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The map label for this gene is yieH [H]

Identifier: 209396591

GI number: 209396591

Start: 4788579

End: 4789244

Strand: Direct

Name: yieH [H]

Synonym: ECH74115_5146

Alternate gene names: 209396591

Gene position: 4788579-4789244 (Clockwise)

Preceding gene: 209400915

Following gene: 209398058

Centisome position: 85.94

GC content: 51.05

Gene sequence:

>666_bases
ATGTCCCAGATAGAAGCGGTATTTTTCGACTGCGACGGTACGCTGGTCGATAGCGAGGTCATTTGCTCTCGCGCCTATGT
CACGATGTTCAGGGAATTTGGCATTCACGTTGACCTGAAAGAGATTTTTACCCGTTTTAAAGGCGTAAAACTCTACGAAA
TCATCGATATCATCAGTAAAGAACAGGGCGTGACGATGGTCCAGGCCGATGCCGAGCACGTTTACCGCGCAGAAGTAGCA
CGTCTGTTTGATACCGAACTTGAGGACATCGCAGGAGCGAACGAATTGCTGGCGAGCATTAACGTGCCGATGTGCGTCGT
CTCTAACGGTCCGGTCAGCAAAATGCAGCATTCGCTTGGTAAGCTCAACATGCTGCACTACTTCCCGGAAAAACTGTTCA
GCGGATATGACATTCAGCGTTGGAAGCCCGATCCGGCATTAATGTTCCATGCGGCAAAAGCGATGAATGTGAACGTTGAA
AACTGCATTCTGGTCGATGACTCCAGCGCTGGCGCTCAATCAGGGATAGATGCCGGAATGGAAGTGTTTTACTTCTGCGC
TGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCGAGCTGTCGCAGCTGCCTGCGCTCTGGAAGG
CACGCGGCTGGAATATAACGCGATAA

Upstream 100 bases:

>100_bases
GGCTGAAGATAACTTTTTTCATTGTATTTGCTTATACGGCAACGATTGCGTCATGCGAATTGGCATGACGAAAACGTTAA
ACTAACGATAAAGGAAAGAC

Downstream 100 bases:

>100_bases
TCGCCTCCTTACTTCAACACTTACACATCAGTCACGCTGGTTTGAGATACAGAACACCAGGTGTCTGATGAGTAAGCGTC
TATTCCCTATGAATAATTTA

Product: 6-phosphogluconate phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFTRFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVA
RLFDTELEDIAGANELLASINVPMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWNITR

Sequences:

>Translated_221_residues
MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFTRFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVA
RLFDTELEDIAGANELLASINVPMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWNITR
>Mature_220_residues
SQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFTRFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVAR
LFDTELEDIAGANELLASINVPMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVEN
CILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWNITR

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=220, Percent_Identity=85, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI87082080, Length=201, Percent_Identity=28.8557213930348, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24827; Mature: 24696

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFTRFKGVKLYEIIDIISK
CCCCEEEEEECCCCEECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCHHHHHHHHHHC
EQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINVPMCVVSNGPVSKMQHSLG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHH
KLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCILVDDSSAGAQSGIDAGM
HHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHHHEECCCCEEEEEECCCCCCCCCCCCCC
EVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWNITR
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLKEIFTRFKGVKLYEIIDIISK
CCCEEEEEECCCCEECHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCHHHHHHHHHHC
EQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINVPMCVVSNGPVSKMQHSLG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHH
KLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCILVDDSSAGAQSGIDAGM
HHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHHHEECCCCEEEEEECCCCCCCCCCCCCC
EVFYFCADPHNKPIVHPKVTTFTELSQLPALWKARGWNITR
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]