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The map label for this gene is putP [H]

Identifier: 209396363

GI number: 209396363

Start: 1262403

End: 1263911

Strand: Direct

Name: putP [H]

Synonym: ECH74115_1253

Alternate gene names: 209396363

Gene position: 1262403-1263911 (Clockwise)

Preceding gene: 209399177

Following gene: 209398376

Centisome position: 22.66

GC content: 54.08

Gene sequence:

>1509_bases
ATGGCTATTAGCACACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCG
ATCAACGAAAAACTTTGACGACTATATTCTGGGCGGACGTAGCCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCAT
CGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCGCGGTTTTTCTTTCCGGGATTTCCGAAAGTTGGATCGCCATT
GGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAATAACGC
CTTAACATTACCGGATTATTTCACCGGGCGTTTTGAAGATAAAAGCCGCATTTTGCGCATTATCTCCGCGCTGGTGATTT
TGCTGTTCTTCACCATTTATTGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTACCTTTGGCATGAGCTAC
GAAACGGCTCTGTGGGCGGGAGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTCCTCGCGGTGAGCTGGACTGA
CACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTGACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTG
ACTCGCTGGAAGTGATCAAACAAAAGAGCATCGAAAACGTGGATATGCTCAAAGGGCTGAACTTTGTCGCCATTATCTCG
CTGATGGGGTGGGGGCTGGGTTACTTCGGGCAACCGCACATCCTGGCGCGTTTTATGGCGGCGGATTCTCACCACAGCAT
CGTCCATGCGCGTCGTATCAGTATGACCTGGATGATCCTCTGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGTATCG
CTTACTTTAACGAGCACCCGTCGGTAGCTGGTGCGGTAAACCAGAACGCCGAGCGCGTGTTTATCGAACTGGCGCAAATT
CTGTTTAACCCGTGGATTGCCGGGATTCTGCTGTCGGCGATTCTGGCGGCGGTAATGTCAACGTTAAGTTGCCAGCTGCT
GGTATGTTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAACTGGTGTGGG
TAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGTTGGCAGCGAACCCGGAAAACCGCGTGCTGGGCTTA
GTGAGCTACGCGTGGGCAGGCTTTGGCGCGGCGTTTGGTCCGGTGGTGCTGTTTTCGGTGATGTGGTCACGCATGACGCG
CAACGGTGCGCTGGCGGGGATGATCATCGGTGCGCTGACGGTGATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACG
AAATTATTCCGGGCTTTATCTTTGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCACCGTCAGCGGCGATG
CAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAGGCTAA

Upstream 100 bases:

>100_bases
ACGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAA
TAATTTTTTGGAGACTTTAG

Downstream 100 bases:

>100_bases
GCTGAAGAGTAATTAGTTATCTTTTGCTTTTTAACCCGAATGTTCCCTGCCATGCGGTATAAGTTGCCTGGCAGGGAGTG
TTCGGGGGCAATTGCTATTT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
QKRFAEADAHYHSAPPSRLQEG

Sequences:

>Translated_502_residues
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAI
GLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQI
LFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGL
VSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
QKRFAEADAHYHSAPPSRLQEG
>Mature_501_residues
AISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIG
LTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYE
TALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIISL
MGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNEHPSVAGAVNQNAERVFIELAQIL
FNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLV
SYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQ
KRFAEADAHYHSAPPSRLQEG

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=490, Percent_Identity=45.7142857142857, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI110835708, Length=491, Percent_Identity=24.847250509165, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI4507035, Length=374, Percent_Identity=22.7272727272727, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1787251, Length=501, Percent_Identity=99.4011976047904, Blast_Score=998, Evalue=0.0,
Organism=Escherichia coli, GI87082237, Length=460, Percent_Identity=27.3913043478261, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1790503, Length=451, Percent_Identity=26.3858093126386, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1790113, Length=508, Percent_Identity=23.4251968503937, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533094, Length=425, Percent_Identity=25.6470588235294, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459584, Length=389, Percent_Identity=22.879177377892, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645928, Length=357, Percent_Identity=25.7703081232493, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221459588, Length=427, Percent_Identity=24.1217798594848, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24651741, Length=370, Percent_Identity=23.5135135135135, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24650192, Length=402, Percent_Identity=21.6417910447761, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221459582, Length=401, Percent_Identity=24.6882793017456, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221459586, Length=378, Percent_Identity=22.4867724867725, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640370, Length=356, Percent_Identity=24.1573033707865, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24651739, Length=386, Percent_Identity=22.020725388601, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 54331; Mature: 54200

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFED
EECCCHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCEEECCCHHCCCCCC
KSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
HEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNEHP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QKRFAEADAHYHSAPPSRLQEG
HHHHHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCEE
LMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFED
EECCCHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCEEECCCHHCCCCCC
KSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
HEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHH
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNEHP
HHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SVAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHHHH
MWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QKRFAEADAHYHSAPPSRLQEG
HHHHHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]