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The map label for this gene is pbpG [H]

Identifier: 209396832

GI number: 209396832

Start: 2994214

End: 2995155

Strand: Reverse

Name: pbpG [H]

Synonym: ECH74115_3260

Alternate gene names: 209396832

Gene position: 2995155-2994214 (Counterclockwise)

Preceding gene: 209396981

Following gene: 209399209

Centisome position: 53.75

GC content: 50.96

Gene sequence:

>942_bases
ATGCTCATCATGCCGAAATTTCGAGTTTCTTTATTTAGCCTGGCCCTGATGCTGGCTGTGCCTTTGGCACCGCAGGCCGT
TGCGAAAACGGCAGCCGCTACCACCGCTTCACAACCGGAAATTGCCTCCGGTAGCGCGATGATTGTTGATCTGAATACCA
ACAAAGTGATCTATTCGAACCACCCGGATCTGGTGCGTCCGATTGCGTCTATCAGCAAATTAATGACTGCGATGGTCGTG
CTGGATGCACGACTGCCGCTGGATGAAAAACTAAAAGTGGATATCAGCCAGACGCCGGAGATGAAAGGGGTCTATTCGCG
CGTACGACTGAATAGCGAAATCAGCCGTAAAGATATGCTGTTGCTGGCGCTGATGTCTTCAGAAAACCGCGCGGCGGCAA
GCCTTGCGCACCATTATCCCGGTGGTTACAAAGCCTTTATTAAGGCAATGAATGCGAAAGCGAAATCGCTCGGAATGAAC
AACACGCGCTTTGTTGAACCTACCGGATTGTCGGTGCATAACGTTTCAACCGCCCGTGACTTAACCAAACTGCTCATTGC
CAGCAAACAATATCCGTTGATCGGGCAGTTAAGTACTACCCGGGAAGATATGGCAACCTTCTCTAACCCGACGTATACGC
TGCCGTTCCGCAATACTAATCATCTGGTGTATCGCGATAACTGGAATATCCAGCTAACCAAAACCGGCTTTACCAATGCG
GCGGGCCATTGTCTGGTGATGCGTACGGTTATCAATAATAAACCGGTGGCGCTGGTAGTGATGGATGCGTTTGGCAAATA
CACCCATTTTGCCGATGCCAGCCGCCTGCGTACCTGGATTGAAACCGGTAAAGTGATGCCTGTTCCGGCAGCAGCGTTAA
GCTATAAAAAACAAAAAGCCGCCCAAATGGCGGCGGCGGGGCAGACGGCACAGAACGATTAA

Upstream 100 bases:

>100_bases
CTTTGACGATCGCACTTTTTGCTCGTTATGATGGCGTCGCTAGCCTCAGTAAATCCTTACTCCGGCGGTGCGCAACCCGT
GCGCGTGAACCACTATCTGA

Downstream 100 bases:

>100_bases
GTGAATTCGGATGGCGATACTCTGCCATCCGTAATTTTTACTCCACTTCCTGCCAGTTTTTCCGCTTACTGGTTTTACCG
ATCCCCGGATTCATACTGTT

Product: D-alanyl-D-alanine endopeptidase

Products: NA

Alternate protein names: DD-endopeptidase; Penicillin-binding protein 7; PBP-7 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Sequences:

>Translated_313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND
>Mature_313_residues
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVV
LDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMN
NTRFVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Specific function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082054, Length=310, Percent_Identity=99.6774193548387, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=270, Percent_Identity=28.1481481481481, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082022, Length=287, Percent_Identity=25.4355400696864, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786851, Length=264, Percent_Identity=26.5151515151515, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR018044
- InterPro:   IPR001967 [H]

Pfam domain/function: PF00768 Peptidase_S11 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 34211; Mature: 34211

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECC
HPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDML
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEE
LLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARD
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHH
LTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
HHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCC
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKA
CCCEEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQMAAAGQTAQND
HHHHHCCCCCCCC
>Mature Secondary Structure
MLIMPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSN
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECC
HPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDML
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEE
LLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARD
EEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHH
LTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNA
HHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCC
AGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKA
CCCEEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
AQMAAAGQTAQND
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 6386470; 7721700; 7925376 [H]