Click here to switch to the map view.

The map label for this gene is wzc

Identifier: 209396474

GI number: 209396474

Start: 2773700

End: 2775862

Strand: Reverse

Name: wzc

Synonym: ECH74115_2995

Alternate gene names: 209396474

Gene position: 2775862-2773700 (Counterclockwise)

Preceding gene: 209400491

Following gene: 209395746

Centisome position: 49.82

GC content: 52.84

Gene sequence:

>2163_bases
ATGACAGAAAAAGTAAAACAACATGCCGCTCCGGTAACGGGCAGTGATGAAATCGATATTGGTCGCCTGGTCGGCACCGT
CATTGAAGCGCGCTGGTGGGTGATTGGCATCACCGCTGTATTCGCCCTTTGTGCCGTGGTTTACACCTTCTTCGCCACGC
CGATTTATAGTGCCGACGCACTGGTACAAATCGAGCAAAGCAGCGGCAATTCGTTAGTGCAGGATATCGGATCGGCGTTA
GCCAACAAACCGCCTGCATCGGACGCCGAGATCCAGTTGATTCGTTCGCGCCTGGTGCTTGGTAAAACGGTGGATGATCT
CGACCTCGATATTGCGGTGAGCAAAAACACGTTCCCGATTTTCGGTGCGGGCTGGGATCGCCTGATGGGACGCCAGAACG
AGACGGTGAAAGTGACTACCTTTAACCGTCCGAAAGAGATGGAGGATCAGGTGTTTACGCTTAATGTGCTGGACAACAAA
AACTACACCCTGAGCAGCGATGGCGGCTTTAGCGCCCGTGGGCAAGCGGGCCAGATACTGAAAAAAGAAGGCGTCACGCT
GATGGTTGAAGCCATTCACGCCCGCCCGGGCAGTGAGTTTACCGTCACCAAATACTCCACGCTGGGGATGATCAATCAAC
TGCAAAACAGCCTGACGGTAACGGAGAACGGCAAAGACGCAGGCGTACTGAGCCTGACTTATACCGGTGAAGATCGCGAA
CAGATCCGCGACATTCTTAACAGCATCGCCCGTAACTATCAGGAACAAAATATTGAGCGCAAATCGGCGGAAGCGTCGAA
AAGCCTCGCTTTCCTCGCGCAACAGTTACCGGAAGTACGTAGCCGCCTTGATGTTGCCGAAAACAAACTGAATGCCTTCC
GTCAGGATAAAGATTCTGTTGATCTGCCGCTGGAAGCGAAAGCGGTGCTCGATTCGATGGTGAACATCGACGCCCAGTTG
AACGAACTGACCTTTAAAGAGGCGGAAATCTCCAAGCTGTACACCAAAGTTCACCCCGCGTACCGCACGCTGCTGGAGAA
ACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAATGGTCGCGTAACGGCGATGCCGAAAACCCAGCAGGAAATTGTCC
GTCTGACCCGCGATGTCGAGTCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAG
GCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGTGTGCTAAAACCGAAGAAAGGGCTGAT
TATCCTTGGGGCGATTATCCTTGGCCTGATGCTCTCTATCGTGGGGGTGCTGCTGCGCTCGTTGTTTAATCGCGGCATCG
AAAGCCCGCAGGTGCTGGAAGAACACGGTATCAGCGTCTATGCCAGCATCCCGCTGTCGGAGTGGCAGAAAGCGCGCGAT
AGCGTCAAAACCATCAAAGGGATTAAACGCTATAAACAGAGCCAGCTACTGGCGGTGGGGAATCCAACCGATCTGGCGAT
TGAAGCCATCCGCAGCCTTCGTACCAGTTTGCACTTCGCGATGATGCAGGCGCAGAACAATGTGTTGATGATGACCGGGG
TTAGCCCGTCAATCGGTAAAACCTTTGTCTGCGCCAACCTGGCGGCGGTGATCAGCCAGACCAATAAACGCGTGTTGTTG
ATCGACTGCGATATGCGCAAAGGCTACACCCATGAGCTGTTGGGCACTAATAACGTTAATGGCCTGTCGGAAATTCTGAT
TGGTCAGGGCGATATTACTACAGCTGCTAAACCGACCTCTATTGCCAAATTTGACCTGATCCCGCGCGGTCAGGTACCGC
CAAATCCTTCTGAACTGTTGATGAGCGAACGCTTTGCCGAACTGGTGAACTGGGCGAGTAAAAACTACGACCTGGTGTTG
ATTGATACGCCGCCGATTCTGGCAGTGACCGATGCGGCAATTGTTGGTCGTCATGTCGGAACCACGTTAATGGTGGCGCG
TTATGCGGTCAACACATTGAAAGAAGTGGAAACCAGTCTGAGCCGCTTTGAGCAAAACGGTATTCCGGTGAAAGGGGTGA
TTCTGAACTCCATCTTCCGCCGCGCCAGCGCGTATCAGGATTATGGCTATTACGAATACGAATATAAGTCGGATGCGAAA
TAA

Upstream 100 bases:

>100_bases
CGTATCGCAAAAGCCGGGAAACGTTTGCAGCGGTGTACACATTACTTGAACGGTCTGCCCGCCAGTGGGCGCAGGCATTG
AACGCAGAGCAGGTATAAGA

Downstream 100 bases:

>100_bases
CGAGGCCTGCATTCGCACCGCCCCGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCAAGCAAACCAGCTCATAAGCCG
GGAGTACAACCTATGAAAGA

Product: tyrosine kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALCAVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSAL
ANKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDRE
QIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQL
NELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITE
ASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARD
SVKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLL
IDCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVL
IDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK

Sequences:

>Translated_720_residues
MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALCAVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSAL
ANKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNK
NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDRE
QIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQL
NELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITE
ASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARD
SVKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLL
IDCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVL
IDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK
>Mature_719_residues
TEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALCAVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALA
NKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNKN
YTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDREQ
IRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLN
ELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITEA
STVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARDS
VKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLI
DCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVLI
DTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK

Specific function: Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium. Phosphorylates udg

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family

Homologues:

Organism=Escherichia coli, GI87082032, Length=720, Percent_Identity=99.3055555555556, Blast_Score=1466, Evalue=0.0,
Organism=Escherichia coli, GI1787216, Length=709, Percent_Identity=51.6220028208745, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WZC_ECO57 (Q8X7L9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A90987
- PIR:   D85832
- RefSeq:   NP_288566.2
- RefSeq:   NP_310892.2
- ProteinModelPortal:   Q8X7L9
- SMR:   Q8X7L9
- EnsemblBacteria:   EBESCT00000027907
- EnsemblBacteria:   EBESCT00000056839
- GeneID:   912525
- GeneID:   956738
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3224
- KEGG:   ecs:ECs2865
- GeneTree:   EBGT00050000010177
- HOGENOM:   HBG309770
- OMA:   IRMTEKV
- ProtClustDB:   PRK11519
- BioCyc:   ECOL83334:ECS2865-MONOMER
- BRENDA:   2.7.10.1
- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR003856
- TIGRFAMs:   TIGR01007

Pfam domain/function: PF01656 CbiA; PF02706 Wzz

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 79396; Mature: 79265

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x220fea14)-; HASH(0x20618c8c)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALCAVVYTFFATPIYSADA
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPI
EEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
FGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNKNYTLSSDGGFSARGQAGQIL
ECCCHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCHHHHH
KKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDRE
HHCCCEEEEEEHHCCCCCCEEEEEHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCHH
QIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNG
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
RVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITEASTVGDVRIVDPAITQPGVL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCCCCCC
KPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARD
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCHHHHHHHHH
SVKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGK
HHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
TFVCANLAAVISQTNKRVLLIDCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTS
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCC
IAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVG
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECHHHHHHHHHH
TTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
>Mature Secondary Structure 
TEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALCAVVYTFFATPIYSADA
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPI
EEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEE
FGAGWDRLMGRQNETVKVTTFNRPKEMEDQVFTLNVLDNKNYTLSSDGGFSARGQAGQIL
ECCCHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCHHHHH
KKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDRE
HHCCCEEEEEEHHCCCCCCEEEEEHHHHHHHHHHHHCEEEECCCCCCEEEEEEECCCCHH
QIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNG
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
RVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITEASTVGDVRIVDPAITQPGVL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCCCEEEECCCCCCCCCC
KPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARD
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCHHHHHHHHH
SVKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGK
HHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
TFVCANLAAVISQTNKRVLLIDCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTS
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCC
IAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVG
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECHHHHHHHHHH
TTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796