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The map label for this gene is thiG [H]

Identifier: 209396837

GI number: 209396837

Start: 5103803

End: 5104618

Strand: Reverse

Name: thiG [H]

Synonym: ECH74115_5458

Alternate gene names: 209396837

Gene position: 5104618-5103803 (Counterclockwise)

Preceding gene: 209397473

Following gene: 209396336

Centisome position: 91.61

GC content: 58.09

Gene sequence:

>816_bases
ATGGCGACCAGATCCTACTTTTTCAGGTTATTGCAGGGGGTTGAGATGTTACGTATTGCGGACAAAACGTTTGATTCACA
TCTGTTTACCGGCACCGGGAAATTCGCCTCTTCACAACTGATGGTAGAGGCAATCCGCGCTTCCGGCAGCCAGCTGGTGA
CGCTGGCGATGAAACGTGTCGACTTGCGCCAGCACAACGACGCTATCCTCGAACCGCTTATCGCGGCGGGTGTGACCCTG
CTGCCAAATACATCCGGGGCGAAAACAGCGGAAGAAGCCATTTTCGCCGCCCATCTGGCTCGTGAAGCGTTAGGCACAAA
CTGGTTAAAATTGGAGATTCACCCTGACGCCCGCTGGCTGTTGCCCGATCCCATCGAAACCCTGAAAGCCGCCGAAACGC
TGGTACAACAGGGATTTGTCGTGCTGCCTTACTGCGGGGCCGATCCGGTATTGTGTAAACGTCTGGAAGAAGTCGGCTGT
GCAGCGGTGATGCCGCTCGGCGCGCCGATTGGCTCGAATCAGGGGCTGGAAACCCGCGCCATGCTGGAGATTATTATCCA
GCAGGCCACAGTTCCGGTGGTTGTCGATGCTGGCATCGGCGTTCCCAGCCATGCCGCGCAGGCGCTGGAAATGGGGGCCG
ACGCGGTGTTAGTGAATACGGCGATTGCCGTCGCGGACGATCCCGTCAACATGGCGAAGGCATTTCGTCTGGCAGTAGAA
GCAGGCCTGCTGGCACCTCAGTCCGGACCGGGCAGCCGCAGTTATTTTGCTCATGCCACCAGCCCGCTGACCGGATTTCT
GGAGGCATCGGTATGA

Upstream 100 bases:

>100_bases
GAACTACTGGAGCAACTCGTTCAACGACAAGCGGGCGCGGCTCTGGCGATTAATCAGCAAATCGTCCCGCGTGAGCAGTG
GACGCAACATATCGTGCAGG

Downstream 100 bases:

>100_bases
AAACCTTCAGCGATCGCTGGCGACAACTGGACTGGGACGACATCCGCCTGCGTATCAACGGCAAAACGGCTGCTGACGTA
GAGCGGGCGCTAAATGCCTC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MATRSYFFRLLQGVEMLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTL
LPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGC
AAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
AGLLAPQSGPGSRSYFAHATSPLTGFLEASV

Sequences:

>Translated_271_residues
MATRSYFFRLLQGVEMLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTL
LPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGC
AAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
AGLLAPQSGPGSRSYFAHATSPLTGFLEASV
>Mature_270_residues
ATRSYFFRLLQGVEMLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTLL
PNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCA
AVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVEA
GLLAPQSGPGSRSYFAHATSPLTGFLEASV

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=255, Percent_Identity=99.6078431372549, Blast_Score=513, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28666; Mature: 28534

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRSYFFRLLQGVEMLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
DLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWL
HHHHCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHH
MLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHH
AGLLAPQSGPGSRSYFAHATSPLTGFLEASV
HCCCCCCCCCCCCCEEEECCCCHHHHHCCCC
>Mature Secondary Structure 
ATRSYFFRLLQGVEMLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRV
CCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
DLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWL
HHHHCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHH
MLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVE
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHHHHHH
AGLLAPQSGPGSRSYFAHATSPLTGFLEASV
HCCCCCCCCCCCCCEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA