAGCCAACATGGATCTGCCGCGCTATGGGCTGGTGACCTTTACCTGGGGCAA
CGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTCGCCTACGAAACCATGAAAGCAGACG
ATATGGTGGTGGTCGATATGAGCGGTAAGGTGGTGGAAGGGGAATATCGTCCGTCTTCCGACACCGCGACGCATCTCGAA
CTCTACCGTCGTTATCCGTCGCTTGGCGGCATTGTCCATACCCACTCCACTCATGCTACCGCATGGGCGCAGGCGGGGCT
GGCGATCCCGGCGTTAGGCACCACGCACGCTGACTACTTCTTTGGTGATATCCCGTGTACGCGTGGATTAAGCGAAGAAG
AGGTGCAGGGTGAGTATGAACTGAACACTGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCG
GGAATTGTGGTGTATCAGCACGGACCGTTCGCCTGGGGGAAAGATGCCCACGACGCGGTGCATAACGCGGTAGTGATGGA
AGAAGTGGCGAAAATGGCGTGGATTGCGCGCAGTATTAATCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAAC
ACTTCATGCGTAAGCACGGCCCCAACGCTTATTACGGGCAGAAGTAG

Upstream 100 bases:

>100_bases
TGTGGAGCGAAACGGCGGAAGACCCGGCGGCAGAAGTGGCGAAAGCGCGTGATTGGGTGAAAGCGCGCATGGCGAAAGCG
GGCATGGTGGAGGCGGCATA

Downstream 100 bases:

>100_bases
AACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAGCCCCCTGCG
AAGGGGGCAAAGCAAACTAT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK

Sequences:

>Translated_228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK
>Mature_228_residues
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLE
LYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=98.6842105263158, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=60.8695652173913, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=61.7391304347826, Blast_Score=296, Evalue=8e-82,
Organism=Escherichia coli, GI1789164, Length=138, Percent_Identity=34.0579710144928, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25338; Mature: 25338

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDM
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCEEEEECCCCEEEEEC
SGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDM
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEECCCCCEEEECCCCCEEEEECCCCEEEEEC
SGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTPGIVVYQHGPFAWGKDAHDAV
ECCCCCCCCCCHHHCCCEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCHHHHH
HNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA